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HR3102A   (PSMC5)

4 targets assoicated with this target.
HR3102Bsibling(25-378)expressed
HR3102Csibling(133-396)soluble construct with good ES score
HR3102parentfermentation attempted
HR3102Dsibling(24-76)HSQC collected

database refs UniProt: P62195 (PRS8_HUMAN)   |   HUGO: PSMC5   |   NCBI gene: 5705   |   refSeq: NP_001186092.1; NP_002796.4
status NMR and X-Ray structures
structures 2KRK (NMR)   3KW6 (Xray)  
properties length: 76 aa   |   mass: 8.54 kD   |   pI: 9.02   |   ext: 1490   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T1 (Rutgers), Community Outreach, BIG: Rutgers, MEGA, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects CS-Rosetta using deuterated samples     Community Outreach     HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures52540 details   1.10.8.60 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR3102A).
Display constructs associated with this target (HR3102A) and all other associated targets (HR3102, HR3102C, HR3102D, HR3102B).


Domain and UniProt Features
    BIG BIG_1089
    SuperFamily 52540
    CATH 3.40.50.300
    PFAM PF00004
    ATP. {ECO:0000255} domain_number-1
    UniProt NP_BIND:ATP (Potential)


NESG targets
    HR3102D
    HR3102B
    HR3102C
    HR3102A

HR3102

       PDB seq homology



Constructs and Most Advanced Status
   
HR3102-15.*

   
HR3102-15-406-15.*

   
HR3102B-15-393-15.*

   
HR3102D-15-91-15.*

   
HR3102D-36-91-15.*

   
HR3102B-36-393-15.*

   
HR3102C-126-406-15.*

   
HR3102-180-377-14.*

   
HR3102-262-406-14.*

   
HR3102A-315-400-14.*

   
HR3102A-320-395-14.*



most recent record
3 years, 25 days ago --     HSQC [not scored]

