JavaScript Menu Courtesy of Milonic.com
[nucleotide sequence]     [protein sequence]     [ncbi BLAST]     [add construct]     [add ligand annotation]

HR4394C   (ARID3A)

3 targets assoicated with this target.
HR4394parentselected
HR4394Asibling(214-421)selected
HR4394Bsibling(453-537)selected

database refs UniProt: Q99856 (ARI3A_HUMAN)   |   HUGO: ARID3A   |   NCBI gene: 1820   |   refSeq: NP_005215.1
status NMR structure
structures 2KK0 (NMR)  
properties length: 134 aa   |   mass: 15.77 kD   |   pI: 8.74   |   ext: 21430   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T4 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical, Biomedical: Transcription Factor, PSI-Biology
projects EPI list B     HCPIN     Transcription Factor     PSI:Biology Partner - EpiG    
best PDB match calculating ...
informatics Prof PHD data   |   Pxs: 0.157   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures1.10.150.60 details   46774 details   PF01388 details   DOMAIN:ARID details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4394C).
Display constructs associated with this target (HR4394C) and all other associated targets (HR4394, HR4394A, HR4394B).


Domain and UniProt Features
    SuperFamily 46774
    CATH 1.10.150.60
    PFAM PF01388
    UniProt DOMAIN:ARID
    ARID. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:REKLES
    REKLES. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR4394A
    HR4394C
    HR4394B

HR4394

       PDB seq homology



Constructs and Most Advanced Status
   
HR4394C-SGCAv.*

   
HR4394C-15.*

   
HR4394C-SGCAV.*

   
HR4394C-AvMBPB2.*

   
HR4394C-218-351-TEV.*

   
HR4394C-Av6HT.*

   
HR4394C-14.*

   
HR4394C-218-351-Av6HT.*

   
HR4394C-AvSUMO.*



most recent record
2 years, 17 days ago --     purification [14.4 mg]: HR4394C-14.6-LBa-Ni

