SPiNE Target Progress Summary

JavaScript Menu Courtesy of Milonic.com

Purification Batch Record for HR3376I-15TOPO.1-[NC-R1-DNC,NC-R2-DNC]-NI


GENERAL
Purification ID: HR3376I-15TOPO.1-[NC-R1-DNC,NC-R2-DNC]-NI Created: 09-17-2013 Modified: 02-18-2014
Expression ID: HR3376I-15TOPO.1-NC-R1-DNC Target ID: HR3376I Source Tube ID:
Researchers: *Lei Mao, Rong Xiao

PURIFICATION
Date Purified: 09-16-2013 Stored Purification Yield: (mg)
Calculated Purification Yield (Final Conc. * Final Vol.): 4 (mg)
Solubility of Purified Protein: Concentratable: (0-5)
Method: 5mL HisTrap Process yield: 0 mg/L
Purifcation buffer: TRAP1 Ni Wash Final buffer: TRAP1 Ni Elu
Final concentration: 1 mg/mL Final volume: 4.0 mL

QUALITATIVE
Oligomerization State: 0 Determined by: Ni-NTA
Link to SDS-PAGE Image 1: data/H/HR3376I/purification/HR3376I-15TOPO.1-[NC-R1-DNC,NC-R2-DNC]-Ni.sds.jpg
Purification Comments

the buffer composition is based on PH project protocol




Associated records

  Create a new structure record

There are no PSTs (protein sample tubes) associated with this purification batch.
Ideally, all structure records should be associated with a PST.
If a pst record does not exist for the sample you are working with,
you may create a structure record for a mock pst record HR3376I.000

Create a new (NMR record) or (X-ray record)

Create a CD record for this purification batch
Create a new aggregation screen for this purification batch
Create a new sample tube
Create a new batch from this one
Upload RDC data
Last modified 06-15-06