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HR2978   (RBBP9)

database refs UniProt: O75884 (RBBP9_HUMAN)   |   HUGO: RBBP9   |   NCBI gene: 10741   |   refSeq: NP_006597.2
status X-Ray structure
structures 2QS9 (Xray)  
properties length: 186 aa   |   mass: 21.00 kD   |   pI: 5.79   |   ext: 41940   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T1 (Rutgers), MEGA, Tech Development: Core 50 PSI-2, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Prof PHD data   |   DISOPRED2 data   |   Pxs: 0.328   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesACT_SITE:Charge relay system (Potential) details   53474 details   3.40.50.1820 details   PF06821 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    CATH 3.40.50.1820
    SuperFamily 53474
    PFAM PF06821
    Charge relay system. {ECO:0000255} domain_number-1
    Charge relay system. {ECO:0000255} domain_number-2
    UniProt ACT_SITE:Charge relay system (Potential)
    Charge relay system. {ECO:0000255} domain_number-3

HR2978

       PDB seq homology



Constructs and Most Advanced Status
   
HR2978-_CNTRL-15.*

   
HR2978-15.*

   
HR2978-21.*

   
HR2978-14.*



most recent record
6 years, 10 days ago --     purification [100.8 mg]: HR2978-21.2-Ne-GF; purification [100.8 mg]: HR2978-21.2-Nd-GF

