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HR4436B   (BCL6)

3 targets assoicated with this target.
HR4436parentselected
HR4436Asibling(130-456)selected
HR4436Csibling(5-129)purified

database refs UniProt: P41182 (BCL6_HUMAN)   |   HUGO: BCL6   |   NCBI gene: 604   |   refSeq: NP_001124317.1; NP_001128210.1; NP_001697.2
status NMR structure and crystal hits
structures 2LCE (NMR)  
properties length: 63 aa   |   mass: 7.21 kD   |   pI: 9.75   |   ext: 4470   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesPF13465 details   PF00096 details   3.30.160.60 details   ZN_FING:C2H2-type 2 details   ZN_FING:C2H2-type 3 details   57667 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4436B).
Display constructs associated with this target (HR4436B) and all other associated targets (HR4436, HR4436C, HR4436A).


Domain and UniProt Features
    CATH 3.30.710.10
    SuperFamily 54695
    PFAM PF00651
    BTB. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:BTB
    Zinc finger - C2H2-type 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 1
    CATH 3.30.160.60
    Zinc finger - C2H2-type 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 2
    CATH 3.30.160.60
    SuperFamily 57667
    Zinc finger - C2H2-type 3. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 3
    SuperFamily 57667
    CATH 3.30.160.60
    SuperFamily 57667
    PFAM PF13465
    UniProt ZN_FING:C2H2-type 4
    Zinc finger - C2H2-type 4. {ECO:0000255|PROSITE- domain_number-1
    CATH 3.30.160.60
    PFAM PF13465
    SuperFamily 57667
    UniProt ZN_FING:C2H2-type 5
    Zinc finger - C2H2-type 5. {ECO:0000255|PROSITE- domain_number-1
    CATH 3.30.160.60
    SuperFamily 57667
    PFAM PF13465
    Zinc finger - C2H2-type 6. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    UniProt ZN_FING:C2H2-type 6


