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HR4527E   (ILF3)

5 targets assoicated with this target.
HR4527parentselected
HR4527Asibling(116-374)selected
HR4527Bsibling(399-482)selected
HR4527Csibling(111-342)expression tested
HR4527Dsibling(513-602)good HSQC collected

database refs UniProt: Q12906 (ILF3_HUMAN)   |   HUGO: ILF3   |   NCBI gene: 3609   |   refSeq: NP_001131145.1; NP_004507.2; NP_036350.2; NP_060090.2; NP_703194.1
status NMR and X-Ray structures
structures 2L33 (NMR)   3P1X (Xray)  
properties length: 75 aa   |   mass: 8.30 kD   |   pI: 9.30   |   ext: 2980   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures54768 details   DOMAIN:DRBM 2 details   3.30.160.20 details   PF00035 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4527E).
Display constructs associated with this target (HR4527E) and all other associated targets (HR4527, HR4527D, HR4527C, HR4527A, HR4527B).


Domain and UniProt Features
    DZF. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF07528
    UniProt DOMAIN:DZF
    Bipartite nuclear localization signal domain_number-1
    UniProt MOTIF:Bipartite nuclear localization signal (Potential)
    CATH 3.30.160.20
    UniProt DOMAIN:DRBM 1
    DRBM 1. {ECO:0000255|PROSITE- domain_number-1
    CATH 3.30.160.20
    DRBM 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:DRBM 2
    PFAM PF00035