2006-03-15    target: HR2978
2006-09-29    construct: HR2978-15.1
2006-10-01    expression [ 0, 0 ] : HR2978-15.1-ss
2006-09-29    construct: HR2978-_CNTRL-15.1
2007-04-05    construct: HR2978-21.1
2007-04-10    expression [ 3, 4 ] : HR2978-21.1-ss
2007-04-05    construct: HR2978-21.2
2007-04-10    expression [ 3, 5 ] : HR2978-21.2-ss
2007-05-01    expression [ 3, 2 ] : HR2978-21.2-SeMa
2007-05-07    purification [26.0 mg]: HR2978-21.2-SeMa-GF
2007-05-14     aggregation screening [monomer,27.57 kD,98%]
2007-05-14     aggregation screening [monomer,27.57 kD,98%]
2007-05-16     HR2978.001 @ Columbia; 2 ml at 8.1 mg/mL
2007-05-16     HR2978.002 @ HWI; 0.45 ml at 8.1 mg/mL
2007-07-30     X-Ray structure: 2QS9 (PDB)
2007-05-01    expression [ 3, 2 ] : HR2978-21.2-SeMb
2007-05-07    purification [26.0 mg]: HR2978-21.2-SeMb-GF
2007-05-01    expression [ 2, 2 ] : HR2978-21.2-NC5b
2007-05-17    purification [16.1 mg]: HR2978-21.2-NC5b-Ni
2007-05-03    expression [ 2, 2 ] : HR2978-21.2-NC5a
2007-05-17    purification [16.1 mg]: HR2978-21.2-NC5a-Ni
2007-05-24     HR2978.003 @ Rutgers; 0.007 ml at 0.81 mM
2008-07-14    HSQC [good]: HR2978.003.600MHz.20C
2007-05-24     HR2978.004 @ Rutgers; 0.007 ml at 0.94 mM
2008-07-14    HSQC [good]: HR2978.004.600MHz.20C
2007-05-24     HR2978.005 @ Rutgers; 0.007 ml at 0.98 mM
2008-07-14    HSQC [promising]: HR2978.005.600MHz.20C
2007-05-24     HR2978.006 @ Rutgers; 0.5 ml at 0.12 mM
2007-06-14     HR2978.007 @ Rutgers; 0.007 ml at 1.1 mM
2008-07-14    HSQC [good]: HR2978.007.600MHz.25C
2007-06-14     HR2978.008 @ Rutgers; 0.007 ml at 1.43 mM
2008-07-14    HSQC [good]: HR2978.008.600MHz.25C
2007-06-14     HR2978.009 @ Rutgers; 0.007 ml at 1.4 mM
2008-07-14    HSQC [good]: HR2978.009.600MHz.25C
2007-06-06    expression [ 2, 5 ] : HR2978-21.2-NC5c
2007-06-20    purification [9.6 mg]: HR2978-21.2-NC5c-GF
2007-06-27     HR2978.010 @ Rutgers; 0.5 ml at 0.6 mM
2007-06-13    expression [ 3, 3 ] : HR2978-21.2-NC5d
2007-06-20    purification [9.6 mg]: HR2978-21.2-NC5d-GF
2007-06-13    expression [ 3, 3 ] : HR2978-21.2-NC5e
2007-06-20    purification [9.6 mg]: HR2978-21.2-NC5e-GF
2007-06-13    expression [ 3, 3 ] : HR2978-21.2-NC5f
2007-06-20    purification [9.6 mg]: HR2978-21.2-NC5f-GF
2008-07-01    expression [ 4, 5 ] : HR2978-21.2-LBa
2008-07-15    purification [15.0 mg]: HR2978-21.2-LBa-Ni
2008-07-15    purification [6.1 mg]: HR2978-21.2-LBa-GF
2008-07-22     aggregation screening [monomer,20.8 kD,99.1%]
2008-07-28     HR2978.013 @ Rutgers; 1 ml at 1 mg/mL
2008-07-22     HR2978.012 @ HWI; 0.3 ml at 11.38 mg/mL
2008-07-22     HR2978.011 @ Rutgers; 1 ml at 16.3 mg/mL
2010-09-15    expression [ 4, 4 ] : HR2978-21.2-NCa
2010-09-30    purification [22.2 mg]: HR2978-21.2-NCa-GF
2010-09-15    expression [ 4, 4 ] : HR2978-21.2-Nc
2010-09-22    purification [29.1 mg]: HR2978-21.2-Nc-GF
2010-10-05     aggregation screening [monomer,24.56 kD,93.4%]
2010-12-20     HR2978.014 @ Rutgers; 1.6 ml at 0.51 mM
2010-09-15    expression [ 4, 4 ] : HR2978-21.2-Nb
2010-09-22    purification [29.1 mg]: HR2978-21.2-Nb-GF
2010-09-15    expression [ 4, 4 ] : HR2978-21.2-Na
2010-09-22    purification [29.1 mg]: HR2978-21.2-Na-GF
2010-09-22    expression [ 4, 4 ] : HR2978-21.2-NCb
2010-09-30    purification [22.0 mg]: HR2978-21.2-NCb-GF
2010-11-08     HR2978.016 @ Rutgers; 0.4 ml at 0.42 mM
2010-11-08     HR2978.017 @ Rutgers; 2.8 ml at 0.42 mM
2010-10-05    expression [ 5, 5 ] : HR2978-21.2-LBb
2010-09-22    purification [59.3 mg]: HR2978-21.2-LBb-GF
2010-12-01     HR2978.018 @ Rutgers; 25 ml at 2.28 mg/mL
2011-02-09     HR2978.015 @ Rutgers; 1.5 ml at 2.28 mg/mL
2010-10-05    expression [ 5, 5 ] : HR2978-21.2-LBc
2010-09-22    purification [59.3 mg]: HR2978-21.2-LBc-GF
2011-03-23    expression [ 4, 3 ] : HR2978-21.2-Ne
2011-08-17    purification [100.8 mg]: HR2978-21.2-Ne-GF
2011-10-18     HR2978.019 @ Rutgers; 4 ml at 20.5 mg/mL
2011-03-23    expression [ 4, 3 ] : HR2978-21.2-Nd
2011-08-17    purification [100.8 mg]: HR2978-21.2-Nd-GF
2007-04-05    construct: HR2978-21.3
2007-04-10    expression [ 0, 0 ] : HR2978-21.3-ss
2008-06-16    construct: HR2978-14.9
2008-09-12    expression [ 4, 0 ] : HR2978-14.9-ss
2008-06-16    construct: HR2978-14.10
2008-09-12    expression [ 4, 0 ] : HR2978-14.10-ss


Available samples:

LB samples
HR2978.013 @ Rutgers HR2978.012 @ HWI HR2978.011 @ Rutgers HR2978.015 @ Rutgers HR2978.018 @ Rutgers


SeMet samples
HR2978.001 @ Columbia HR2978.002 @ HWI


Labeling not defined samples
HR2978.019 @ Rutgers


NC samples
HR2978.014 @ Rutgers HR2978.016 @ Rutgers
HR2978.017 @ Rutgers


NC5 samples
HR2978.007 @ Rutgers HR2978.008 @ Rutgers HR2978.004 @ Rutgers HR2978.006 @ Rutgers
HR2978.005 @ Rutgers HR2978.003 @ Rutgers HR2978.010 @ Rutgers HR2978.009 @ Rutgers