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HR4694D   (BRAF)

6 targets assoicated with this target.
HR4694parentselected
HR4694Csibling(149-274)selected
HR4694Asibling(138-313)selected
HR4694Bsibling(152-288)soluble construct with good ES score
HR4694Esibling(204-285)good HSQC collected
HR4694Fsibling(149-232)NMR and X-Ray structures

database refs UniProt: P15056 (BRAF_HUMAN)   |   HUGO: BRAF   |   NCBI gene: 673   |   refSeq: NP_004324.2
status purified    work stopped (2010-06-30; End of PSI-2)
properties length: 91 aa   |   mass: 10.72 kD   |   pI: 5.68   |   ext: 2980   |   organism: Homo sapiens
classes PSI-2, MEGA, Biomedical: Human Ubiquitin, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:Phorbol-ester/DAG-type details   METAL:Zinc 2 (By similarity) details   3.30.60.20 details   METAL:Zinc 1 (By similarity) details   57889 details   PF00130 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4694D).
Display constructs associated with this target (HR4694D) and all other associated targets (HR4694, HR4694F, HR4694E, HR4694C, HR4694A, HR4694B).


Domain and UniProt Features
    RBD. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:RBD
    UniProt ZN_FING:Phorbol-ester/DAG-type
    Zinc finger - Phorbol-ester/DAG-type domain_number-1
    Zinc 1. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-4
    UniProt METAL:Zinc 2 (By similarity)
    UniProt METAL:Zinc 1 (By similarity)
    Zinc 1. {ECO:0000250} domain_number-4
    Breakpoint for translocation to form domain_number-1
    Breakpoint for translocation to form domain_number-2
    UniProt SITE:Breakpoint for translocation to form KIAA1549-BRAF fusion protein
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    PFAM PF07714
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000255|PROSITE- domain_number-1
    CATH 1.10.510.10
    UniProt ACT_SITE:Proton acceptor (By similarity)
    Proton acceptor. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR4694A
    HR4694F
    HR4694C
    HR4694B
    HR4694E
    HR4694D

HR4694

       PDB seq homology



Constructs and Most Advanced Status
   
HR4694F-144-237-14.*

   
HR4694F-149-232-14.*

   
HR4694F-151-232-TEV.*

   
HR4694B-15.*

   
HR4694B-14.*

   
HR4694F-153-237-14.*

   
HR4694F-158-232-14.*

   
HR4694E-199-294-14.*

   
HR4694E-204-287-14.*

   
HR4694D-214-311-14.*

   
HR4694D-219-306-14.*



most recent record
4 years, 17 days ago --     purification [0.0 mg]: HR4694D-214-311-14.2-[SeMa,SeMb,SeMc]-GF

2008-12-16    target: HR4694D     work stopped (2010-06-30; End of PSI-2)
2009-09-23    construct: HR4694D-214-311-14.1     work stopped (2010-06-30; End of PSI-2)
2009-10-12    expression [ 4, 2 ] : HR4694D-214-311-14.1-ss
2009-09-23    construct: HR4694D-214-311-14.2     work stopped (2010-06-30; End of PSI-2)
2009-10-12    expression [ 5, 2 ] : HR4694D-214-311-14.2-ss
2009-11-04    expression [ 4, 2 ] : HR4694D-214-311-14.2-NC5a
2009-11-30    purification [12.1 mg]: HR4694D-214-311-14.2-NC5a-Ni
2009-12-01    purification [0.2 mg]: HR4694D-214-311-14.2-NC5a-GF
    HR4694D.001 @ Rutgers; Unknown volume and concentration
    HR4694D.002 @ Rutgers; Unknown volume and concentration
2009-11-04    expression [ 4, 2 ] : HR4694D-214-311-14.2-NC5b
2009-11-30    purification [12.1 mg]: HR4694D-214-311-14.2-NC5b-Ni
2009-12-01    purification [0.2 mg]: HR4694D-214-311-14.2-NC5b-GF
2012-09-19    expression [ 4, 5 ] : HR4694D-214-311-14.2-SeMc
2012-09-19    expression [ 4, 5 ] : HR4694D-214-311-14.2-SeMb
2012-09-19    expression [ 2, 5 ] : HR4694D-214-311-14.2-SeMa
2012-12-10    purification [0.0 mg]: HR4694D-214-311-14.2-[SeMa,SeMb,SeMc]-GF
2012-09-25    expression [ 3, 2 ] : HR4694D-214-311-14.2-SeMe
2012-09-25    expression [ 4, 5 ] : HR4694D-214-311-14.2-SeMd
2012-09-25    expression [ 3, 2 ] : HR4694D-214-311-14.2-SeMf
2009-09-23    construct: HR4694D-219-306-14.3     work stopped (2010-06-30; End of PSI-2)
2009-10-12    expression [ 0, 0 ] : HR4694D-219-306-14.3-ss
2009-09-23    construct: HR4694D-219-306-14.4     work stopped (2010-06-30; End of PSI-2)
2009-10-12    expression [ 5, 1 ] : HR4694D-219-306-14.4-ss


Available samples:

NC5 samples
HR4694D.001 @ Rutgers HR4694D.002 @ Rutgers