NESG targets
    HR4436C
    HR4436A
    HR4436B

HR4436

       PDB seq homology



Constructs and Most Advanced Status
   
HR4436C-5-129-TEV.*

   
HR4436B-523-606-14.*

   
HR4436B-523-602-14.*

   
HR4436B-528-601-14.*

   
HR4436B-540-602-14.*

   
HR4436B-542-598-14.*



most recent record
6 years, 27 days ago --     NMR structure: 2lce

2009-08-17    target: HR4436B
2009-09-15    construct: HR4436B-540-602-14.1
2009-11-04    expression [ 3, 5 ] : HR4436B-540-602-14.1-ss
2010-01-05    expression [ 4, 5 ] : HR4436B-540-602-14.1-SeMb
2010-01-05    purification [21.7 mg]: HR4436B-540-602-14.1-SeMb-GF
2010-01-20     aggregation screening []
2011-11-10     HR4436B.003 @ Rutgers; 0.3 ml at 5.5 mg/mL
2011-11-10     HR4436B.001 @ Rutgers; 2 ml at 5.5 mg/mL
2011-11-10     HR4436B.002 @ HWI; 0.45 ml at 5.5 mg/mL
2010-01-05    expression [ 4, 5 ] : HR4436B-540-602-14.1-SeMa
2010-01-05    purification [21.7 mg]: HR4436B-540-602-14.1-SeMa-GF
2010-05-05    expression [ 4, 5 ] : HR4436B-540-602-14.1-NC5a
2010-05-18    purification [5.7 mg]: HR4436B-540-602-14.1-NC5a-GF
2010-05-27     HR4436B.009 @ Rutgers; 0.04 ml at 0.2 mM
2010-05-27     hr4436b.010 @ Rutgers; 0.46 ml at 0.2 mM
2010-05-05    expression [ 4, 5 ] : HR4436B-540-602-14.1-NC5b
2010-06-02    expression [ 4, 5 ] : HR4436B-540-602-14.1-NC5c
2010-06-10    purification [15.8 mg]: HR4436B-540-602-14.1-NC5c-GF
2010-06-29     aggregation screening [monomer,9.76 kD,81.2%]
2010-06-15     HR4436B.011 @ Rutgers; 0.04 ml at 0.33 mM
2010-07-06    HSQC [good]: HR4436B.011.600MHz.20C
2010-06-15     HR4436B.012 @ Rutgers; 1 ml at 0.65 mM
2010-09-13     HR4436B.017 @ UGA; 0.5 ml at 0.65 mM
2010-09-14    HSQC [good]: HR4436B.017.600MHz.20C
2010-07-08    expression [ 4, 5 ] : HR4436B-540-602-14.1-NC5d
2010-07-27    purification [7.7 mg]: HR4436B-540-602-14.1-NC5d-Ni
2010-07-27    purification [3.5 mg]: HR4436B-540-602-14.1-NC5d-GF
2010-07-30     HR4436B.015 @ UGA; 0.5 ml at 0.15 mM
2010-07-20    expression [ 3, 5 ] : HR4436B-540-602-14.1-NCa
2010-07-27    purification [10.2 mg]: HR4436B-540-602-14.1-NCa-Ni
2010-07-27    purification [12.4 mg]: HR4436B-540-602-14.1-NCa-GF
2010-07-30     HR4436B.013 @ UGA; 0.3 ml at 0.6 mM
2010-08-03    HSQC [good]: HR4436B.013.600MHz.20C
2010-07-30     HR4436B.014 @ Rutgers; 0.5 ml at 0.8 mM
2010-09-13     HR4436B.016 @ UGA; 0.3 ml at 0.8 mM
2010-09-14    HSQC [good]: HR4436B.016.600MHz.20C
2011-04-28     NMR structure: 2lce (PDB)
2011-02-08    expression [ 4, 5 ] : HR4436B-540-602-14.1-NCb
2011-03-07    purification [8.6 mg]: HR4436B-540-602-14.1-NCb-Ni
2011-03-07    purification [15.5 mg]: HR4436B-540-602-14.1-NCb-GF
2011-03-11     HR4436B.021 @ UGA; 0.3 ml at 0.32 mM
2011-03-22    HSQC [good]: HR4436B.021.600MHz.20C
2011-03-11     HR4436B.022 @ Rutgers; 0.2 ml at 0.32 mM
2009-09-15    construct: HR4436B-540-602-14.2
2009-11-04    expression [ 3, 5 ] : HR4436B-540-602-14.2-ss
2009-09-15    construct: HR4436B-542-598-14.3
2009-11-04    expression [ 3, 5 ] : HR4436B-542-598-14.3-ss
2009-09-15    construct: HR4436B-542-598-14.4
2009-11-04    expression [ 3, 5 ] : HR4436B-542-598-14.4-ss
2010-11-10    expression [ 3, 5 ] : HR4436B-542-598-14.4-SeMa
2010-11-16    purification [8.0 mg]: HR4436B-542-598-14.4-SeMa-GF
