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HR4694E   (BRAF)

6 targets assoicated with this target.
HR4694parentselected
HR4694Csibling(149-274)selected
HR4694Asibling(138-313)selected
HR4694Bsibling(152-288)soluble construct with good ES score
HR4694Dsibling(219-309)purified
HR4694Fsibling(149-232)NMR and X-Ray structures

database refs UniProt: P15056 (BRAF_HUMAN)   |   HUGO: BRAF   |   NCBI gene: 673   |   refSeq: NP_004324.2
status good HSQC collected    work stopped (2010-06-30; End of PSI-2)
properties length: 82 aa   |   mass: 9.71 kD   |   pI: 6.31   |   ext: 13980   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:Phorbol-ester/DAG-type details   METAL:Zinc 2 (By similarity) details   3.30.60.20 details   METAL:Zinc 1 (By similarity) details   57889 details   PF00130 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4694E).
Display constructs associated with this target (HR4694E) and all other associated targets (HR4694, HR4694F, HR4694C, HR4694A, HR4694D, HR4694B).


Domain and UniProt Features
    RBD. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:RBD
    UniProt ZN_FING:Phorbol-ester/DAG-type
    Zinc finger - Phorbol-ester/DAG-type domain_number-1
    Zinc 1. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-4
    UniProt METAL:Zinc 2 (By similarity)
    UniProt METAL:Zinc 1 (By similarity)
    Zinc 1. {ECO:0000250} domain_number-4
    Breakpoint for translocation to form domain_number-1
    Breakpoint for translocation to form domain_number-2
    UniProt SITE:Breakpoint for translocation to form KIAA1549-BRAF fusion protein
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    PFAM PF07714
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000255|PROSITE- domain_number-1
    CATH 1.10.510.10
    UniProt ACT_SITE:Proton acceptor (By similarity)
    Proton acceptor. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR4694A
    HR4694F
    HR4694C
    HR4694B
    HR4694E
    HR4694D

HR4694

       PDB seq homology



Constructs and Most Advanced Status
   
HR4694F-144-237-14.*

   
HR4694F-149-232-14.*

   
HR4694F-151-232-TEV.*

   
HR4694B-15.*

   
HR4694B-14.*

   
HR4694F-153-237-14.*

   
HR4694F-158-232-14.*

   
HR4694E-199-294-14.*

   
HR4694E-204-287-14.*

   
HR4694D-214-311-14.*

   
HR4694D-219-306-14.*



most recent record
7 years, 19 days ago --     HSQC [good]

2009-09-15    target: HR4694E     work stopped (2010-06-30; End of PSI-2)
2009-11-24    construct: HR4694E-204-287-14.6     work stopped (2010-06-30; End of PSI-2)
2009-12-18    expression [ 5, 5 ] : HR4694E-204-287-14.6-ss
2009-11-20    construct: HR4694E-199-294-14.1     work stopped (2010-06-30; End of PSI-2)
2009-12-18    expression [ 4, 0 ] : HR4694E-199-294-14.1-ss
2009-11-20    construct: HR4694E-199-294-14.2     work stopped (2010-06-30; End of PSI-2)
2009-12-18    expression [ 5, 1 ] : HR4694E-199-294-14.2-ss
2009-11-20    construct: HR4694E-204-287-14.3     work stopped (2010-06-30; End of PSI-2)
2009-12-18    expression [ 5, 5 ] : HR4694E-204-287-14.3-ss
2010-01-12    expression [ 4, 5 ] : HR4694E-204-287-14.3-SeMa
2010-01-26    purification [44.1 mg]: HR4694E-204-287-14.3-SeMa-GF
2010-02-03     aggregation screening []
2010-01-29     HR4694E.001 @ Rutgers; 2 ml at 6.4 mg/mL
2010-01-29     HR4694E.002 @ HWI; 0.45 ml at 6.4 mg/mL
2010-01-29     HR4694E.003 @ Rutgers; 2.5 ml at 6.4 mg/mL
2010-05-10    HSQC [good]: HR4694E.004.600MHz.20C


Available samples:

SeMet samples
HR4694E.003 @ Rutgers HR4694E.002 @ HWI HR4694E.001 @ Rutgers