SPiNE Target Progress Summary

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Purification Batch Record for FR855B-32-327-15.1-SEMA-GF


GENERAL
Purification ID: FR855B-32-327-15.1-SEMA-GF Created: 11-25-2013 Modified: 05-05-2016
Expression ID: FR855B-32-327-15.1-SeMa Target ID: FR855B Source Tube ID:
Researchers: *Rong Xiao

PURIFICATION
Date Purified: 11-21-2013 Stored Purification Yield: (mg)
Calculated Purification Yield (Final Conc. * Final Vol.): 92 (mg)
Solubility of Purified Protein: Concentratable: (0-5)
Method: Superdex75 Process yield: 0 mg/L
Purifcation buffer: Low Salt Final buffer: Low Salt
Final concentration: 4.6 mg/mL Final volume: 20 mL

QUALITATIVE
Oligomerization State: Dimer Determined by: Gel filtration
Link to SDS-PAGE Image 1: data/F/FR855B/purification/FR855B-32-327-15.1-SeMa-GF.sds.jpg
Link to SDS-PAGE Image 2: data/F/FR855B/purification/FR855B-32-327-15.1-SeMa-GF.sds2.jpg
Link to Mass Spectrum Image: data/F/FR855B/purification/FR855B-32-327-15.1-SeMa-GF.mass.jpg
Link to Chromatogram Image: data/F/FR855B/purification/FR855B-32-327-15.1-SeMa-GF.chro.jpg
Purification Comments

mass spec is off, about 3kDa smaller than expected




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Last modified 06-15-06