SPiNE Target Progress Summary

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Purification Batch Record for HR2943-1-139-15.4-SEMB-GF


GENERAL
Purification ID: HR2943-1-139-15.4-SEMB-GF Created: 12-03-2010 Modified: 02-18-2014
Expression ID: HR2943-1-139-15.4-SeMb Target ID: HR2943 Source Tube ID:
Researchers: *Daya Patel, Rong Xiao

PURIFICATION
Date Purified: 11-29-2010 Stored Purification Yield: 0 (mg)
Calculated Purification Yield (Final Conc. * Final Vol.): 21 (mg)
Solubility of Purified Protein: Concentratable: (0-5)
Method: Superdex75 Process yield: 0 mg/L
Purifcation buffer: Low Salt Final buffer: Low Salt
Final concentration: 0.7 mg/mL Final volume: 30.0 mL

QUALITATIVE
Oligomerization State: Dimer Determined by: Gel filtration
Link to SDS-PAGE Image 1: data/H/HR2943/purification/HR2943-1-139-15.4-SeMb-GF.sds.jpg
Link to SDS-PAGE Image 2: data/H/HR2943/purification/HR2943-1-139-15.4-SeMb-GF.sds2.jpg
Link to Mass Spectrum Image: data/H/HR2943/purification/HR2943-1-139-15.4-SeMb-GF.mass.jpg
Link to Chromatogram Image: data/H/HR2943/purification/HR2943-1-139-15.4-SeMb-GF.chro.jpg
Purification Comments

Protein started precipitating during concentration.




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Last modified 06-15-06