SPiNE Target Progress Summary

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Purification Batch Record for HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDA-TV


GENERAL
Purification ID: HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDA-TV Created: 06-11-2014 Modified: 12-02-2014
Expression ID: HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDa Target ID: HR3376I Source Tube ID:
Researchers: *Lei Mao, Rong Xiao

PURIFICATION
Date Purified: 06-11-2014 Stored Purification Yield: (mg)
Calculated Purification Yield (Final Conc. * Final Vol.): 3.2 (mg)
Solubility of Purified Protein: Concentratable: (0-5)
Method: 5mL HisTrap Process yield: 0 mg/L
Purifcation buffer: Binding Buffer Final buffer: Elution Buffer
Final concentration: 0.2 mg/mL Final volume: 16.0 mL

QUALITATIVE
Oligomerization State: . Determined by: Ni-NTA
Link to SDS-PAGE Image 1: data/H/HR3376I/purification/HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDa-TV.sds.jpg
Link to SDS-PAGE Image 2: data/H/HR3376I/purification/HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDa-TV.sds2.jpg
Link to Mass Spectrum Image: data/H/HR3376I/purification/HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDa-TV.mass.jpg
Link to Chromatogram Image: data/H/HR3376I/purification/HR3376I-60-704-S170C-C261S-R307G-C501A-C527A-C573R-MBP3.1-NCDa-TV.chro.jpg
Purification Comments




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Last modified 06-15-06