2009-05-19    target: HR3102A
PLIMS data     Construct creation records
PLIMS data     Construct transfection records
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE analytical scores
2009-06-18    construct: HR3102A-320-395-14.5
2009-07-15    expression [ 3, 5 ] : HR3102A-320-395-14.5-ss
2009-07-21    expression [ 5, 5 ] : HR3102A-320-395-14.5-NC5a
2009-09-08    purification [78.8 mg]: HR3102A-320-395-14.5-NC5a-GF
2009-09-28     HR3102A.009 @ Rutgers; 0.04 ml at 0.937 mM
2009-10-24    HSQC [good]: HR3102A.009.600MHz.20C
2009-09-14     HR3102A.010 @ Rutgers; 4.3925 ml at 0.937 mM
2013-04-22     HR3102A.031 @ Rutgers; 0.05 ml at 0.4685 mM
2014-04-28    HSQC [not scored]: HR3102A.031.600MHz.20C
2013-04-22     HR3102A.030 @ Rutgers; 0.05 ml at 0.4685 mM
2014-04-29    HSQC [not scored]: HR3102A.030.600MHz.20C
2013-04-22     HR3102A.029 @ Rutgers; 0.05 ml at 0.4685 mM
2014-04-28    HSQC [not scored]: HR3102A.029.600MHz.20C
2013-05-20     HR3102A.032 @ Rutgers; 0.05 ml at 0.04685 mM
2013-05-20     HR3102A.033 @ Rutgers; 0.05 ml at 0.04685 mM
2013-05-20     HR3102A.034 @ Rutgers; 0.05 ml at 0.04685 mM
2014-01-09     HR3102A.035 @ Rutgers; 0.5 ml at 0.04685 mM
2009-07-21    expression [ 5, 5 ] : HR3102A-320-395-14.5-NC5b
2009-11-09    purification [104.0 mg]: HR3102A-320-395-14.5-NC5b-GF
2009-12-03     HR3102A.020 @ Rutgers; 0.5 ml at 0.89 mM
2009-11-11     HR3102A.016 @ Rutgers; 1 ml at 0.89 mM
2009-11-11     HR3102A.017 @ Rutgers; 8 ml at 0.89 mM
2009-12-03     HR3102A.021 @ Rutgers; 0.5 ml at 0.89 mM
2010-09-16     HR3102A.025 @ Rutgers; 0.5 ml at 0.8 mM
2010-09-16     HR3102A.026 @ Rutgers; 0.5 ml at 0.8 mM
2009-08-04    expression [ 3, 5 ] : HR3102A-320-395-14.5-SeMb
2009-08-24    purification [75.6 mg]: HR3102A-320-395-14.5-SeMb-GF
2009-09-01     aggregation screening [monomer,10.4 kD,51.6%|monomer,11.5 kD,37.4%|monomer,12.0 kD,8.4%]
2009-08-28     HR3102A.004 @ Columbia; 2 ml at 9.96 mg/mL
2009-11-30     X-Ray structure: 3KW6 (PDB)
2009-08-28     HR3102A.005 @ HWI; 0.45 ml at 9.96 mg/mL
2009-08-28     hr3102a.006 @ Rutgers; 3 ml at 9.96 mg/mL
2010-07-19     HR3102A.024 @ Columbia; 2 ml at 9.05 mg/mL
2009-08-04    expression [ 3, 5 ] : HR3102A-320-395-14.5-SeMa
2009-08-24    purification [75.6 mg]: HR3102A-320-395-14.5-SeMa-GF
2009-09-09    expression [ 5, 5 ] : HR3102A-320-395-14.5-TB_midi_a
2009-11-04    expression [ 5, 5 ] : HR3102A-320-395-14.5-NCa
2009-11-09    purification [85.5 mg]: HR3102A-320-395-14.5-NCa-GF
2009-11-11     HR3102A.014 @ Rutgers; 0.3 ml at 0.82 mM
2009-12-18     NMR structure: 2krk (PDB)
2009-11-11     hr3102a.015 @ Rutgers; 7.5 ml at 0.82 mM
2009-11-04    expression [ 5, 5 ] : HR3102A-320-395-14.5-NC5c
2009-11-09    purification [104.0 mg]: HR3102A-320-395-14.5-NC5c-GF
2010-06-16    expression [ 3, 5 ] : HR3102A-320-395-14.5-DNC-ILV
2010-06-29    purification [7.2 mg]: HR3102A-320-395-14.5-DNC-ILV-GF
2010-07-01     HR3102A.022 @ Rutgers; 0.1 ml at 0.92 mM
2010-07-01     hr3102a.023 @ Rutgers; 0.05 ml at 0.92 mM
2012-11-26     HR3102A.027 @ Buffalo; 0.3 ml at 0.92 mM
2013-03-04     HR3102A.028 @ Rutgers; 0.04 ml at 0.92 mM
2014-11-18     HR3102A.036 @ Rutgers; 0.05 ml at 0.92 mM
2010-06-29    purification [9.3 mg]: HR3102A-320-395-14.5-DNC-ILV-Ni
2009-06-18    construct: HR3102A-320-395-14.6
2009-07-15    expression [ 3, 5 ] : HR3102A-320-395-14.6-ss
2009-06-16    construct: HR3102A-315-400-14.1
2009-07-15    expression [ 3, 4 ] : HR3102A-315-400-14.1-ss
2009-09-09    expression [ 4, 5 ] : HR3102A-315-400-14.1-TB_midi_a
2009-10-27    expression [ 4, 5 ] : HR3102A-315-400-14.1-SeMb
2009-11-03    purification [69.7 mg]: HR3102A-315-400-14.1-SeMb-GF
2009-11-09     HR3102A.011 @ Rutgers; 2 ml at 7.27 mg/mL
2009-11-09     HR3102A.012 @ Rutgers; 0.45 ml at 7.27 mg/mL
2009-11-09     HR3102A.013 @ Rutgers; 5 ml at 7.27 mg/mL
2009-10-27    expression [ 4, 5 ] : HR3102A-315-400-14.1-SeMa
2009-11-03    purification [69.7 mg]: HR3102A-315-400-14.1-SeMa-GF
2009-10-27    expression [ 3, 5 ] : HR3102A-315-400-14.1-NC5b
2009-10-27    expression [ 3, 5 ] : HR3102A-315-400-14.1-NC5a
2009-11-05    purification [3.6 mg]: HR3102A-315-400-14.1-NC5a-Ni
2009-11-06    purification [34.9 mg]: HR3102A-315-400-14.1-NC5a-GF
2009-11-16     HR3102A.019 @ Rutgers; 0.4 ml at 0.521 mM
2009-11-16     HR3102A.018 @ Rutgers; 0.04 ml at 0.521 mM
2010-01-04    HSQC [promising]: HR3102A.018.600MHz.20C
2009-06-16    construct: HR3102A-315-400-14.2
2009-07-15    expression [ 3, 5 ] : HR3102A-315-400-14.2-ss
2009-07-21    expression [ 5, 5 ] : HR3102A-315-400-14.2-SeMa
2009-07-27    purification [38.7 mg]: HR3102A-315-400-14.2-SeMa-GF
2009-08-04     aggregation screening [Aggregated,187.7 kD,208.7%]
2009-07-31     HR3102A.002 @ HWI; 0.45 ml at 9.213 mg/mL
2009-07-31     HR3102A.001 @ Columbia; 2 ml at 9.213 mg/mL
2009-07-31     HR3102A.003 @ Rutgers; 2.5 ml at 9.213 mg/mL
2009-07-21    expression [ 5, 5 ] : HR3102A-315-400-14.2-NC5b
2009-07-21    expression [ 5, 5 ] : HR3102A-315-400-14.2-SeMb
2009-07-27    purification [38.7 mg]: HR3102A-315-400-14.2-SeMb-GF
2009-07-21    expression [ 5, 5 ] : HR3102A-315-400-14.2-NC5a
2009-09-08    purification [79.7 mg]: HR3102A-315-400-14.2-NC5a-GF
2009-09-28     HR3102A.007 @ Rutgers; 0.04 ml at 0.629 mM
2009-10-24    HSQC [good]: HR3102A.007.600MHz.20C
2009-09-14     HR3102A.008 @ Rutgers; 4.25 ml at 0.629 mM


