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FR822A   (MRG15)

2 targets assoicated with this target.
FR822parentselected
FR822Bsibling(253-423)expressed

database refs UniProt: Q9Y0I1 (EAF3_DROME)   |   NCBI gene: 41850   |   refSeq: NP_650442.1
status NMR structure and crystal hits
structures 2LRQ (NMR)  
properties length: 88 aa   |   mass: 10.30 kD   |   pI: 8.57   |   ext: 23950   |   organism: Drosophila melanogaster
classes Epigenetics: Lid Complex, PSI-Biology
projects LID complex     PSI:Biology Partner - EpiG    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF11717 details   54160 details   2.30.30.270 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (FR822A).
Display constructs associated with this target (FR822A) and all other associated targets (FR822, FR822B).


Domain and UniProt Features
    CATH 2.30.30.270
    SuperFamily 54160
    PFAM PF05712
    UniProt DOMAIN:MRG
    MRG. {ECO:0000270|PROSITE- domain_number-1


NESG targets
    FR822A
    FR822B

FR822

       PDB seq homology



Constructs and Most Advanced Status
   
FR822A-1-94-TEV.*

   
FR822A-1-94-Av6HT.*

   
FR822A-1-81-Av6HT.*

   
FR822A-1-121-Av6HT.*

   
FR822A-1-81-TEV.*

   
FR822A-6-76-TEV.*

   
FR822A-6-94-Av6HT.*

   
FR822A-6-76-Av6HT.*

   
FR822A-6-94-TEV.*

   
FR822A-11-94-TEV.*

   
FR822A-11-81-TEV.*

   
FR822A-11-81-Av6HT.*

   
FR822A-11-94-Av6HT.*

   
FR822A-17-94-Av6HT.*

   
FR822A-17-94-TEV.*

   
FR822B-248-424-TEV.*

   
FR822B-248-424-Av6HT.*

   
FR822B-253-424-TEV.*

   
FR822B-253-424-Av6HT.*

   
FR822B-302-424-TEV.*

   
FR822B-302-424-Av6HT.*



most recent record
4 years, 14 days ago --     aggregation screening [dimer,33.67 kD,96.0%]

