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HR4694   (BRAF)

6 targets assoicated with this target.
HR4694Cchild(149-274)selected
HR4694Achild(138-313)selected
HR4694Bchild(152-288)soluble construct with good ES score
HR4694Dchild(219-309)purified
HR4694Echild(204-285)good HSQC collected
HR4694Fchild(149-232)NMR and X-Ray structures

database refs UniProt: P15056 (BRAF_HUMAN)   |   HUGO: BRAF   |   NCBI gene: 673   |   refSeq: NP_004324.2
status selected
properties length: 766 aa   |   mass: 84.44 kD   |   pI: 7.29   |   ext: 69330   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, DrugBank-100-pctid, DrugBank-90-pctid, DrugBank-80-pctid, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:Phorbol-ester/DAG-type details   METAL:Zinc 2 (By similarity) details   56112 details   1.10.510.10 details   PF07714 details   DOMAIN:RBD details   ACT_SITE:Proton acceptor (By similarity) details   DOMAIN:Protein kinase details   BINDING:ATP (By similarity) details   SITE:Breakpoint for translocation to form KIAA1549-BRAF fusion protein details   NP_BIND:ATP (By similarity) details   METAL:Zinc 1 (By similarity) details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
There are no constructs associated with this target (HR4694).
However there are constructs associated with other targets associated with this target (below).

Domain and UniProt Features
    RBD. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:RBD
    UniProt ZN_FING:Phorbol-ester/DAG-type
    Zinc finger - Phorbol-ester/DAG-type domain_number-1
    Zinc 1. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-4
    UniProt METAL:Zinc 2 (By similarity)
    UniProt METAL:Zinc 1 (By similarity)
    Zinc 1. {ECO:0000250} domain_number-4
    Breakpoint for translocation to form domain_number-1
    Breakpoint for translocation to form domain_number-2
    UniProt SITE:Breakpoint for translocation to form KIAA1549-BRAF fusion protein
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    PFAM PF07714
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000255|PROSITE- domain_number-1
    CATH 1.10.510.10
    UniProt ACT_SITE:Proton acceptor (By similarity)
    Proton acceptor. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR4694A
    HR4694F
    HR4694C
    HR4694B
    HR4694E
    HR4694D

HR4694

       PDB seq homology



Constructs and Most Advanced Status
   
HR4694F-144-237-14.*

   
HR4694F-149-232-14.*

   
HR4694F-151-232-TEV.*

   
HR4694B-15.*

   
HR4694B-14.*

   
HR4694F-153-237-14.*

   
HR4694F-158-232-14.*

   
HR4694E-199-294-14.*

   
HR4694E-204-287-14.*

   
HR4694D-214-311-14.*

   
HR4694D-219-306-14.*



most recent record
8 years, 19 days ago --     target created

2009-08-07    target: HR4694
PLIMS data     Construct creation records
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE