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HR3646E   (ITSN1)

7 targets assoicated with this target.
HR3646parentselected
HR3646Asibling(1-181)selected
HR3646Dsibling(1582-1721)selected
HR3646Csibling(701-1228)selected
HR3646Bsibling(193-293)selected
HR3646Fsibling(1589-1721)expression tested
HR3646Gsibling(1153-1228)good HSQC collected and crystal hits

database refs UniProt: Q15811 (ITSN1_HUMAN)   |   HUGO: ITSN1   |   NCBI gene: 6453   |   refSeq: NP_001001132.1; NP_003015.2
status NMR and X-Ray structures
structures 2KHN (NMR)   3FIA (Xray)  
properties length: 111 aa   |   mass: 12.37 kD   |   pI: 6.71   |   ext: 12490   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T4 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Prof PHD data   |   Pxs: 0.312   |   Zn binding sites: Analysis not performed.
domain signaturesCA_BIND:1 (Potential) details   1.10.238.10 details   DOMAIN:EH 1 details   47473 details   DOMAIN:EF-hand 1 details   PF12763 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR3646E).
Display constructs associated with this target (HR3646E) and all other associated targets (HR3646, HR3646A, HR3646G, HR3646D, HR3646F, HR3646C, HR3646B).


Domain and UniProt Features
    UniProt DOMAIN:EH 1
    EH 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:EF-hand 1
    EF-hand 1. {ECO:0000255|PROSITE- domain_number-1
    Ca binding - 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt CA_BIND:1 (Potential)
    CATH 1.10.238.10
    SuperFamily 47473
    PFAM PF12763
    UniProt DOMAIN:EH 2
    EH 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:EF-hand 2
    EF-hand 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt CA_BIND:2 (Potential)
    Ca binding - 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 1
    SH3 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 2
    SH3 2. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 50044
    CATH 2.30.30.40
    UniProt DOMAIN:SH3 3
    SH3 3. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF07653
    CATH 2.30.30.40
    SuperFamily 50044
    SH3 4. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 4
    PFAM PF07653
    SuperFamily 50044
    CATH 2.30.30.40
    SH3 5. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 5
    PFAM PF00018
    SuperFamily 48065
    CATH 1.20.900.10
    DH. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:DH
    PFAM PF00621
    PH. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:PH
    C2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:C2


NESG targets
    HR3646A
    HR3646E
    HR3646B
    HR3646C
    HR3646G
    HR3646D
    HR3646F

HR3646

       PDB seq homology



Constructs and Most Advanced Status
   
HR3646E-Av6HT.*

   
HR3646E-14.*

   
HR3646E-AvSUMO.*

   
HR3646E-AvMBPB2.*

   
HR3646E-SGCAv.*

   
HR3646E-15.*

   
HR3646G-1148-1233-14.*

   
HR3646G-1153-1228-14.*

   
HR3646F-1584-1721-14.*

   
HR3646F-1589-1721-14.*



most recent record
3 years, 27 days ago --     expression [4, 4]: HR3646E-AvMBPB2.21-ss; expression [4, 4]: HR3646E-AvMBPB2.22-ss

