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HR3504C   (TEC)

4 targets assoicated with this target.
HR3504parentselected
HR3504Bsibling(247-631)selected
HR3504Asibling(1-103)selected
HR3504Dsibling(353-622)expressed

database refs UniProt: P42680 (TEC_HUMAN)   |   HUGO: TEC   |   NCBI gene: 7006   |   refSeq: NP_003206.2
status NMR structure and crystal hits
structures 2LUL (NMR)  
properties length: 149 aa   |   mass: 17.68 kD   |   pI: 9.43   |   ext: 24410   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T2 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:Btk-type details   DOMAIN:PH details   METAL:Zinc (By similarity) details   PF00779 details   50729 details   2.30.29.30 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR3504C).
Display constructs associated with this target (HR3504C) and all other associated targets (HR3504, HR3504D, HR3504B, HR3504A).


Domain and UniProt Features
    CATH 2.30.29.30
    UniProt DOMAIN:PH
    PH. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:Btk-type
    Zinc finger - Btk-type. {ECO:0000255|PROSITE- domain_number-1
    Zinc. {ECO:0000250} domain_number-1
    Zinc. {ECO:0000250} domain_number-2
    Zinc. {ECO:0000250} domain_number-3
    UniProt METAL:Zinc (By similarity)
    Zinc. {ECO:0000250} domain_number-4
    SH3. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3
    UniProt DOMAIN:SH2
    SH2. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    PFAM PF07714
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt ACT_SITE:Proton acceptor (By similarity)
    Proton acceptor. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR3504A
    HR3504C
    HR3504B
    HR3504D

HR3504

       PDB seq homology



Constructs and Most Advanced Status
   
HR3504C-1-154-14.*

   
HR3504C-1-154-AvMBPB2.*

   
HR3504C-1-149-14.*

   
HR3504C-1-154-AvSUMO.*

   
HR3504C-1-154-Av6HT.*

   
HR3504C-1-154-SGCAv.*

   
HR3504D-242-631-15.*

   
HR3504D-246-624-15.*

   
HR3504D-246-631-15.*

   
HR3504D-348-631-15.*

   
HR3504D-353-631-15.*



most recent record
4 years, 2 days ago --     expression [4, 5]: HR3504C-1-154-AvSUMO.24-ss; expression [4, 5]: HR3504C-1-154-AvSUMO.25-ss