NESG targets
    HR4527C
    HR4527A
    HR4527B
    HR4527D
    HR4527E

HR4527

       PDB seq homology



Constructs and Most Advanced Status
   
HR4527C-14.*

   
HR4527D-508-600-14.*

   
HR4527D-508-572-14.*

   
HR4527D-513-593-14.*

   
HR4527E-515-572-14.*

   
HR4527E-515-600-14.*

   
HR4527E-520-593-14.*

   
HR4527E-520-572-14.*

   
HR4527E-521-600-14.*

   
HR4527E-526-594-14.*

   
HR4527E-537-594-14.*



most recent record
7 years, 26 days ago --     X-Ray structure: 3p1x

2009-11-12    target: HR4527E
PLIMS data     Construct creation records
PLIMS data     Construct transfection records
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE analytical scores
2010-01-29    construct: HR4527E-515-600-14.1
2010-03-08    expression [ 5, 5 ] : HR4527E-515-600-14.1-ss
2010-01-29    construct: HR4527E-515-600-14.2
2010-03-08    expression [ 5, 5 ] : HR4527E-515-600-14.2-ss
2010-03-16    expression [ 5, 5 ] : HR4527E-515-600-14.2-SeMa
2010-04-27    purification [28.0 mg]: HR4527E-515-600-14.2-SeMa-GF
2010-05-04     aggregation screening [monomer,11.56 kD,81.0%|octamer,92.35 kD,3.8%]
2010-05-04     HR4527E.004 @ Columbia; 2 ml at 7.57 mg/mL
2010-05-04     HR4527E.005 @ HWI; 0.45 ml at 7.57 mg/mL
2010-05-04     HR4527E.006 @ Rutgers; 0.5 ml at 7.57 mg/mL
2010-05-05    expression [ 4, 5 ] : HR4527E-515-600-14.2-NC5a
2010-05-18    purification [0.0 mg]: HR4527E-515-600-14.2-NC5a-GF
2010-01-29    construct: HR4527E-521-600-14.3
2010-03-08    expression [ 5, 5 ] : HR4527E-521-600-14.3-ss
2010-01-29    construct: HR4527E-521-600-14.4
2010-03-08    expression [ 4, 5 ] : HR4527E-521-600-14.4-ss
2010-03-23    expression [ 5, 5 ] : HR4527E-521-600-14.4-SeMa
2010-05-05    purification [36.0 mg]: HR4527E-521-600-14.4-SeMa-GF
2010-05-11     aggregation screening [monomer,11.18 kD,78.9%]
2010-05-07     HR4527E.007 @ Columbia; 2 ml at 7.78 mg/mL
2010-05-07     HR4527E.008 @ HWI; 0.45 ml at 7.78 mg/mL
2010-05-07     HR4527E.009 @ Rutgers; 1 ml at 7.78 mg/mL
2010-05-05    expression [ 4, 5 ] : HR4527E-521-600-14.4-NC5a
2010-05-25    purification [254.8 mg]: HR4527E-521-600-14.4-NC5a-Ni
2010-05-25    purification [98.9 mg]: HR4527E-521-600-14.4-NC5a-GF
2010-06-29     aggregation screening [monomer,13.17 kD,95.2%]
2010-06-17     HR4527E.012 @ Rutgers; 0.04 ml at 1.059 mM
2010-07-06    HSQC [good]: HR4527E.012.600MHz.20C
2010-06-29     HR4527E.013 @ Rutgers; 3 ml at 1.059 mM
2010-07-08    expression [ 4, 5 ] : HR4527E-521-600-14.4-NCa
2010-07-14    purification [141.8 mg]: HR4527E-521-600-14.4-NCa-Ni
2010-07-14    purification [43.8 mg]: HR4527E-521-600-14.4-NCa-GF
2010-07-21     HR4527E.018 @ Rutgers; 0.3 ml at 0.7 mM
2010-07-21     hr4527e.019 @ Rutgers; 2.7 ml at 0.7 mM
2010-09-03     NMR structure: 2l33 (PDB)
2010-07-08    expression [ 4, 5 ] : HR4527E-521-600-14.4-NC5b
2010-07-14    purification [123.9 mg]: HR4527E-521-600-14.4-NC5b-Ni
2010-07-14    purification [37.2 mg]: HR4527E-521-600-14.4-NC5b-GF
2010-08-05     HR4527E.022 @ UGA; 0.5 ml at 0.7 mM
2010-07-21     HR4527E.020 @ Rutgers; 0.5 ml at 0.7 mM
2010-07-21     hr4527e.021 @ Rutgers; 2.5 ml at 0.7 mM
2010-01-29    construct: HR4527E-520-593-14.5
2010-03-08    expression [ 5, 5 ] : HR4527E-520-593-14.5-ss
2010-01-29    construct: HR4527E-520-593-14.6
2010-03-08    expression [ 4, 5 ] : HR4527E-520-593-14.6-ss
2010-06-09    expression [ 4, 5 ] : HR4527E-520-593-14.6-SeMa
2010-07-08    purification [131.0 mg]: HR4527E-520-593-14.6-SeMa-Ni
2010-07-08    purification [69.0 mg]: HR4527E-520-593-14.6-SeMa-GF
2010-07-12     aggregation screening [monomer,11.7 kD,53%]
2010-07-13     HR4527E.014 @ HWI; 0.45 ml at 12.18 mg/mL
2010-09-30     X-Ray structure: 3p1x (PDB)
2010-07-13     hr4527e.015 @ Rutgers; 1.4 ml at 12.18 mg/mL
2010-01-29    construct: HR4527E-526-594-14.7
2010-03-08    expression [ 5, 5 ] : HR4527E-526-594-14.7-ss
2010-01-29    construct: HR4527E-526-594-14.8
2010-03-08    expression [ 5, 5 ] : HR4527E-526-594-14.8-ss
2010-03-30    expression [ 4, 5 ] : HR4527E-526-594-14.8-SeMa
2010-04-12    purification [24.0 mg]: HR4527E-526-594-14.8-SeMa-GF
2010-04-20     aggregation screening [monomer,10.6 kD,88.2%]
2010-04-16     HR4527E.001 @ Columbia; 2 ml at 7 mg/mL
2010-04-16     HR4527E.002 @ HWI; 0.45 ml at 7 mg/mL
2010-04-16     HR4527E.003 @ Rutgers; 0.5 ml at 7 mg/mL
2010-05-05    expression [ 3, 5 ] : HR4527E-526-594-14.8-NC5a
2010-05-18    purification [26.4 mg]: HR4527E-526-594-14.8-NC5a-GF
2010-06-29     aggregation screening [monomer,11.83 kD,97.3%]
2010-05-27     HR4527E.010 @ Rutgers; 0.04 ml at 1 mM
2010-07-06    HSQC [good]: HR4527E.010.600MHz.20C
2010-05-27     hr4527e.011 @ Rutgers; 0.96 ml at 1 mM
2010-03-16    construct: HR4527E-515-572-14.15
2010-04-14    expression [ 0, 0 ] : HR4527E-515-572-14.15-ss
2010-03-16    construct: HR4527E-520-572-14.20
2010-04-14    expression [ 0, 0 ] : HR4527E-520-572-14.20-ss
2010-03-16    construct: HR4527E-520-572-14.21
2010-04-14    expression [ 0, 0 ] : HR4527E-520-572-14.21-ss
2010-03-15    construct: HR4527E-537-594-14.9
2010-04-14    expression [ 0, 0 ] : HR4527E-537-594-14.9-ss
2010-03-15    construct: HR4527E-537-594-14.10
2010-04-14    expression [ 5, 5 ] : HR4527E-537-594-14.10-ss
2010-06-08    expression [ 4, 5 ] : HR4527E-537-594-14.10-SeMa
2010-07-08    purification [45.0 mg]: HR4527E-537-594-14.10-SeMa-Ni
2010-07-08    purification [28.6 mg]: HR4527E-537-594-14.10-SeMa-GF
2010-07-12     aggregation screening [dimer,16.2 kD,86.8%]
2010-07-13     HR4527E.016 @ HWI; 0.45 ml at 9.32 mg/mL
2010-07-13     HR4527E.017 @ Columbia; 0.8 ml at 9.32 mg/mL
2010-03-15    construct: HR4527E-515-572-14.11
2010-04-14    expression [ 0, 0 ] : HR4527E-515-572-14.11-ss


Available samples:

SeMet samples
HR4527E.007 @ Columbia HR4527E.003 @ Rutgers HR4527E.016 @ HWI HR4527E.001 @ Columbia HR4527E.008 @ HWI
hr4527e.015 @ Rutgers HR4527E.014 @ HWI HR4527E.009 @ Rutgers HR4527E.005 @ HWI HR4527E.006 @ Rutgers
HR4527E.004 @ Columbia HR4527E.002 @ HWI HR4527E.017 @ Columbia


NC samples
hr4527e.019 @ Rutgers HR4527E.018 @ Rutgers


NC5 samples
hr4527e.021 @ Rutgers HR4527E.013 @ Rutgers hr4527e.011 @ Rutgers HR4527E.012 @ Rutgers HR4527E.010 @ Rutgers
HR4527E.022 @ UGA HR4527E.020 @ Rutgers