SPiNE Target Progress Summary

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Purification Batch Record for HR3376H-60-704-C261S,R307G,C501A,C527A,C573R-MBP3.1-NCDAILVA-TV


GENERAL
Purification ID: HR3376H-60-704-C261S,R307G,C501A,C527A,C573R-MBP3.1-NCDAILVA-TV Created: 12-08-2014 Modified: 12-08-2014
Expression ID: HR3376H-60-704-C261S,R307G,C501A,C527A,C573R-MBP3.1-NCDAILVa Target ID: HR3376H Source Tube ID:
Researchers: *Lei Mao, Rong Xiao

PURIFICATION
Date Purified: 12-05-2014 Stored Purification Yield: (mg)
Calculated Purification Yield (Final Conc. * Final Vol.): 6.9 (mg)
Solubility of Purified Protein: Concentratable: (0-5)
Method: 5 mL HisTrap Process yield: 0 mg/L
Purifcation buffer: Binding Buffer Final buffer: Binding Buffer
Final concentration: 0.69 mg/mL Final volume: 10.0 mL

QUALITATIVE
Oligomerization State: . Determined by: Ni-NTA
Link to SDS-PAGE Image 1: data/H/HR3376H/purification/HR3376H-60-704-C261S,R307G,C501A,C527A,C573R-MBP3.1-NCDAILVa-TV.sds.jpg
Link to SDS-PAGE Image 2: data/H/HR3376H/purification/HR3376H-60-704-C261S,R307G,C501A,C527A,C573R-MBP3.1-NCDAILVa-TV.sds2.jpg
Link to Chromatogram Image: data/H/HR3376H/purification/HR3376H-60-704-C261S,R307G,C501A,C527A,C573R-MBP3.1-NCDAILVa-TV.chro.jpg
Purification Comments




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Last modified 06-15-06