2007-05-23    target: HR4394C
2007-12-14    construct: HR4394C-15.1
2008-01-24    expression [ 3, 5 ] : HR4394C-15.1-ss
2008-01-29    expression [ 3, 4 ] : HR4394C-15.1-NC5a
2008-02-07    purification [37.3 mg]: HR4394C-15.1-NC5a-Ni
2008-02-12     HR4394C.001 @ Rutgers; 0.012 ml at 0.67 mM
2008-02-25    HSQC [good]: HR4394C.001.600MHz.20C
2008-02-12     HR4394C.002 @ Rutgers; 0.2 ml at 0.67 mM
2008-02-12     HR4394C.003 @ Rutgers; 1.7 ml at 1.071 mM
2008-01-29    expression [ 3, 4 ] : HR4394C-15.1-NC5b
2008-02-07    purification [37.3 mg]: HR4394C-15.1-NC5b-Ni
2008-02-27    expression [ 3, 3 ] : HR4394C-15.1-NC5c
2008-03-13    purification [45.7 mg]: HR4394C-15.1-NC5c-GF
2008-02-27    expression [ 3, 3 ] : HR4394C-15.1-NC5d
2008-03-13    purification [45.7 mg]: HR4394C-15.1-NC5d-GF
2009-01-07     aggregation screening [monomer,15.47 kD,95.8%]
2008-03-25     hr4394c.006 @ Rutgers; 0.3 ml at 1.195 mM
2008-07-17    HSQC [promising]: HR4394C.006.500MHz.20C
2009-11-15     HR4394C.007 @ Rutgers; 0.227 ml at 2.474 mM
2009-04-30     HR4394C.018 @ UGA; 0.5 ml at 1.2 mM
2011-10-28     HR4394C.019 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-11-22     HR4394C.020 @ RAN - Toronto; 1 ml at 1 mg/mL
2012-01-30     HR4394C.021 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2012-03-14     Entered Ab Pipeline: 2012-03-14; 44 days after shipment
2012-10-24     Exited Ab Pipeline: 2012-10-24; 224 days after entering
2012-01-30     Passing antibodies: 1
2008-03-04    expression [ 2, 5 ] : HR4394C-15.1-NCa
2008-03-13    purification [32.0 mg]: HR4394C-15.1-NCa-GF
2008-03-25     hr4394c.004 @ Rutgers; 0.3 ml at 1.103 mM
2008-07-17    HSQC [promising]: HR4394C.004.500MHz.20C
2009-11-15     HR4394C.005 @ Rutgers; 1.3 ml at 1.103 mM
2009-01-16     HR4394C.016 @ Rutgers; 0.03 ml at 0.886 mM
2008-03-04    expression [ 2, 5 ] : HR4394C-15.1-NCb
2008-03-13    purification [32.0 mg]: HR4394C-15.1-NCb-GF
2008-03-04    expression [ 2, 5 ] : HR4394C-15.1-NC5e
2008-03-13    purification [45.7 mg]: HR4394C-15.1-NC5e-GF
2008-03-04    expression [ 2, 5 ] : HR4394C-15.1-NC5f
2008-03-13    purification [45.7 mg]: HR4394C-15.1-NC5f-GF
2008-07-02    expression [ 3, 3 ] : HR4394C-15.1-LBa
2008-03-13    construct: HR4394C-14.2
2008-08-28    construct: HR4394C-14.6
2008-09-26    expression [ 3, 5 ] : HR4394C-14.6-ss
2008-10-21    expression [ 3, 4 ] : HR4394C-14.6-NC5b
2008-10-28    purification [13.0 mg]: HR4394C-14.6-NC5b-Ni
2008-10-29    purification [11.9 mg]: HR4394C-14.6-NC5b-GF
2008-10-21    expression [ 3, 3 ] : HR4394C-14.6-NC5a
2008-10-28    purification [13.0 mg]: HR4394C-14.6-NC5a-Ni
2008-10-29    purification [11.9 mg]: HR4394C-14.6-NC5a-GF
2008-10-31     HR4394C.010 @ Rutgers; 0.5 ml at 0.95 mM
2008-12-10    expression [ 4, 4 ] : HR4394C-14.6-NCb
2008-12-15    purification [75.2 mg]: HR4394C-14.6-NCb-GF
2008-12-18     hr4394c.012 @ Rutgers; 0.3 ml at 0.92 mM
2008-12-18     hr4394c.013 @ Rutgers; 3 ml at 0.92 mM
2009-03-23     HR4394C.017 @ Rutgers; 0.3 ml at 0.92 mM
2009-01-05     HR4394C.014 @ Rutgers; 0.2 ml at 0.92 mM
2009-06-14     NMR structure: 2kk0 (PDB)
2008-12-10    expression [ 4, 4 ] : HR4394C-14.6-NCa
2008-12-15    purification [75.2 mg]: HR4394C-14.6-NCa-GF
2014-11-05    expression [ 5, 5 ] : HR4394C-14.6-LBa
2014-11-10    purification [14.4 mg]: HR4394C-14.6-LBa-Ni
2014-11-18     HR4394C.028 @ Gordon Whiteley - NCI; 0.5 ml at 4.3 mg/mL
2014-11-18     HR4394C.029 @ Rutgers; 1.6 ml at 4.3 mg/mL
2008-08-28    construct: HR4394C-15.2
2008-07-24    expression [ 3, 5 ] : HR4394C-15.2-LBa
2008-07-29    purification [12.2 mg]: HR4394C-15.2-LBa-Ni
2008-08-11     HR4394C.008 @ Rutgers; 1 ml at 1 mg/mL
2008-08-11     HR4394C.009 @ Rutgers; 3.5 ml at 4.3 mg/mL
2008-09-26    expression [ 3, 5 ] : HR4394C-15.2-ss
2008-11-11    expression [ 3, 4 ] : HR4394C-15.2-NC5b
2008-11-25    purification [28.4 mg]: HR4394C-15.2-NC5b-GF
2008-12-12     HR4394C.011 @ Rutgers; 0.012 ml at 0.968 mM
2009-02-27    HSQC [good]: HR4394C.011.600MHz.20C
2009-01-08     HR4394C.015 @ Rutgers; 0.5 ml at 0.968 mM
2008-11-11    expression [ 3, 4 ] : HR4394C-15.2-NC5a
2008-11-25    purification [28.4 mg]: HR4394C-15.2-NC5a-GF
2011-01-18    construct: HR4394C-218-351-TEV.7
2011-02-01    expression [ 4, 4, not tested ]: HR4394C-218-351-TEV.7-ss
2013-09-18    expression [ 3, 3, not tested ]: HR4394C-218-351-TEV.7-SeMa
2011-01-18    construct: HR4394C-218-351-TEV.8
2011-02-01    expression [ 4, 4, not tested ]: HR4394C-218-351-TEV.8-ss
2012-09-28    construct: HR4394C-218-351-Av6HT.10
2012-10-15    expression [ 3, 1, not tested ]: HR4394C-218-351-Av6HT.10-ss
2012-09-28    construct: HR4394C-218-351-Av6HT.9
2012-10-15    expression [ 4, 1, not tested ]: HR4394C-218-351-Av6HT.9-ss
2012-11-19    construct: HR4394C-AvMBPB2.11
2013-01-30     HR4394C.022 @ Rutgers; construct sample
2012-12-11    expression [ 4, 5, not tested ]: HR4394C-AvMBPB2.11-ss
2012-11-19    construct: HR4394C-AvMBPB2.12
2013-01-30     HR4394C.023 @ Rutgers; construct sample
2012-12-11    expression [ 5, 5, not tested ]: HR4394C-AvMBPB2.12-ss
2013-02-05    expression [ 5, 5, not tested ]: HR4394C-AVMBPB2.12-LBa
2013-02-21    purification [76.8 mg]: HR4394C-AVMBPB2.12-LBa-GF
2013-04-17     aggregation screening [monomer,65.12 kD,91.4%]
2013-02-25     HR4394C.024 @ Rutgers; 14.9 ml at 5.3 mg/mL
2013-02-25     HR4394C.025 @ RAN - Chicago; 1.5 ml at 1.19 mg/mL
2013-04-16     HR4394C.026 @ HWI; 0.1 ml at 5.3 mg/mL
2012-11-19    construct: HR4394C-SGCAv.13
2012-12-11    expression [ 0, 0 ] : HR4394C-SGCAv.13-ss
2012-11-19    construct: HR4394C-SGCAv.14
2012-12-11    expression [ 0, 0 ] : HR4394C-SGCAv.14-ss
2012-11-19    construct: HR4394C-Av6HT.15
2013-10-03     HR4394C.027 @ Rutgers; construct sample
2012-12-11    expression [ 4, 5, not tested ]: HR4394C-Av6HT.15-ss
2012-11-19    construct: HR4394C-Av6HT.16
2012-12-11    expression [ 4, 3, not tested ]: HR4394C-Av6HT.16-ss
2013-09-10    construct: HR4394C-AvSUMO.19
2013-09-23    expression [ 2, 4, not tested ]: HR4394C-AvSUMO.19-ss
2013-09-10    construct: HR4394C-AvSUMO.20
2013-09-23    expression [ 2, 4, not tested ]: HR4394C-AvSUMO.20-ss
2014-01-09    construct: HR4394C-AvMBPB2.29
2014-01-31    expression [ 3, 5, not tested ]: HR4394C-AvMBPB2.29-ss
2014-01-09    construct: HR4394C-AvMBPB2.30
2014-01-31    expression [ 3, 5, not tested ]: HR4394C-AvMBPB2.30-ss
2014-01-09    construct: HR4394C-SGCAV.33
2014-01-31    expression [ -1, -1 ] : HR4394C-SGCAV.33-ss
2014-01-09    construct: HR4394C-SGCAV.34
2014-01-31    expression [ -1, -1 ] : HR4394C-SGCAV.34-ss