2009-06-22    target: HR3504C
2009-08-03    construct: HR3504C-1-154-14.1
2009-08-13    expression [ 5, 5 ] : HR3504C-1-154-14.1-ss
2009-08-18    expression [ 4, 5 ] : HR3504C-1-154-14.1-SeMa
2009-08-25    purification [54.6 mg]: HR3504C-1-154-14.1-SeMa-GF
2009-09-02     aggregation screening [monomer,23.4 kD,84.0%]
2009-08-31     HR3504C.001 @ Columbia; 2 ml at 5.503 mg/mL
2009-08-31     HR3504C.002 @ HWI; 0.45 ml at 5.503 mg/mL
2009-08-31     HR3504C.003 @ Rutgers; 3.5 ml at 5.503 mg/mL
2009-08-18    expression [ 4, 5 ] : HR3504C-1-154-14.1-NC5a
2009-09-23    purification [6.2 mg]: HR3504C-1-154-14.1-NC5a-GF
2009-09-30     HR3504C.006 @ Rutgers; 0.04 ml at 0.665 mM
2009-10-24    HSQC [good]: HR3504C.006.600MHz.20C
2009-09-30     HR3504C.007 @ Rutgers; 0.25 ml at 0.665 mM
2011-06-21    expression [ 5, 5 ] : HR3504C-1-154-14.1-NCa
2011-06-28    purification [116.0 mg]: HR3504C-1-154-14.1-NCa-Ni
2011-06-29    purification [50.0 mg]: HR3504C-1-154-14.1-NCa-GF
2011-07-02     hr3504c.023 @ Rutgers; 0.3 ml at 0.614 mM
2011-07-02     HR3504C.024 @ Rutgers; 2.5 ml at 0.808 mM
2012-06-15     NMR structure: 2lul (PDB)
2011-06-21    expression [ 5, 5 ] : HR3504C-1-154-14.1-NC5b
2011-06-28    purification [119.0 mg]: HR3504C-1-154-14.1-NC5b-Ni
2011-06-29    purification [49.2 mg]: HR3504C-1-154-14.1-NC5b-GF
2011-07-02     HR3504C.025 @ Rutgers; 0.5 ml at 0.828 mM
2011-07-02     HR3504C.026 @ Rutgers; 2 ml at 0.828 mM
2012-01-27     HR3504C.027 @ UGA; 0.5 ml at 0.52 mM
2009-08-03    construct: HR3504C-1-154-14.2
2009-08-13    expression [ 4, 5 ] : HR3504C-1-154-14.2-ss
2009-08-03    construct: HR3504C-1-149-14.3
2009-08-13    expression [ 4, 5 ] : HR3504C-1-149-14.3-ss
2009-08-04    construct: HR3504C-1-149-14.8
2009-08-13    expression [ 4, 5 ] : HR3504C-1-149-14.8-ss
2009-08-18    expression [ 4, 5 ] : HR3504C-1-149-14.8-NC5a
2009-09-23    purification [6.5 mg]: HR3504C-1-149-14.8-NC5a-GF
2009-09-30     HR3504C.004 @ Rutgers; 0.04 ml at 0.551 mM
2009-10-24    HSQC [good]: HR3504C.004.600MHz.20C
2009-09-30     HR3504C.005 @ Rutgers; 0.3 ml at 0.551 mM
2009-09-08    expression [ 3, 4 ] : HR3504C-1-149-14.8-SeMa
2009-12-02    purification [16.9 mg]: HR3504C-1-149-14.8-SeMa-GF
2009-12-08     aggregation screening [heptamer,127.5 kD,89.9%]
2009-12-08     HR3504C.008 @ Rutgers; 2 ml at 10.04 mg/mL
2009-12-08     HR3504C.009 @ HWI; 0.45 ml at 10.04 mg/mL
2011-02-23    expression [ 5, 5 ] : HR3504C-1-149-14.8-NC5b
2011-05-26    purification [64.6 mg]: HR3504C-1-149-14.8-NC5b-Ni
2011-06-07     HR3504C.010 @ Rutgers; 2.52 ml at 0.5 mM
2011-06-08     HR3504C.018 @ Rutgers; 0.04 ml at 1.32042 mM
2011-06-21    HSQC [good]: HR3504C.018.600MHz.25C
2011-06-08     HR3504C.019 @ Rutgers; 0.04 ml at 1.59058 mM
2011-06-21    HSQC [good]: HR3504C.019.600MHz.25C
2011-06-08     HR3504C.017 @ Rutgers; 0.04 ml at 1.31187 mM
2011-06-22    HSQC [good]: HR3504C.017.600MHz.25C
2011-06-08     HR3504C.021 @ Rutgers; 0.04 ml at 1.25569 mM
2011-06-21    HSQC [poor]: HR3504C.021.600MHz.25C
2011-06-08     HR3504C.011 @ Rutgers; 0.04 ml at 1.26099 mM
2011-06-21    HSQC [promising]: HR3504C.011.600MHz.25C
2011-06-08     HR3504C.016 @ Rutgers; 0.04 ml at 1.27461 mM
2011-06-21    HSQC [promising]: HR3504C.016.600MHz.25C
2011-06-08     HR3504C.013 @ Rutgers; 0.04 ml at 1.28202 mM
2011-06-22    HSQC [good]: HR3504C.013.600MHz.25C
2011-06-08     HR3504C.020 @ Rutgers; 0.04 ml at 1.23839 mM
2011-06-21    HSQC [promising]: HR3504C.020.600MHz.25C
2011-06-08     HR3504C.014 @ Rutgers; 0.04 ml at 1.26536 mM
2011-06-22    HSQC [promising]: HR3504C.014.600MHz.25C
2011-06-08     HR3504C.022 @ Rutgers; 0.04 ml at 1.58944 mM
2011-06-21    HSQC [promising]: HR3504C.022.600MHz.25C
2011-06-08     HR3504C.012 @ Rutgers; 0.04 ml at 1.52478 mM
2011-06-22    HSQC [promising]: HR3504C.012.600MHz.25C
2011-06-08     HR3504C.015 @ Rutgers; 0.04 ml at 1.50698 mM
2011-06-21    HSQC [good]: HR3504C.015.600MHz.25C
2012-11-19    construct: HR3504C-1-154-AvMBPB2.11
2013-01-30     HR3504C.028 @ Rutgers; construct sample
2012-12-11    expression [ 5, 5, not tested ]: HR3504C-1-154-AvMBPB2.11-ss
2013-02-05    expression [ 5, 5, not tested ]: HR3504C-1-154-AVMBPB2.11-LBa
2013-02-21    purification [38.4 mg]: HR3504C-1-154-AVMBPB2.11-LBa-GF
2013-04-17     aggregation screening [monomer,79.4 kD,83.7%|aggregated,1512 kD,5.2%]
2013-02-25     HR3504C.030 @ Rutgers; 14.9 ml at 2.5 mg/mL
2013-02-25     HR3504C.031 @ RAN - Chicago; 1.5 ml at 0.9 mg/mL
2013-04-16     HR3504C.032 @ HWI; 0.1 ml at 2.5 mg/mL
2012-11-19    construct: HR3504C-1-154-SGCAv.13
2012-12-11    expression [ 3, 4 ] : HR3504C-1-154-SGCAv.13-ss
2012-11-19    construct: HR3504C-1-154-SGCAv.14
2012-12-11    expression [ 3, 4 ] : HR3504C-1-154-SGCAv.14-ss
2012-11-20    construct: HR3504C-1-154-Av6HT.19
2012-12-11    expression [ 3, 4, not tested ]: HR3504C-1-154-Av6HT.19-ss
2012-11-19    construct: HR3504C-1-154-AvMBPB2.12
2013-01-30     HR3504C.029 @ Rutgers; construct sample
2012-12-11    expression [ 5, 5, not tested ]: HR3504C-1-154-AvMBPB2.12-ss
2012-11-19    construct: HR3504C-1-154-Av6HT.15
2012-12-11    expression [ 3, 1, not tested ]: HR3504C-1-154-Av6HT.15-ss
2013-09-03    construct: HR3504C-1-154-AvSUMO.24
2013-09-23    expression [ 4, 5, not tested ]: HR3504C-1-154-AvSUMO.24-ss
2013-09-03    construct: HR3504C-1-154-AvSUMO.25
2013-09-23    expression [ 4, 5, not tested ]: HR3504C-1-154-AvSUMO.25-ss


