JavaScript Menu Courtesy of Milonic.com
[nucleotide sequence]     [protein sequence]     [ncbi BLAST]     [add construct]     [add ligand annotation]

HR4490C   (ALX4)

3 targets assoicated with this target.
HR4490parentselected
HR4490Bsibling(332-411)selected
HR4490Asibling(1-90)selected

database refs UniProt: Q9H161 (ALX4_HUMAN)   |   HUGO: ALX4   |   NCBI gene: 60529   |   refSeq: NP_068745.2
status NMR structure
structures 2M0C (NMR)  
properties length: 72 aa   |   mass: 8.98 kD   |   pI: 10.73   |   ext: 15470   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesDNA_BIND:Homeobox details   1.10.10.60 details   46689 details   PF00046 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4490C).
Display constructs associated with this target (HR4490C) and all other associated targets (HR4490, HR4490B, HR4490A).


Domain and UniProt Features
    CATH 1.10.10.60
    SuperFamily 46689
    Homeobox. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:Homeobox
    PFAM PF00046
    OAR. {ECO:0000255|PROSITE- domain_number-1
    UniProt MOTIF:OAR


NESG targets
    HR4490A
    HR4490C
    HR4490B

HR4490

       PDB seq homology



Constructs and Most Advanced Status
   
HR4490C-209-280-NHT.*



most recent record
3 years, 21 days ago --     purification [15.1 mg]: HR4490C-209-280-NHT.2-[LBc,N14a]-Ni

2011-06-14    target: HR4490C
2011-07-13    construct: HR4490C-209-280-NHT.1
2011-07-22    expression [ 5, 4, not tested ]: HR4490C-209-280-NHT.1-ss
2011-07-13    construct: HR4490C-209-280-NHT.2
2011-07-22    expression [ 5, 4, not tested ]: HR4490C-209-280-NHT.2-ss
2011-07-27    expression [ 4, 4, not tested ]: HR4490C-209-280-NHT.2-NC5b
2012-05-24    purification [6.0 mg]: HR4490C-209-280-NHT.2-NC5b-Ni
2012-05-24    purification [1.8 mg]: HR4490C-209-280-NHT.2-NC5b-GF
2012-05-24    purification [6.0 mg]: HR4490C-209-280-NHT.2-NC5b-TV
2011-07-27    expression [ 4, 4, not tested ]: HR4490C-209-280-NHT.2-NC5a
2012-05-24    purification [6.0 mg]: HR4490C-209-280-NHT.2-NC5a-Ni
2012-05-24    purification [1.8 mg]: HR4490C-209-280-NHT.2-NC5a-GF
2012-05-31     HR4490C.001 @ Rutgers; 0.04 ml at 0.56 mM
2012-06-06    HSQC [good]: HR4490C.001.600MHz.20C
2012-05-25     HR4490C.002 @ Buffalo; 0.45 ml at 0.56 mM
2012-05-24    purification [6.0 mg]: HR4490C-209-280-NHT.2-NC5a-TV
2011-07-26    expression [ 4, 4, not tested ]: HR4490C-209-280-NHT.2-SeMa
2011-08-03    purification [4.8 mg]: HR4490C-209-280-NHT.2-SeMa-GF
2012-07-19    expression [ 0, 0, not tested ]: HR4490C-209-280-NHT.2-NC5c
2012-07-19    expression [ 0, 0, not tested ]: HR4490C-209-280-NHT.2-NC5d
2012-07-19    expression [ 3, 4, not tested ]: HR4490C-209-280-NHT.2-NCa
2012-07-26    purification [12.2 mg]: HR4490C-209-280-NHT.2-NCa-Ni
2012-07-26    purification [4.2 mg]: HR4490C-209-280-NHT.2-NCa-TV
2012-08-09    purification [1.8 mg]: HR4490C-209-280-NHT.2-NCa-GF
2012-09-27     aggregation screening [monomer,5.64 kD,98.5%]
2012-09-05     HR4490C.003 @ Buffalo; 0.3 ml at 0.871 mM
2012-10-24     NMR structure: 2m0c (PDB)
2012-07-19    expression [ 3, 4, not tested ]: HR4490C-209-280-NHT.2-NCb
2012-07-26    purification [12.2 mg]: HR4490C-209-280-NHT.2-NCb-Ni
2012-07-26    purification [4.2 mg]: HR4490C-209-280-NHT.2-NCb-TV
2012-08-09    purification [1.8 mg]: HR4490C-209-280-NHT.2-NCb-GF
2012-08-01    expression [ 4, 5, not tested ]: HR4490C-209-280-NHT.2-NC5e
2013-03-19    purification [9.6 mg]: HR4490C-209-280-NHT.2-NC5e-Ni
2012-08-01    expression [ 4, 5, not tested ]: HR4490C-209-280-NHT.2-NC5f
2013-03-28    expression [ 2, 0, not tested ]: HR4490C-209-280-NHT.2-LBa
2013-04-04    purification [0.0 mg]: HR4490C-209-280-NHT.2-LBa-Ni
2013-04-04    expression [ 5, 2, not tested ]: HR4490C-209-280-NHT.2-LBb
2013-11-05    purification [18.0 mg]: HR4490C-209-280-NHT.2-LBb-Ni
2013-11-11     HR4490C.004 @ Rutgers; 1 ml at 0.07 mg/mL
2013-11-11     HR4490C.005 @ Rutgers; 0.7 ml at 0.157 mg/mL
2013-11-18    expression [ 5, 0, not tested ]: HR4490C-209-280-NHT.2-LBc
2014-01-06    purification [15.1 mg]: HR4490C-209-280-NHT.2-[LBc,N14a]-Ni
2014-01-22     HR4490C.006 @ CDI Mayaguez, PR; 2 ml at 0.343 mg/mL
2014-01-22     HR4490C.007 @ JHU/CDI Baltimore, MD; 0.5 ml at 0.234 mg/mL
2014-01-27     HR4490C.008 @ Rutgers; 2 ml at 0.343 mg/mL
2013-11-18    expression [ 4, 1, not tested ]: HR4490C-209-280-NHT.2-N14a


Available samples:

LB samples
HR4490C.005 @ Rutgers HR4490C.004 @ Rutgers


Labeling not defined samples
HR4490C.008 @ Rutgers HR4490C.006 @ CDI Mayaguez, PR HR4490C.007 @ JHU/CDI Baltimore, MD


NC samples
HR4490C.003 @ Buffalo


NC5 samples
HR4490C.002 @ Buffalo HR4490C.001 @ Rutgers


E-mail communications associated with this target:

  2012-09-04
  2012-09-05
  2014-02-06
  2014-02-07
  2014-02-10
  2014-02-18