2011-03-30    target: FR822A
2011-05-04    construct: FR822A-1-81-TEV.1
2011-05-17    expression [ 5, 3, not tested ]: FR822A-1-81-TEV.1-ss
2011-06-07    expression [ 5, 3, not tested ]: FR822A-1-81-TEV.1-SeMb
2011-06-15    purification [24.0 mg]: FR822A-1-81-TEV.1-SeMb-GF
2011-06-28     FR822A.001 @ Rutgers; 0.4 ml at 2.07 mg/mL
2011-06-28     FR822A.002 @ Rutgers; Unknown volume and concentration
2011-06-07    expression [ 5, 3, not tested ]: FR822A-1-81-TEV.1-SeMa
2011-06-15    purification [24.0 mg]: FR822A-1-81-TEV.1-SeMa-GF
2011-06-08    expression [ 4, 4, not tested ]: FR822A-1-81-TEV.1-NC5a
2011-06-15    purification [46.3 mg]: FR822A-1-81-TEV.1-NC5a-Ni
2011-06-21     FR822A.009 @ Rutgers; 1.85 ml at 0.861856 mM
2011-07-01     FR822A.014 @ Rutgers; 0.04 ml at 0.65 mM
2011-07-22    HSQC [promising]: FR822A.014.600MHz.20C
2011-06-08    expression [ 4, 4, not tested ]: FR822A-1-81-TEV.1-NC5b
2011-06-15    purification [46.3 mg]: FR822A-1-81-TEV.1-NC5b-Ni
2011-05-04    construct: FR822A-1-81-TEV.2
2011-05-17    expression [ 5, 3, not tested ]: FR822A-1-81-TEV.2-ss
2011-05-04    construct: FR822A-1-94-TEV.3
2011-05-17    expression [ 4, 4, not tested ]: FR822A-1-94-TEV.3-ss
2011-06-07    expression [ 5, 3, not tested ]: FR822A-1-94-TEV.3-SeMb
2011-06-15    purification [22.0 mg]: FR822A-1-94-TEV.3-SeMb-GF
2011-06-28     FR822A.003 @ Rutgers; 0.4 ml at 1.89 mg/mL
2011-06-28     FR822A.004 @ HWI; 0.45 ml at 7 mg/mL
2011-06-07    expression [ 5, 3, not tested ]: FR822A-1-94-TEV.3-SeMa
2011-06-15    purification [22.0 mg]: FR822A-1-94-TEV.3-SeMa-GF
2011-06-08    expression [ 4, 4, not tested ]: FR822A-1-94-TEV.3-NC5b
2011-06-15    purification [40.2 mg]: FR822A-1-94-TEV.3-NC5b-Ni
2011-06-08    expression [ 4, 4, not tested ]: FR822A-1-94-TEV.3-NC5a
2011-06-15    purification [40.2 mg]: FR822A-1-94-TEV.3-NC5a-Ni
2011-06-21     FR822A.010 @ Rutgers; 1.7 ml at 0.910268 mM
2011-07-01     FR822A.015 @ Rutgers; 0.04 ml at 0.75 mM
2011-07-22    HSQC [promising]: FR822A.015.600MHz.20C
2011-05-04    construct: FR822A-1-94-TEV.4
2011-05-17    expression [ 4, 4, not tested ]: FR822A-1-94-TEV.4-ss
2011-05-04    construct: FR822A-11-94-TEV.7
2011-05-17    expression [ 4, 4, not tested ]: FR822A-11-94-TEV.7-ss
2011-06-07    expression [ 4, 4, not tested ]: FR822A-11-94-TEV.7-SeMa
2011-06-15    purification [8.8 mg]: FR822A-11-94-TEV.7-SeMa-GF
2011-06-28     FR822A.005 @ Rutgers; 0.4 ml at 1.93 mg/mL
2011-06-28     FR822A.006 @ Rutgers; 0.3 ml at 3.2 mg/mL
2011-06-07    expression [ 4, 3, not tested ]: FR822A-11-94-TEV.7-SeMb
2011-06-15    purification [8.8 mg]: FR822A-11-94-TEV.7-SeMb-GF
2011-06-08    expression [ 5, 4, not tested ]: FR822A-11-94-TEV.7-NC5a
2011-06-15    purification [27.7 mg]: FR822A-11-94-TEV.7-NC5a-Ni
2011-06-21     FR822A.011 @ UGA; 1.3 ml at 1.5461 mM
2011-07-01     FR822A.016 @ Rutgers; 0.04 ml at 0.75 mM
2011-07-22    HSQC [good]: FR822A.016.600MHz.20C
2011-06-08    expression [ 5, 4, not tested ]: FR822A-11-94-TEV.7-NC5b
2011-06-15    purification [27.7 mg]: FR822A-11-94-TEV.7-NC5b-Ni
2011-08-03    expression [ 5, 3, not tested ]: FR822A-11-94-TEV.7-NCa
2011-08-11    purification [13.1 mg]: FR822A-11-94-TEV.7-NCa-Ni
    FR822A.020 @ Rutgers; Unknown volume and concentration
2011-08-11    purification [10.2 mg]: FR822A-11-94-TEV.7-NCa-GF
2011-08-24     FR822A.022 @ UGA; 0.3 ml at 0.23 mM
2011-09-15    HSQC [good]: FR822A.022.600MHz.25C
2011-08-15    purification [7.4 mg]: FR822A-11-94-TEV.7-NCa-TV
2011-08-03    expression [ 5, 3, not tested ]: FR822A-11-94-TEV.7-NC5c
2011-08-11    purification [12.5 mg]: FR822A-11-94-TEV.7-NC5c-Ni
2011-08-11    purification [7.2 mg]: FR822A-11-94-TEV.7-NC5c-GF
2011-08-24     fr822a.023 @ UGA; 0.5 ml at 0.14 mM
2011-09-15    HSQC [good]: FR822A.023.600MHz.25C
2011-08-15    purification [5.9 mg]: FR822A-11-94-TEV.7-NC5c-TV
2011-11-08    expression [ 5, 3, not tested ]: FR822A-11-94-TEV.7-NCc