Available samples:

LB samples
HR3504C.032 @ HWI HR3504C.030 @ Rutgers HR3504C.031 @ RAN - Chicago


SeMet samples
HR3504C.008 @ Rutgers HR3504C.009 @ HWI
HR3504C.002 @ HWI HR3504C.003 @ Rutgers HR3504C.001 @ Columbia


NC samples
hr3504c.023 @ Rutgers HR3504C.024 @ Rutgers


NC5 samples
HR3504C.026 @ Rutgers HR3504C.017 @ Rutgers HR3504C.012 @ Rutgers HR3504C.005 @ Rutgers HR3504C.015 @ Rutgers
HR3504C.019 @ Rutgers HR3504C.011 @ Rutgers HR3504C.016 @ Rutgers HR3504C.006 @ Rutgers HR3504C.027 @ UGA
HR3504C.025 @ Rutgers HR3504C.021 @ Rutgers HR3504C.013 @ Rutgers HR3504C.007 @ Rutgers HR3504C.010 @ Rutgers
HR3504C.022 @ Rutgers HR3504C.020 @ Rutgers HR3504C.018 @ Rutgers HR3504C.004 @ Rutgers HR3504C.014 @ Rutgers


E-mail communications associated with this target:

  2013-01-31
  2013-02-19
  2013-04-18
  2013-04-18
  2013-04-18
  2013-04-18
  2013-04-24
  2013-04-24
  2013-05-02
  2013-05-02