JavaScript Menu Courtesy of Milonic.com
[nucleotide sequence]     [protein sequence]     [ncbi BLAST]     [add construct]     [add ligand annotation]

HR4435D   (SATB1)

5 targets assoicated with this target.
HR4435parentselected
HR4435Asibling(1-352)selected
HR4435Csibling(368-452)soluble construct with excellent ES score
HR4435Esibling(53-178)HSQC collected
HR4435Bsibling(193-245)NMR and X-Ray structures

database refs UniProt: Q01826 (SATB1_HUMAN)   |   HUGO: SATB1   |   NCBI gene: 6304   |   refSeq: NP_001124482.1; NP_001182399.1; NP_002962.1
status purified
properties length: 202 aa   |   mass: 22.92 kD   |   pI: 6.53   |   ext: 28420   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesMOTIF:Protein interaction details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4435D).
Display constructs associated with this target (HR4435D) and all other associated targets (HR4435, HR4435E, HR4435B, HR4435C, HR4435A).


Domain and UniProt Features
    Nuclear localization signal domain_number-1
    UniProt MOTIF:Nuclear localization signal
    UniProt MOTIF:Protein interaction
    Protein interaction domain_number-1
    Cleavage; by caspases domain_number-1
    UniProt SITE:Cleavage; by caspases
    SuperFamily 47413
    CUT 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:CUT 1
    PFAM PF02376
    CATH 1.10.260.40
    Binding - Matrix attachment region (MAR) DNA domain_number-1
    UniProt BINDING:Matrix attachment region (MAR) DNA
    Binding - Matrix attachment region (MAR) DNA domain_number-2
    SuperFamily 47413
    CUT 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:CUT 2
    PFAM PF02376
    CATH 1.10.260.40
    Homeobox. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:Homeobox


NESG targets
    HR4435A
    HR4435D
    HR4435E
    HR4435B
    HR4435C

HR4435

       PDB seq homology



Constructs and Most Advanced Status
   
HR4435D-53-254-15.*

   
HR4435E-53-178-15.*

   
HR4435E-56-175-15.*

   
HR4435D-56-250-15.*

   
HR4435B-174-250-14.*

   
HR4435B-174-254-14.*

   
HR4435B-179-244-14.*

   
HR4435B-179-250-14.*

   
HR4435C-368-452-TEV.*



most recent record
5 years, 26 days ago --     purification [24.6 mg]: HR4435D-53-254-15.1-NC5b-Ni; purification [26.9 mg]: HR4435D-53-254-15.1-NC5b-GF; purification [24.6 mg]: HR4435D-53-254-15.1-NC5a-Ni; purification [26.9 mg]: HR4435D-53-254-15.1-NC5a-GF

2011-02-28    target: HR4435D
2011-04-07    construct: HR4435D-53-254-15.1
2011-06-07    expression [ 4, 4 ] : HR4435D-53-254-15.1-ss
2011-08-23    expression [ 5, 2 ] : HR4435D-53-254-15.1-NC5b
2011-10-03    purification [24.6 mg]: HR4435D-53-254-15.1-NC5b-Ni
2011-10-03    purification [26.9 mg]: HR4435D-53-254-15.1-NC5b-GF
2011-08-23    expression [ 5, 3 ] : HR4435D-53-254-15.1-NC5a
2011-10-03    purification [24.6 mg]: HR4435D-53-254-15.1-NC5a-Ni
2011-10-03    purification [26.9 mg]: HR4435D-53-254-15.1-NC5a-GF
2011-10-11     HR4435D.001 @ Rutgers; 0.05 ml at 0.08 mM
2011-04-07    construct: HR4435D-53-254-15.2
2011-06-07    expression [ 3, 5 ] : HR4435D-53-254-15.2-ss
2011-04-07    construct: HR4435D-56-250-15.3
2011-06-07    expression [ 4, 2 ] : HR4435D-56-250-15.3-ss
2011-04-07    construct: HR4435D-56-250-15.4
2011-06-07    expression [ 2, 5 ] : HR4435D-56-250-15.4-ss


Available samples:

NC5 samples
HR4435D.001 @ Rutgers