2011-11-14    purification [16.6 mg]: FR822A-11-94-TEV.7-NCc-Ni
2011-11-14    purification [16.0 mg]: FR822A-11-94-TEV.7-NCc-GF
2011-11-14    purification [10.9 mg]: FR822A-11-94-TEV.7-NCc-TV
2011-11-08    expression [ 5, 3, not tested ]: FR822A-11-94-TEV.7-NCb
2011-11-14    purification [16.6 mg]: FR822A-11-94-TEV.7-NCb-Ni
2011-11-14    purification [16.0 mg]: FR822A-11-94-TEV.7-NCb-GF
2011-11-14    purification [10.9 mg]: FR822A-11-94-TEV.7-NCb-TV
2011-11-18     FR822A.024 @ UGA; 0.3 ml at 0.96 mM
2011-11-21    HSQC [good]: FR822A.024.600MHz.20C
2011-11-18     FR822A.025 @ UGA; 0.3 ml at 0.96 mM
2012-04-11     NMR structure: 2lrq (PDB)
2011-05-04    construct: FR822A-11-94-TEV.8
2011-05-17    expression [ 3, 4, not tested ]: FR822A-11-94-TEV.8-ss
2011-05-04    construct: FR822A-17-94-TEV.9
2011-05-17    expression [ 2, 0, not tested ]: FR822A-17-94-TEV.9-ss
2011-05-04    construct: FR822A-17-94-TEV.10
2011-05-17    expression [ 2, 0, not tested ]: FR822A-17-94-TEV.10-ss
2011-05-04    construct: FR822A-6-76-TEV.11
2011-05-17    expression [ 3, 0, not tested ]: FR822A-6-76-TEV.11-ss
2011-05-04    construct: FR822A-6-76-TEV.12
2011-05-17    expression [ 3, 0, not tested ]: FR822A-6-76-TEV.12-ss
2011-05-04    construct: FR822A-6-94-TEV.13
2011-05-17    expression [ 3, 5, not tested ]: FR822A-6-94-TEV.13-ss
2011-05-04    construct: FR822A-6-94-TEV.14
2011-05-17    expression [ 3, 5, not tested ]: FR822A-6-94-TEV.14-ss
2011-06-07    expression [ 4, 4, not tested ]: FR822A-6-94-TEV.14-SeMa
2011-06-15    purification [11.0 mg]: FR822A-6-94-TEV.14-SeMa-GF
2011-06-28     FR822A.007 @ Rutgers; 0.4 ml at 2.48 mg/mL
2011-06-28     FR822A.008 @ Rutgers; 0.45 ml at 2.88 mg/mL
2011-06-07    expression [ 4, 4, not tested ]: FR822A-6-94-TEV.14-SeMb
2011-06-15    purification [11.0 mg]: FR822A-6-94-TEV.14-SeMb-GF
2011-06-08    expression [ 4, 4, not tested ]: FR822A-6-94-TEV.14-NC5a
2011-06-15    purification [29.7 mg]: FR822A-6-94-TEV.14-NC5a-Ni
2011-06-21     FR822A.012 @ Rutgers; 1.27 ml at 0.873266 mM
2011-07-01     FR822A.017 @ Rutgers; 0.04 ml at 0.74 mM
2011-07-22    HSQC [good]: FR822A.017.600MHz.20C
2011-06-08    expression [ 4, 4, not tested ]: FR822A-6-94-TEV.14-NC5b
2011-06-15    purification [29.7 mg]: FR822A-6-94-TEV.14-NC5b-Ni
2011-05-04    construct: FR822A-11-81-TEV.15
2011-05-17    expression [ 3, 5, not tested ]: FR822A-11-81-TEV.15-ss
2011-05-04    construct: FR822A-11-81-TEV.16
2011-05-17    expression [ 4, 4, not tested ]: FR822A-11-81-TEV.16-ss
2011-06-07    expression [ 4, 3, not tested ]: FR822A-11-81-TEV.16-SeMb
2011-07-07    purification [6.8 mg]: FR822A-11-81-TEV.16-SeMb-GF
2011-07-14     FR822A.019 @ Rutgers; 0.45 ml at 3.6 mg/mL
2011-06-07    expression [ 4, 3, not tested ]: FR822A-11-81-TEV.16-SeMa
2011-07-07    purification [6.8 mg]: FR822A-11-81-TEV.16-SeMa-GF
2011-06-08    expression [ 4, 3, not tested ]: FR822A-11-81-TEV.16-NC5b
2011-06-15    purification [10.6 mg]: FR822A-11-81-TEV.16-NC5b-Ni
2011-06-08    expression [ 4, 3, not tested ]: FR822A-11-81-TEV.16-NC5a
2011-06-15    purification [10.6 mg]: FR822A-11-81-TEV.16-NC5a-Ni
2011-06-21     FR822A.013 @ Rutgers; 0.4 ml at 1.107 mM
2011-07-01     FR822A.018 @ Rutgers; 0.04 ml at 0.54 mM
2011-07-22    HSQC [good]: FR822A.018.600MHz.20C
2012-09-07    construct: FR822A-1-94-Av6HT.20
2012-09-28    expression [ 5, 5, not tested ]: FR822A-1-94-Av6HT.20-ss
2012-09-07    construct: FR822A-11-81-Av6HT.21
2012-09-28    expression [ 4, 3, not tested ]: FR822A-11-81-Av6HT.21-ss
2012-10-10    expression [ 5, 1, not tested ]: FR822A-11-81-Av6HT.21-N14b
2012-10-10    expression [ 5, 1, not tested ]: FR822A-11-81-Av6HT.21-N14a
2012-10-10    expression [ 5, 1, not tested ]: FR822A-11-81-Av6HT.21-N14c
2012-09-07    construct: FR822A-11-81-Av6HT.22
2012-09-28    expression [ 4, 2, not tested ]: FR822A-11-81-Av6HT.22-ss
2012-09-24    construct: FR822A-1-121-Av6HT.33
2012-10-25    expression [ 5, 5, not tested ]: FR822A-1-121-Av6HT.33-ss
