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HR3646G   (ITSN1)

7 targets assoicated with this target.
HR3646parentselected
HR3646Asibling(1-181)selected
HR3646Dsibling(1582-1721)selected
HR3646Csibling(701-1228)selected
HR3646Bsibling(193-293)selected
HR3646Fsibling(1589-1721)expression tested
HR3646Esibling(1-111)NMR and X-Ray structures

database refs UniProt: Q15811 (ITSN1_HUMAN)   |   HUGO: ITSN1   |   NCBI gene: 6453   |   refSeq: NP_001001132.1; NP_003015.2
status good HSQC collected and crystal hits    work stopped (2010-06-30; End of PSI-2)
properties length: 76 aa   |   mass: 8.58 kD   |   pI: 4.11   |   ext: 20970   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T2 (Rutgers), Biomedical: Cancer BioNet, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: Analysis not performed.
domain signaturesPF00018 details   50044 details   2.30.30.40 details   DOMAIN:SH3 5 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR3646G).
Display constructs associated with this target (HR3646G) and all other associated targets (HR3646, HR3646A, HR3646E, HR3646D, HR3646F, HR3646C, HR3646B).


Domain and UniProt Features
    UniProt DOMAIN:EH 1
    EH 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:EF-hand 1
    EF-hand 1. {ECO:0000255|PROSITE- domain_number-1
    Ca binding - 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt CA_BIND:1 (Potential)
    CATH 1.10.238.10
    SuperFamily 47473
    PFAM PF12763
    UniProt DOMAIN:EH 2
    EH 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:EF-hand 2
    EF-hand 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt CA_BIND:2 (Potential)
    Ca binding - 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 1
    SH3 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 2
    SH3 2. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 50044
    CATH 2.30.30.40
    UniProt DOMAIN:SH3 3
    SH3 3. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF07653
    CATH 2.30.30.40
    SuperFamily 50044
    SH3 4. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 4
    PFAM PF07653
    SuperFamily 50044
    CATH 2.30.30.40
    SH3 5. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3 5
    PFAM PF00018
    SuperFamily 48065
    CATH 1.20.900.10
    DH. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:DH
    PFAM PF00621
    PH. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:PH
    C2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:C2


NESG targets
    HR3646A
    HR3646E
    HR3646B
    HR3646C
    HR3646G
    HR3646D
    HR3646F

HR3646

       PDB seq homology



Constructs and Most Advanced Status
   
HR3646E-Av6HT.*

   
HR3646E-14.*

   
HR3646E-AVMBPB2.*

   
HR3646E-AvMBPB2.*

   
HR3646E-AvSUMO.*

   
HR3646E-SGCAv.*

   
HR3646E-15.*

   
HR3646G-1148-1233-14.*

   
HR3646G-1153-1228-14.*

   
HR3646F-1584-1721-14.*

   
HR3646F-1589-1721-14.*



most recent record
6 years, 19 days ago --     HSQC [good]

2010-03-09    target: HR3646G     work stopped (2010-06-30; End of PSI-2)
2010-04-28    construct: HR3646G-1153-1228-14.1     work stopped (2010-06-30; End of PSI-2)
2010-06-14    expression [ 5, 5 ] : HR3646G-1153-1228-14.1-ss
2010-06-22    expression [ 3, 5 ] : HR3646G-1153-1228-14.1-SeMa
2011-02-01    purification [28.8 mg]: HR3646G-1153-1228-14.1-SeMa-GF
2011-02-08     aggregation screening [monomer,10.24 kD,69.7%|monomer,11.17 kD,17.55%]
2011-02-09     hr3646g.001 @ Rutgers; 2 ml at 7.09 mg/mL
2011-02-09     HR3646G.002 @ HWI; 0.45 ml at 7.09 mg/mL
2011-02-09     HR3646G.003 @ Rutgers; 0.5 ml at 7.09 mg/mL
2011-02-08    expression [ 4, 5 ] : HR3646G-1153-1228-14.1-SeM-R1
2011-02-15    purification [39.2 mg]: HR3646G-1153-1228-14.1-SeM-R1-GF
2011-02-23     aggregation screening [monomer,10.34 kD,55.8%|monomer,11.61 kD,21.55%|monomer,12.12 kD,11.42%]
2011-02-18     hr3646g.004 @ Rutgers; 2 ml at 7.1 mg/mL
2011-02-18     HR3646G.005 @ HWI; 0.45 ml at 7.1 mg/mL
2011-02-18     HR3646G.006 @ Rutgers; 1 ml at 7.1 mg/mL
2011-02-22    expression [ 4, 5 ] : HR3646G-1153-1228-14.1-NC5a
2011-03-03    purification [26.4 mg]: HR3646G-1153-1228-14.1-NC5a-Ni
2011-03-04    purification [22.2 mg]: HR3646G-1153-1228-14.1-NC5a-GF
2011-03-10     HR3646G.009 @ Rutgers; 0.04 ml at 0.4 mM
2011-05-09    HSQC [good]: HR3646G.009.600MHz.20C
2011-03-13     HR3646G.010 @ Rutgers; 1.5 ml at 0.4 mM
2010-04-28    construct: HR3646G-1153-1228-14.2     work stopped (2010-06-30; End of PSI-2)
2010-06-14    expression [ 4, 3 ] : HR3646G-1153-1228-14.2-ss
2010-04-28    construct: HR3646G-1148-1233-14.6     work stopped (2010-06-30; End of PSI-2)
2010-06-14    expression [ 4, 5 ] : HR3646G-1148-1233-14.6-ss
2010-06-30    expression [ 2, 5 ] : HR3646G-1148-1233-14.6-SeMa
2010-08-11    expression [ 1, 5 ] : HR3646G-1148-1233-14.6-NC5a
2011-02-08    expression [ 3, 5 ] : HR3646G-1148-1233-14.6-SeM-R1
2011-02-15    purification [16.8 mg]: HR3646G-1148-1233-14.6-SeM-R1-GF
2011-02-23     aggregation screening [monomer,13.53 kD,58.0%|monomer,12.11 kD,38.12%]
2011-02-18     hr3646g.007 @ Rutgers; 1.1 ml at 7.4 mg/mL
2011-02-18     HR3646G.008 @ HWI; 0.45 ml at 7.4 mg/mL
2010-04-28    construct: HR3646G-1148-1233-14.7     work stopped (2010-06-30; End of PSI-2)
2010-06-14    expression [ 4, 4 ] : HR3646G-1148-1233-14.7-ss


Available samples:

SeMet samples
hr3646g.001 @ Rutgers HR3646G.005 @ HWI HR3646G.003 @ Rutgers HR3646G.008 @ HWI HR3646G.006 @ Rutgers
hr3646g.004 @ Rutgers hr3646g.007 @ Rutgers HR3646G.002 @ HWI


NC5 samples
HR3646G.010 @ Rutgers HR3646G.009 @ Rutgers