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HR4435E   (SATB1)

5 targets assoicated with this target.
HR4435parentselected
HR4435Asibling(1-352)selected
HR4435Csibling(368-452)soluble construct with excellent ES score
HR4435Dsibling(53-254)purified
HR4435Bsibling(193-245)NMR and X-Ray structures

database refs UniProt: Q01826 (SATB1_HUMAN)   |   HUGO: SATB1   |   NCBI gene: 6304   |   refSeq: NP_001124482.1; NP_001182399.1; NP_002962.1
status HSQC collected
properties length: 126 aa   |   mass: 14.15 kD   |   pI: 5.94   |   ext: 12950   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesMOTIF:Protein interaction details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4435E).
Display constructs associated with this target (HR4435E) and all other associated targets (HR4435, HR4435D, HR4435B, HR4435C, HR4435A).


Domain and UniProt Features
    Nuclear localization signal domain_number-1
    UniProt MOTIF:Nuclear localization signal
    UniProt MOTIF:Protein interaction
    Protein interaction domain_number-1
    Cleavage; by caspases domain_number-1
    UniProt SITE:Cleavage; by caspases
    SuperFamily 47413
    CUT 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:CUT 1
    PFAM PF02376
    CATH 1.10.260.40
    Binding - Matrix attachment region (MAR) DNA domain_number-1
    UniProt BINDING:Matrix attachment region (MAR) DNA
    Binding - Matrix attachment region (MAR) DNA domain_number-2
    SuperFamily 47413
    CUT 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:CUT 2
    PFAM PF02376
    CATH 1.10.260.40
    Homeobox. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:Homeobox


NESG targets
    HR4435A
    HR4435D
    HR4435E
    HR4435B
    HR4435C

HR4435

       PDB seq homology



Constructs and Most Advanced Status
   
HR4435D-53-254-15.*

   
HR4435E-53-178-15.*

   
HR4435E-56-175-15.*

   
HR4435D-56-250-15.*

   
HR4435B-174-250-14.*

   
HR4435B-174-254-14.*

   
HR4435B-179-244-14.*

   
HR4435B-179-250-14.*

   
HR4435C-368-452-TEV.*



most recent record
4 years, 1 day ago --     expression [5, 1]: HR4435E-56-175-15.4-LBa

2011-02-28    target: HR4435E
2011-04-07    construct: HR4435E-53-178-15.1
2011-06-07    expression [ 5, 5 ] : HR4435E-53-178-15.1-ss
2011-04-07    construct: HR4435E-53-178-15.2
2011-06-07    expression [ 5, 5 ] : HR4435E-53-178-15.2-ss
2011-06-22    expression [ 5, 4 ] : HR4435E-53-178-15.2-SeMa
2011-07-12    purification [44.0 mg]: HR4435E-53-178-15.2-SeMa-GF
2011-07-29     HR4435E.007 @ Rutgers; 20 ml at 2.2 mg/mL
2011-06-22    expression [ 5, 4 ] : HR4435E-53-178-15.2-NC5a
2011-06-28    purification [60.8 mg]: HR4435E-53-178-15.2-NC5a-Ni
2011-06-29    purification [40.0 mg]: HR4435E-53-178-15.2-NC5a-GF
2011-07-02     HR4435E.001 @ Rutgers; 0.04 ml at 0.565 mM
2011-07-22    HSQC [poor]: HR4435E.001.600MHz.20C
2011-07-02     HR4435E.002 @ Rutgers; 4 ml at 0.565 mM
2011-07-02     HR4435E.003 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-07-02     HR4435E.004 @ Gordon Whiteley - NCI; 1 ml at 1 mg/mL
2011-07-02     HR4435E.005 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2012-03-14     Entered Ab Pipeline: 2012-03-14; 256 days after shipment
2013-04-19     Exited Ab Pipeline: 2013-04-19; 401 days after entering
2011-07-02     Passing antibodies: 5
2011-07-02     HR4435E.006 @ Rutgers; 1 ml at 1 mg/mL
2012-01-30     HR4435E.012 @ Rutgers; 1 ml at 1 mg/mL
2011-04-07    construct: HR4435E-56-175-15.3
2011-06-07    expression [ 5, 5 ] : HR4435E-56-175-15.3-ss
2011-04-07    construct: HR4435E-56-175-15.4
2011-06-07    expression [ 5, 5 ] : HR4435E-56-175-15.4-ss
2011-06-22    expression [ 5, 3 ] : HR4435E-56-175-15.4-SeMa
2011-07-14    purification [18.2 mg]: HR4435E-56-175-15.4-SeMa-GF
2011-07-26     aggregation screening [Aggregated,48700 kD,54.2%]
2011-08-03     HR4435E.009 @ Columbia; 0.7 ml at 5.8 mg/mL
2011-08-03     HR4435E.008 @ HWI; 0.45 ml at 5.8 mg/mL
2011-06-22    expression [ 5, 3 ] : HR4435E-56-175-15.4-NC5a
2011-09-14    purification [31.2 mg]: HR4435E-56-175-15.4-NC5a-GF
2011-10-05     HR4435E.010 @ Rutgers; 0.04 ml at 0.87 mM
2011-10-26    HSQC [poor]: HR4435E.010.600MHz.20C
2011-10-05     HR4435E.011 @ Rutgers; 1.96 ml at 0.87 mM
2013-03-28    expression [ 5, 1 ] : HR4435E-56-175-15.4-LBa


Available samples:

SeMet samples
HR4435E.007 @ Rutgers HR4435E.009 @ Columbia HR4435E.008 @ HWI


NC5 samples
HR4435E.001 @ Rutgers HR4435E.011 @ Rutgers
HR4435E.012 @ Rutgers HR4435E.006 @ Rutgers HR4435E.002 @ Rutgers HR4435E.005 @ CDI Mayaguez, PR HR4435E.010 @ Rutgers
HR4435E.003 @ JHU/CDI Baltimore, MD HR4435E.004 @ Gordon Whiteley - NCI