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HR4435B   (SATB1)

5 targets assoicated with this target.
HR4435parentselected
HR4435Asibling(1-352)selected
HR4435Csibling(368-452)soluble construct with excellent ES score
HR4435Dsibling(53-254)purified
HR4435Esibling(53-178)HSQC collected

database refs UniProt: Q01826 (SATB1_HUMAN)   |   HUGO: SATB1   |   NCBI gene: 6304   |   refSeq: NP_001124482.1; NP_001182399.1; NP_002962.1
status NMR and X-Ray structures
structures 2L1P (NMR)   3NZL (Xray)  
properties length: 53 aa   |   mass: 6.09 kD   |   pI: 9.30   |   ext: 9970   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures47413 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4435B).
Display constructs associated with this target (HR4435B) and all other associated targets (HR4435, HR4435E, HR4435D, HR4435C, HR4435A).


Domain and UniProt Features
    Nuclear localization signal domain_number-1
    UniProt MOTIF:Nuclear localization signal
    UniProt MOTIF:Protein interaction
    Protein interaction domain_number-1
    Cleavage; by caspases domain_number-1
    UniProt SITE:Cleavage; by caspases
    SuperFamily 47413
    CUT 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:CUT 1
    PFAM PF02376
    CATH 1.10.260.40
    Binding - Matrix attachment region (MAR) DNA domain_number-1
    UniProt BINDING:Matrix attachment region (MAR) DNA
    Binding - Matrix attachment region (MAR) DNA domain_number-2
    SuperFamily 47413
    CUT 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:CUT 2
    PFAM PF02376
    CATH 1.10.260.40
    Homeobox. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:Homeobox


