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HR4694B   (BRAF)

6 targets assoicated with this target.
HR4694parentselected
HR4694Csibling(149-274)selected
HR4694Asibling(138-313)selected
HR4694Dsibling(219-309)purified
HR4694Esibling(204-285)good HSQC collected
HR4694Fsibling(149-232)NMR and X-Ray structures

database refs UniProt: P15056 (BRAF_HUMAN)   |   HUGO: BRAF   |   NCBI gene: 673   |   refSeq: NP_004324.2
status soluble construct with good ES score    work stopped (2010-06-30; End of PSI-2)
properties length: 137 aa   |   mass: 15.89 kD   |   pI: 8.81   |   ext: 15470   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T4 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Dismeta report   |   Prof PHD data   |   Pxs: 0.239   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:Phorbol-ester/DAG-type details   METAL:Zinc 2 (By similarity) details   54236 details   PF02196 details   DOMAIN:RBD details   METAL:Zinc 1 (By similarity) details   3.10.20.90 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4694B).
Display constructs associated with this target (HR4694B) and all other associated targets (HR4694, HR4694F, HR4694E, HR4694C, HR4694A, HR4694D).


Domain and UniProt Features
    RBD. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:RBD
    UniProt ZN_FING:Phorbol-ester/DAG-type
    Zinc finger - Phorbol-ester/DAG-type domain_number-1
    Zinc 1. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-4
    UniProt METAL:Zinc 2 (By similarity)
    UniProt METAL:Zinc 1 (By similarity)
    Zinc 1. {ECO:0000250} domain_number-4
    Breakpoint for translocation to form domain_number-1
    Breakpoint for translocation to form domain_number-2
    UniProt SITE:Breakpoint for translocation to form KIAA1549-BRAF fusion protein
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    PFAM PF07714
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000255|PROSITE- domain_number-1
    CATH 1.10.510.10
    UniProt ACT_SITE:Proton acceptor (By similarity)
    Proton acceptor. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR4694A
    HR4694F
    HR4694C
    HR4694B
    HR4694E
    HR4694D

HR4694

       PDB seq homology



Constructs and Most Advanced Status
   
HR4694F-144-237-14.*

   
HR4694F-149-232-14.*

   
HR4694F-151-232-TEV.*

   
HR4694B-15.*

   
HR4694B-14.*

   
HR4694F-153-237-14.*

   
HR4694F-158-232-14.*

   
HR4694E-199-294-14.*

   
HR4694E-204-287-14.*

   
HR4694D-214-311-14.*

   
HR4694D-219-306-14.*



most recent record
0 days ago --     expression [0, 0]: HR4694B-14.16-ss; expression [0, 0]: HR4694B-14.15-ss

2007-05-23    target: HR4694B     work stopped (2010-06-30; End of PSI-2)
2007-12-14    construct: HR4694B-15.1     work stopped (2010-06-30; End of PSI-2)
2008-01-24    expression [ 4, 3 ] : HR4694B-15.1-ss
2008-03-28    construct: HR4694B-14.8     work stopped (2010-06-30; End of PSI-2)
2008-08-28    construct: HR4694B-14.16     work stopped (2010-06-30; End of PSI-2)
2008-09-26    expression [ 0, 0 ] : HR4694B-14.16-ss
2008-08-28    construct: HR4694B-14.15     work stopped (2010-06-30; End of PSI-2)
2008-09-26    expression [ 0, 0 ] : HR4694B-14.15-ss