Available samples:

SeMet samples
HR3102A.013 @ Rutgers HR3102A.024 @ Columbia HR3102A.005 @ HWI HR3102A.003 @ Rutgers HR3102A.012 @ Rutgers
HR3102A.001 @ Columbia hr3102a.006 @ Rutgers HR3102A.004 @ Columbia HR3102A.002 @ HWI HR3102A.011 @ Rutgers


Labeling not defined samples
HR3102A.027 @ Buffalo HR3102A.028 @ Rutgers HR3102A.022 @ Rutgers HR3102A.036 @ Rutgers hr3102a.023 @ Rutgers


NC samples
HR3102A.014 @ Rutgers hr3102a.015 @ Rutgers


NC5 samples
HR3102A.010 @ Rutgers HR3102A.031 @ Rutgers HR3102A.017 @ Rutgers
HR3102A.020 @ Rutgers HR3102A.008 @ Rutgers HR3102A.009 @ Rutgers HR3102A.032 @ Rutgers HR3102A.035 @ Rutgers
HR3102A.019 @ Rutgers HR3102A.018 @ Rutgers HR3102A.025 @ Rutgers HR3102A.016 @ Rutgers HR3102A.026 @ Rutgers
HR3102A.021 @ Rutgers HR3102A.030 @ Rutgers HR3102A.034 @ Rutgers HR3102A.033 @ Rutgers HR3102A.007 @ Rutgers
HR3102A.029 @ Rutgers


E-mail communications associated with this target:

  2012-11-27
  2012-11-28