2012-12-20    expression [ 5, 4, not tested ]: FR822A-1-121-AV6HT.33-SeMa
2012-12-20    expression [ 4, 5, not tested ]: FR822A-1-121-AV6HT.33-NC5a
2013-02-02    purification [7.2 mg]: FR822A-1-121-AV6HT.33-NC5a-Ni
2013-02-02    purification [10.8 mg]: FR822A-1-121-AV6HT.33-NC5a-TV
2013-02-02    purification [4.8 mg]: FR822A-1-121-AV6HT.33-NC5a-GF
2013-02-18     FR822A.030 @ Rutgers; Unknown volume and concentration
2013-02-18     FR822A.029 @ Rutgers; Unknown volume and concentration
2013-04-05    HSQC [promising]: FR822A.029.600MHz.20C
2013-05-08    expression [ 4, 5, not tested ]: FR822A-1-121-Av6HT.33-LB-R1
2013-05-16    purification [27.6 mg]: FR822A-1-121-Av6HT.33-LB-R1-Ni
2013-05-20    purification [5.6 mg]: FR822A-1-121-Av6HT.33-LB-R1-GF
2013-05-22     FR822A.032 @ Rutgers; 2.5 ml at 1 mg/mL
2013-04-24    expression [ 3, 5, not tested ]: FR822A-1-121-Av6HT.33-LB-R2
2012-09-07    construct: FR822A-6-76-Av6HT.28
2012-09-28    expression [ 5, 1, not tested ]: FR822A-6-76-Av6HT.28-ss
2012-09-07    construct: FR822A-6-94-Av6HT.29
2012-09-28    expression [ 5, 3, not tested ]: FR822A-6-94-Av6HT.29-ss
2012-10-10    expression [ 5, 3, not tested ]: FR822A-6-94-Av6HT.29-N14b
2012-10-10    expression [ 5, 4, not tested ]: FR822A-6-94-Av6HT.29-N14a
2012-09-07    construct: FR822A-6-94-Av6HT.30
2012-09-28    expression [ 5, 3, not tested ]: FR822A-6-94-Av6HT.30-ss
2012-09-07    construct: FR822A-11-94-Av6HT.23
2012-09-28    expression [ 5, 5, not tested ]: FR822A-11-94-Av6HT.23-ss
2012-10-10    expression [ 5, 5, not tested ]: FR822A-11-94-Av6HT.23-N14a
2013-01-02    purification [3.6 mg]: FR822A-11-94-Av6HT.23-N14a-Ni
2013-01-04     FR822A.027 @ Rutgers; 2.25 ml at 0.7 mg/mL
2012-09-07    construct: FR822A-6-76-Av6HT.27
2012-09-28    expression [ 5, 1, not tested ]: FR822A-6-76-Av6HT.27-ss
2012-09-07    construct: FR822A-17-94-Av6HT.26
2012-09-28    expression [ 5, 4, not tested ]: FR822A-17-94-Av6HT.26-ss
2012-10-10    expression [ 5, 4, not tested ]: FR822A-17-94-Av6HT.26-N14a
2012-09-07    construct: FR822A-17-94-Av6HT.25
2012-09-28    expression [ 5, 4, not tested ]: FR822A-17-94-Av6HT.25-ss
2012-09-07    construct: FR822A-11-94-Av6HT.24
2012-09-28    expression [ 4, 2, not tested ]: FR822A-11-94-Av6HT.24-ss
2012-09-07    construct: FR822A-1-81-Av6HT.17
2012-09-28    expression [ 4, 3, not tested ]: FR822A-1-81-Av6HT.17-ss
2012-09-07    construct: FR822A-1-81-Av6HT.18
2012-09-28    expression [ 4, 3, not tested ]: FR822A-1-81-Av6HT.18-ss
2012-10-10    expression [ 5, 2, not tested ]: FR822A-1-81-Av6HT.18-N14b
2012-10-10    expression [ 5, 2, not tested ]: FR822A-1-81-Av6HT.18-N14a
2012-10-10    expression [ 5, 3, not tested ]: FR822A-1-81-Av6HT.18-N14c
2012-09-07    construct: FR822A-1-94-Av6HT.19
2012-09-28    expression [ 5, 5, not tested ]: FR822A-1-94-Av6HT.19-ss
2012-10-10    expression [ 5, 5, not tested ]: FR822A-1-94-Av6HT.19-N14a
2013-01-02    purification [9.0 mg]: FR822A-1-94-Av6HT.19-N14a-Ni
2013-01-29     FR822A.028 @ Rutgers; 1.5 ml at 0.26 mg/mL
2013-01-04     FR822A.026 @ Rutgers; 3.25 ml at 1.6 mg/mL
2012-09-20    construct: FR822A-1-121-Av6HT.31
2012-10-25    expression [ 5, 5, not tested ]: FR822A-1-121-Av6HT.31-ss
2013-01-16    expression [ 5, 5, not tested ]: FR822A-1-121-Av6HT.31-SeMa
2013-08-26    purification [16.2 mg]: FR822A-1-121-Av6HT.31-SeMa-GF
2013-08-28    purification [3.6 mg]: FR822A-1-121-Av6HT.31-SeMa-TV
2013-09-10     aggregation screening [dimer,33.67 kD,96.0%]
2013-08-29     FR822A.033 @ HWI; 0.45 ml at 8.11 mg/mL
2013-01-16    expression [ 5, 4, not tested ]: FR822A-1-121-Av6HT.31-NC5a
2013-03-04    purification [29.0 mg]: FR822A-1-121-Av6HT.31-NC5a-Ni
2013-03-04    purification [14.7 mg]: FR822A-1-121-Av6HT.31-NC5a-TV
2013-03-15     FR822A.021 @ Rutgers; 0.04 ml at 1.2 mM
2013-04-12    HSQC [poor]: FR822A.021.600MHz.20C
2013-03-15     FR822A.031 @ Rutgers; 0.46 ml at 1.2 mM