2007-05-23    target: HR3646E
PLIMS data     Construct creation records
PLIMS data     Construct transfection records
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE analytical scores
2007-12-14    construct: HR3646E-15.1
2008-01-24    expression [ 3, 2 ] : HR3646E-15.1-ss
2008-03-13    construct: HR3646E-14.3
2013-09-10    construct: HR3646E-AvSUMO.19
2013-09-23    expression [ 5, 4, not tested ]: HR3646E-AvSUMO.19-ss
2008-08-28    construct: HR3646E-14.7
2008-09-26    expression [ 4, 4 ] : HR3646E-14.7-ss
2008-08-28    construct: HR3646E-14.8
2008-09-26    expression [ 4, 4 ] : HR3646E-14.8-ss
2008-10-07    expression [ 3, 3 ] : HR3646E-14.8-SeMa
2008-10-20    purification [20.5 mg]: HR3646E-14.8-SeMa-GF
2008-10-28     aggregation screening [monomer,13.46 kD,91.8%]
2008-10-21     HR3646E.001 @ Columbia; 1.7 ml at 8.29 mg/mL
2008-12-11     X-Ray structure: 3FIA (PDB)
2008-10-21     HR3646E.002 @ HWI; 0.45 ml at 8.29 mg/mL
2008-10-07    expression [ 3, 3 ] : HR3646E-14.8-NC5a
2008-10-24    purification [16.6 mg]: HR3646E-14.8-NC5a-GF
2008-11-06     HR3646E.003 @ Rutgers; 0.012 ml at 0.73 mM
2008-11-24    HSQC [good]: HR3646E.003.600MHz.20C
2009-01-08     hr3646e.004 @ Rutgers; 0.2 ml at 0.736 mM
2008-12-02    expression [ 2, 4 ] : HR3646E-14.8-NC5b
2008-12-10    expression [ 3, 4 ] : HR3646E-14.8-NCa
2008-12-15    purification [17.2 mg]: HR3646E-14.8-NCa-GF
2008-12-18     HR3646E.005 @ Buffalo; 0.3 ml at 0.87 mM
2009-05-12    HSQC [good]: HR3646E.005.500MHz.20C
2009-04-09     NMR structure: 2KHN (PDB)
2008-12-18     hr3646e.006 @ Rutgers; 0.6 ml at 0.87 mM
2008-12-16    expression [ 3, 5 ] : HR3646E-14.8-NC5c
2009-01-16    purification [10.9 mg]: HR3646E-14.8-NC5c-GF
2009-01-16     HR3646E.007 @ Buffalo; 0.5 ml at 0.81 mM
2009-01-16     hr3646e.008 @ UGA; 0.35 ml at 0.81 mM
2012-11-19    construct: HR3646E-Av6HT.13
2012-12-11    expression [ 5, 4, not tested ]: HR3646E-Av6HT.13-ss
2012-11-20    construct: HR3646E-AvMBPB2.18
2012-12-11    expression [ 0, 0, not tested ]: HR3646E-AvMBPB2.18-ss
2012-11-19    construct: HR3646E-SGCAv.11
2012-12-11    expression [ 3, 3 ] : HR3646E-SGCAv.11-ss
2012-11-19    construct: HR3646E-AvMBPB2.10
2013-01-30     HR3646E.009 @ Rutgers; construct sample
2012-12-11    expression [ 3, 4, not tested ]: HR3646E-AvMBPB2.10-ss
2013-02-05    expression [ 4, 5, not tested ]: HR3646E-AVMBPB2.10-LBa
2013-02-21    purification [18.2 mg]: HR3646E-AVMBPB2.10-LBa-GF
2013-02-25     HR3646E.010 @ Rutgers; 8 ml at 1.3 mg/mL
2013-02-25     HR3646E.011 @ Rutgers; 1.5 ml at 1.55 mg/mL
2012-11-19    construct: HR3646E-SGCAv.12
2012-12-11    expression [ 3, 4 ] : HR3646E-SGCAv.12-ss
2012-11-19    construct: HR3646E-Av6HT.14
2012-12-11    expression [ 5, 0, not tested ]: HR3646E-Av6HT.14-ss
2013-09-10    construct: HR3646E-AvSUMO.20
2013-09-23    expression [ 5, 4, not tested ]: HR3646E-AvSUMO.20-ss
2014-01-09    construct: HR3646E-AvMBPB2.21
2014-01-31    expression [ 4, 4, not tested ]: HR3646E-AvMBPB2.21-ss
2014-01-09    construct: HR3646E-AvMBPB2.22
2014-01-31    expression [ 4, 4, not tested ]: HR3646E-AvMBPB2.22-ss


Available samples:

LB samples
HR3646E.010 @ Rutgers HR3646E.011 @ Rutgers


SeMet samples
HR3646E.001 @ Columbia HR3646E.002 @ HWI


NC samples
hr3646e.006 @ Rutgers
HR3646E.005 @ Buffalo


NC5 samples
hr3646e.004 @ Rutgers hr3646e.008 @ UGA HR3646E.003 @ Rutgers HR3646E.007 @ Buffalo


E-mail communications associated with this target:

  2013-01-31
  2013-02-19