Available samples:

LB samples
HR4394C.025 @ RAN - Chicago HR4394C.024 @ Rutgers HR4394C.009 @ Rutgers HR4394C.008 @ Rutgers HR4394C.028 @ Gordon Whiteley - NCI
HR4394C.026 @ HWI HR4394C.029 @ Rutgers


NC samples
HR4394C.016 @ Rutgers hr4394c.012 @ Rutgers HR4394C.014 @ Rutgers
HR4394C.005 @ Rutgers HR4394C.017 @ Rutgers hr4394c.013 @ Rutgers hr4394c.004 @ Rutgers


NC5 samples
HR4394C.018 @ UGA
HR4394C.007 @ Rutgers HR4394C.011 @ Rutgers hr4394c.006 @ Rutgers HR4394C.002 @ Rutgers HR4394C.001 @ Rutgers
HR4394C.003 @ Rutgers HR4394C.021 @ CDI Mayaguez, PR HR4394C.019 @ JHU/CDI Baltimore, MD HR4394C.020 @ RAN - Toronto HR4394C.015 @ Rutgers
HR4394C.010 @ Rutgers


E-mail communications associated with this target:

  2013-01-31
  2013-02-19
  2013-04-18
  2013-04-18
  2013-04-18
  2013-04-18
  2013-04-24
  2013-04-24
  2013-05-02
  2013-05-02
  2013-10-10
  2013-11-20