Available samples:

LB samples
FR822A.032 @ Rutgers


SeMet samples
FR822A.006 @ Rutgers FR822A.004 @ HWI FR822A.002 @ Rutgers FR822A.001 @ Rutgers
FR822A.019 @ Rutgers FR822A.033 @ HWI FR822A.005 @ Rutgers FR822A.003 @ Rutgers FR822A.008 @ Rutgers
FR822A.007 @ Rutgers


NC samples
FR822A.020 @ Rutgers FR822A.025 @ UGA FR822A.024 @ UGA FR822A.022 @ UGA


NC5 samples
FR822A.010 @ Rutgers FR822A.031 @ Rutgers FR822A.009 @ Rutgers FR822A.012 @ Rutgers FR822A.013 @ Rutgers
FR822A.014 @ Rutgers FR822A.018 @ Rutgers FR822A.011 @ UGA FR822A.016 @ Rutgers FR822A.021 @ Rutgers
FR822A.030 @ Rutgers fr822a.023 @ UGA FR822A.017 @ Rutgers FR822A.015 @ Rutgers FR822A.029 @ Rutgers


N14 samples
FR822A.026 @ Rutgers FR822A.028 @ Rutgers FR822A.027 @ Rutgers


E-mail communications associated with this target:

  2013-09-12
  2013-09-18