2010-11-23     aggregation screening [monomer,7.131 kD,85.7%]
2010-12-03     HR4436B.019 @ Columbia; 2 ml at 7.49 mg/mL
2010-12-03     HR4436B.020 @ HWI; 0.45 ml at 7.49 mg/mL
2010-11-10    expression [ 3, 5 ] : HR4436B-542-598-14.4-SeMb
2010-11-16    purification [8.0 mg]: HR4436B-542-598-14.4-SeMb-GF
2009-10-08    construct: HR4436B-542-598-14.7
2010-01-07    construct: HR4436B-523-606-14.5
2010-02-05    expression [ 3, 4 ] : HR4436B-523-606-14.5-ss
2010-01-07    construct: HR4436B-523-606-14.6
2010-02-05    expression [ 3, 5 ] : HR4436B-523-606-14.6-ss
2010-10-26    expression [ 4, 5 ] : HR4436B-523-606-14.6-SeMb
2010-11-02    purification [0.0 mg]: HR4436B-523-606-14.6-SeMb-GF
2010-10-26    expression [ 4, 5 ] : HR4436B-523-606-14.6-SeMa
2010-11-02    purification [0.0 mg]: HR4436B-523-606-14.6-SeMa-GF
2010-01-07    construct: HR4436B-523-602-14.7
2010-02-05    expression [ 3, 4 ] : HR4436B-523-602-14.7-ss
2010-01-07    construct: HR4436B-523-602-14.8
2010-02-05    expression [ 3, 5 ] : HR4436B-523-602-14.8-ss
2010-08-24    expression [ 5, 3 ] : HR4436B-523-602-14.8-SeMa
2010-09-21    purification [0.0 mg]: HR4436B-523-602-14.8-SeMa-GF
2010-10-12     HR4436B.018 @ Rutgers; 20 ml at 3.16 mg/mL
2010-08-24    expression [ 5, 3 ] : HR4436B-523-602-14.8-SeMb
2010-09-21    purification [0.0 mg]: HR4436B-523-602-14.8-SeMb-GF
2010-01-07    construct: HR4436B-528-601-14.9
2010-02-05    expression [ 4, 5 ] : HR4436B-528-601-14.9-ss
2010-01-07    construct: HR4436B-528-601-14.10
2010-02-05    expression [ 5, 5 ] : HR4436B-528-601-14.10-ss
2010-02-23    expression [ 4, 5 ] : HR4436B-528-601-14.10-NC5a
2010-03-04    purification [13.2 mg]: HR4436B-528-601-14.10-NC5a-GF
2010-03-19     HR4436B.004 @ Rutgers; 0.04 ml at 0.396 mM
2010-06-07    HSQC [promising]: HR4436B.004.600MHz.20C
2010-03-15     HR4436B.005 @ Rutgers; 1 ml at 0.4 mM
2010-02-23    expression [ 5, 5 ] : HR4436B-528-601-14.10-SeMa
2010-04-14    purification [45.0 mg]: HR4436B-528-601-14.10-SeMa-GF
2010-04-20     aggregation screening []
2010-11-10     hr4436b.006 @ Rutgers; 2 ml at 4.5 mg/mL
2010-11-10     HR4436B.007 @ HWI; 0.45 ml at 4.5 mg/mL
2010-11-10     HR4436B.008 @ Rutgers; 2.778 ml at 4.5 mg/mL
2011-10-28     HR4436B.023 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-11-21     HR4436B.024 @ RAN - Toronto; 1 ml at 1 mg/mL
2012-01-27     HR4436B.025 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2010-04-13    purification [120.0 mg]: HR4436B-528-601-14.10-SeMa-Ni
2013-10-21     HR4436B.026 @ RAN - Toronto; 0.015 ml at 120 mg/mL


Available samples:

SeMet samples
HR4436B.019 @ Columbia HR4436B.025 @ CDI Mayaguez, PR HR4436B.003 @ Rutgers HR4436B.020 @ HWI HR4436B.024 @ RAN - Toronto
HR4436B.002 @ HWI HR4436B.007 @ HWI hr4436b.006 @ Rutgers HR4436B.018 @ Rutgers HR4436B.001 @ Rutgers
HR4436B.023 @ JHU/CDI Baltimore, MD HR4436B.008 @ Rutgers


NC samples
HR4436B.016 @ UGA HR4436B.013 @ UGA HR4436B.022 @ Rutgers
HR4436B.014 @ Rutgers HR4436B.021 @ UGA


NC5 samples
HR4436B.011 @ Rutgers HR4436B.009 @ Rutgers HR4436B.012 @ Rutgers
HR4436B.005 @ Rutgers HR4436B.017 @ UGA HR4436B.004 @ Rutgers hr4436b.010 @ Rutgers HR4436B.015 @ UGA


E-mail communications associated with this target:

  2013-10-23
  2013-11-15