NESG targets
    HR4435A
    HR4435D
    HR4435E
    HR4435B
    HR4435C

HR4435

       PDB seq homology



Constructs and Most Advanced Status
   
HR4435D-53-254-15.*

   
HR4435E-53-178-15.*

   
HR4435E-56-175-15.*

   
HR4435D-56-250-15.*

   
HR4435B-174-250-14.*

   
HR4435B-174-254-14.*

   
HR4435B-179-244-14.*

   
HR4435B-179-250-14.*

   
HR4435C-368-452-TEV.*



most recent record
7 years, 24 days ago --     NMR structure: 2l1p

2009-11-12    target: HR4435B
PLIMS data     Construct creation records
PLIMS data     Construct transfection records
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE analytical scores
2010-01-11    construct: HR4435B-179-250-14.10
2010-02-05    expression [ 5, 5 ] : HR4435B-179-250-14.10-ss
2010-02-23    expression [ 5, 5 ] : HR4435B-179-250-14.10-NC5a
2010-03-03    purification [24.8 mg]: HR4435B-179-250-14.10-NC5a-GF
2010-03-23     aggregation screening [monomer,11.8 kD,98.6%]
2010-03-12     HR4435B.002 @ Rutgers; 2.5 ml at 1.06 mM
2010-03-12     HR4435B.001 @ Rutgers; 0.04 ml at 1.06 mM
2010-06-07    HSQC [good]: HR4435B.001.600MHz.20C
2010-03-17    expression [ 5, 5 ] : HR4435B-179-250-14.10-SeMa
2010-03-24    purification [33.6 mg]: HR4435B-179-250-14.10-SeMa-GF
2010-03-30     aggregation screening [monomer,10.2 kD,81.1%|monomer,11.5 kD,17.0%]
2010-03-31     HR4435B.006 @ Columbia; 2 ml at 7.9 mg/mL
2010-07-16     X-Ray structure: 3nzl (PDB)
2010-03-31     HR4435B.007 @ HWI; 0.45 ml at 7.9 mg/mL
2010-03-31     HR4435B.008 @ Powers; 4 ml at 2 mg/mL
2010-03-31    expression [ 5, 5 ] : HR4435B-179-250-14.10-NCa
2010-04-05    purification [34.4 mg]: HR4435B-179-250-14.10-NCa-GF
2010-04-07     hr4435b.009 @ Rutgers; 0.3 ml at 1.08 mM
2010-06-07    HSQC [good]: HR4435B.009.600MHz.20C
2010-08-02     NMR structure: 2l1p (PDB)
2010-04-07     hr4435b.010 @ Rutgers; 3.2 ml at 1.08 mM
2010-04-06    expression [ 4, 5 ] : HR4435B-179-250-14.10-NC5b
2010-04-12    purification [23.6 mg]: HR4435B-179-250-14.10-NC5b-GF
2010-04-14     hr4435b.014 @ Rutgers; 0.5 ml at 1.07 mM
2010-04-14     HR4435B.015 @ Powers; 7.5 ml at 2 mg/mL
2010-04-21     HR4435B.016 @ UGA; 0.5 ml at 1.07 mM
2010-07-26     HR4435B.017 @ Rutgers; 0.04 ml at 1.07 mM
2010-01-11    construct: HR4435B-179-244-14.12
2010-02-05    expression [ 3, 5 ] : HR4435B-179-244-14.12-ss
2010-01-07    construct: HR4435B-174-254-14.1
2010-02-05    expression [ 4, 4 ] : HR4435B-174-254-14.1-ss
2010-01-07    construct: HR4435B-174-254-14.2
2010-02-05    expression [ 4, 5 ] : HR4435B-174-254-14.2-ss
2010-02-23    expression [ 4, 5 ] : HR4435B-174-254-14.2-NC5a
2010-03-03    purification [15.7 mg]: HR4435B-174-254-14.2-NC5a-GF
2010-03-12     HR4435B.005 @ Rutgers; 1.5 ml at 0.56 mM
2010-02-23    expression [ 3, 5 ] : HR4435B-174-254-14.2-SeMa
2010-01-07    construct: HR4435B-174-250-14.3
2010-02-05    expression [ 5, 5 ] : HR4435B-174-250-14.3-ss
2010-01-07    construct: HR4435B-174-250-14.4
2013-10-03     HR4435B.018 @ Rutgers; construct sample
2010-02-05    expression [ 5, 5 ] : HR4435B-174-250-14.4-ss
2010-02-23    expression [ 5, 5 ] : HR4435B-174-250-14.4-NC5a
2010-03-03    purification [81.2 mg]: HR4435B-174-250-14.4-NC5a-GF
2010-03-23     aggregation screening [monomer,11.27 kD,95.4%]
2010-03-12     HR4435B.003 @ Rutgers; 0.04 ml at 0.7 mM
2010-06-07    HSQC [good]: HR4435B.003.600MHz.20C
2010-03-12     HR4435B.004 @ Rutgers; 6.9 ml at 0.7 mM
2010-03-17    expression [ 5, 5 ] : HR4435B-174-250-14.4-SeMa
2010-03-29    purification [46.0 mg]: HR4435B-174-250-14.4-SeMa-GF
2010-04-13     aggregation screening [monomer,10.96 kD,79.2%|monomer,12.71 kD,10.7%]
2011-08-04     HR4435B.019 @ Columbia; 0.1 ml at 7.23 mg/mL
2010-04-12     HR4435b.011 @ Columbia; 2 ml at 7.23 mg/mL
2010-04-12     HR4435B.012 @ HWI; 0.45 ml at 7.23 mg/mL
2010-04-12     HR4435B.013 @ Rutgers; 2.261 ml at 7.23 mg/mL
2011-10-28     HR4435B.020 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-11-21     HR4435B.021 @ RAN - Toronto; 1 ml at 1 mg/mL
2012-01-27     HR4435B.022 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2012-03-13     Entered Ab Pipeline: 2012-03-13; 46 days after shipment
2014-01-09     Exited Ab Pipeline: 2014-01-09; 667 days after entering
2012-01-27     Passing antibodies: 2
2010-01-07    construct: HR4435B-179-250-14.5
2010-02-05    expression [ 5, 5 ] : HR4435B-179-250-14.5-ss


Available samples:

SeMet samples
HR4435B.013 @ Rutgers HR4435B.008 @ Powers HR4435B.020 @ JHU/CDI Baltimore, MD HR4435B.012 @ HWI HR4435B.007 @ HWI
HR4435B.022 @ CDI Mayaguez, PR HR4435B.006 @ Columbia HR4435b.011 @ Columbia HR4435B.021 @ RAN - Toronto HR4435B.019 @ Columbia


NC samples
hr4435b.009 @ Rutgers hr4435b.010 @ Rutgers


NC5 samples
HR4435B.005 @ Rutgers HR4435B.016 @ UGA HR4435B.001 @ Rutgers
HR4435B.017 @ Rutgers HR4435B.003 @ Rutgers HR4435B.015 @ Powers HR4435B.002 @ Rutgers hr4435b.014 @ Rutgers
HR4435B.004 @ Rutgers


E-mail communications associated with this target:

  2013-10-10
  2013-11-20