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HR4527D   (ILF3)

5 targets assoicated with this target.
HR4527parentselected
HR4527Asibling(116-374)selected
HR4527Bsibling(399-482)selected
HR4527Csibling(111-342)expression tested
HR4527Esibling(520-594)NMR and X-Ray structures

database refs UniProt: Q12906 (ILF3_HUMAN)   |   HUGO: ILF3   |   NCBI gene: 3609   |   refSeq: NP_001131145.1; NP_004507.2; NP_036350.2; NP_060090.2; NP_703194.1
status good HSQC collected    work stopped (2010-06-30; End of PSI-2)
properties length: 90 aa   |   mass: 9.82 kD   |   pI: 9.40   |   ext: 2980   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures54768 details   DOMAIN:DRBM 2 details   3.30.160.20 details   PF00035 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4527D).
Display constructs associated with this target (HR4527D) and all other associated targets (HR4527, HR4527E, HR4527C, HR4527A, HR4527B).


Domain and UniProt Features
    DZF. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF07528
    UniProt DOMAIN:DZF
    Bipartite nuclear localization signal domain_number-1
    UniProt MOTIF:Bipartite nuclear localization signal (Potential)
    CATH 3.30.160.20
    UniProt DOMAIN:DRBM 1
    DRBM 1. {ECO:0000255|PROSITE- domain_number-1
    CATH 3.30.160.20
    DRBM 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:DRBM 2
    PFAM PF00035


NESG targets
    HR4527C
    HR4527A
    HR4527B
    HR4527D
    HR4527E

HR4527

       PDB seq homology



Constructs and Most Advanced Status
   
HR4527C-14.*

   
HR4527D-508-600-14.*

   
HR4527D-508-572-14.*

   
HR4527D-513-593-14.*

   
HR4527E-515-572-14.*

   
HR4527E-515-600-14.*

   
HR4527E-520-593-14.*

   
HR4527E-520-572-14.*

   
HR4527E-521-600-14.*

   
HR4527E-526-594-14.*

   
HR4527E-537-594-14.*



most recent record
7 years, 21 days ago --     HSQC [good]; HSQC [good]

2009-11-12    target: HR4527D     work stopped (2010-06-30; End of PSI-2)
2010-01-29    construct: HR4527D-508-600-14.1     work stopped (2010-06-30; End of PSI-2)
2010-03-08    expression [ 5, 5 ] : HR4527D-508-600-14.1-ss
2010-01-29    construct: HR4527D-508-600-14.2     work stopped (2010-06-30; End of PSI-2)
2010-03-08    expression [ 5, 5 ] : HR4527D-508-600-14.2-ss
2010-03-16    expression [ 4, 5 ] : HR4527D-508-600-14.2-SeMa
2010-04-27    purification [26.0 mg]: HR4527D-508-600-14.2-SeMa-GF
2010-05-04     aggregation screening [monomer,12.86 kD,87.1%]
2010-05-04     HR4527D.001 @ Columbia; 2 ml at 5.3 mg/mL
2010-05-04     HR4527D.002 @ HWI; 0.45 ml at 5.3 mg/mL
2010-05-04     HR4527D.003 @ Rutgers; 0.5 ml at 5.3 mg/mL
2010-05-05    expression [ 4, 5 ] : HR4527D-508-600-14.2-NC5a
2010-05-18    purification [19.5 mg]: HR4527D-508-600-14.2-NC5a-GF
2010-06-29     aggregation screening [monomer,12.32 kD,98.9%]
2010-05-27     HR4527D.007 @ Rutgers; 0.04 ml at 0.7 mM
2010-07-06    HSQC [good]: HR4527D.007.600MHz.20C
2010-05-27     hr4527d.008 @ Rutgers; 0.96 ml at 0.7 mM
2010-01-29    construct: HR4527D-513-593-14.3     work stopped (2010-06-30; End of PSI-2)
2010-03-08    expression [ 5, 5 ] : HR4527D-513-593-14.3-ss
2010-01-29    construct: HR4527D-513-593-14.4     work stopped (2010-06-30; End of PSI-2)
2010-03-08    expression [ 5, 5 ] : HR4527D-513-593-14.4-ss
2010-03-23    expression [ 5, 5 ] : HR4527D-513-593-14.4-SeMa
2010-05-05    purification [32.4 mg]: HR4527D-513-593-14.4-SeMa-GF
2010-05-11     aggregation screening [monomer,10.69 kD,56.0%|monomer,11.24 kD,37.2%]
2010-05-07     HR4527D.004 @ Columbia; 2 ml at 9.24 mg/mL
2010-05-07     HR4527D.005 @ HWI; 0.45 ml at 9.24 mg/mL
2010-05-07     HR4527D.006 @ Rutgers; 1 ml at 9.24 mg/mL
2010-05-05    expression [ 4, 5 ] : HR4527D-513-593-14.4-NC5a
2010-05-18    purification [37.3 mg]: HR4527D-513-593-14.4-NC5a-GF
2010-06-29     aggregation screening [monomer,12.4 kD,96.8%]
2010-05-27     HR4527D.009 @ Rutgers; 0.04 ml at 1.5 mM
2010-07-06    HSQC [good]: HR4527D.009.600MHz.20C
2010-05-27     hr4527d.010 @ Rutgers; 0.96 ml at 1.5 mM
2010-03-15    construct: HR4527D-508-572-14.5     work stopped (2010-06-30; End of PSI-2)
2010-04-14    expression [ 0, 0 ] : HR4527D-508-572-14.5-ss
2010-03-15    construct: HR4527D-508-572-14.6     work stopped (2010-06-30; End of PSI-2)
2010-04-14    expression [ 0, 0 ] : HR4527D-508-572-14.6-ss


Available samples:

SeMet samples
HR4527D.003 @ Rutgers HR4527D.004 @ Columbia HR4527D.006 @ Rutgers HR4527D.005 @ HWI HR4527D.002 @ HWI
HR4527D.001 @ Columbia


NC5 samples
hr4527d.010 @ Rutgers hr4527d.008 @ Rutgers HR4527D.009 @ Rutgers HR4527D.007 @ Rutgers