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GmR137   (Gmet_1567)

database refs UniProt: Q39VC5 (Q39VC5_GEOMG)   |   NCBI gene: 3738504   |   refSeq: YP_384524.1
status NMR and X-Ray structures
structures 2K5P (NMR)   3CWI (Xray)  
properties length: 70 aa   |   mass: 7.45 kD   |   pI: 4.19   |   ext: 2980   |   organism: Geobacter metallireducens
classes PSI-2, MEGA, META Genomic, META Genomic: Gut, PSI-Biology
projects CS-Rosetta using deuterated samples     Community Outreach    
best PDB match calculating ...
informatics Prof PHD data   |   DISOPRED2 data   |   Pxs: 0.359   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures3.10.20.30 details   54285 details   PF02597 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    CATH 3.10.20.30
    SuperFamily 54285
    PFAM PF02597

GmR137

       PDB seq homology



Constructs and Most Advanced Status
   
GmR137-21.*



most recent record
7 years, 8 days ago --     purification [31.2 mg]: GmR137-21.5-DNC-ILV-Ni; purification [11.9 mg]: GmR137-21.5-DNC-ILV-GF

2007-08-29    target: GmR137
2007-11-28    construct: GmR137-21.2
2008-01-31    expression [ 5, 5 ] : GmR137-21.2-ss
2007-11-29    construct: GmR137-21.5
2008-01-03    expression [ 5, 5 ] : GmR137-21.5-SeMa
2008-01-10    purification [24.4 mg]: GmR137-21.5-SeMa-GF
2008-01-15     aggregation screening [monomer,9.38 kD,100%]
2008-01-11     GmR137.001 @ Columbia; 2 ml at 7.5 mg/mL
2008-04-21     X-Ray structure: 3CWI (PDB)
2008-01-11     GmR137.002 @ HWI; 0.45 ml at 7.5 mg/mL
2008-01-11     GmR137.003 @ Rutgers; 1.5 ml at 7.5 mg/mL
2008-01-03    expression [ 5, 5 ] : GmR137-21.5-NC5a
2008-01-10    purification [3.6 mg]: GmR137-21.5-NC5a-Ni
2008-01-16     GmR137.004 @ Rutgers; 0.012 ml at 1.6 mM
2008-01-30    HSQC [good]: GmR137.004.600MHz.20C
2008-01-16     GmR137.005 @ Rutgers; 0.246 ml at 1.6 mM
2008-01-16     GmR137.006 @ Rutgers; 1 ml at 0.387 mM
2008-01-31    expression [ 5, 5 ] : GmR137-21.5-ss
2008-01-30    expression [ 5, 5 ] : GmR137-21.5-NCa
2008-02-12    purification [37.4 mg]: GmR137-21.5-NCa-GF
2008-02-15     GmR137.007 @ Rutgers; 0.3 ml at 1.06 mM
2008-02-15     gmr137.008 @ Rutgers; 3.9 ml at 1.06 mM
2008-06-30     NMR structure: 2k5p (PDB)
2008-01-30    expression [ 5, 5 ] : GmR137-21.5-NC5b
2008-02-12    purification [28.3 mg]: GmR137-21.5-NC5b-GF
2008-02-15     GmR137.009 @ Rutgers; 0.5 ml at 1.26 mM
2008-02-15     gmr137.010 @ Rutgers; 2 ml at 1.26 mM
2008-05-30     GmR137.011 @ UGA; 0.5 ml at 1.26 mM
2010-07-07    expression [ 3, 5 ] : GmR137-21.5-DNC-ILV
2010-08-18    purification [31.2 mg]: GmR137-21.5-DNC-ILV-Ni
2010-08-18    purification [11.9 mg]: GmR137-21.5-DNC-ILV-GF
2010-08-25     GmR137.012 @ Rutgers; 0.25 ml at 0.7 mM
2010-08-26     GmR137.013 @ Buffalo; 1.7 ml at 0.7 mM
2014-11-18     GmR137.014 @ Rutgers; 0.05 ml at 0.7 mM


Available samples:

SeMet samples
GmR137.001 @ Columbia GmR137.002 @ HWI GmR137.003 @ Rutgers


Labeling not defined samples
GmR137.014 @ Rutgers GmR137.013 @ Buffalo
GmR137.012 @ Rutgers


NC samples
GmR137.007 @ Rutgers gmr137.008 @ Rutgers


NC5 samples
GmR137.009 @ Rutgers GmR137.004 @ Rutgers
GmR137.011 @ UGA gmr137.010 @ Rutgers GmR137.006 @ Rutgers GmR137.005 @ Rutgers


E-mail communications associated with this target:

  2012-11-27
  2012-11-28