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HR4694F   (BRAF)

6 targets assoicated with this target.
HR4694parentselected
HR4694Csibling(149-274)selected
HR4694Asibling(138-313)selected
HR4694Bsibling(152-288)soluble construct with good ES score
HR4694Dsibling(219-309)purified
HR4694Esibling(204-285)good HSQC collected

database refs UniProt: P15056 (BRAF_HUMAN)   |   HUGO: BRAF   |   NCBI gene: 673   |   refSeq: NP_004324.2
status NMR and X-Ray structures
structures 2L05 (NMR)   3NY5 (Xray)  
properties length: 84 aa   |   mass: 9.50 kD   |   pI: 8.99   |   ext: 12490   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures54236 details   PF02196 details   DOMAIN:RBD details   3.10.20.90 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4694F).
Display constructs associated with this target (HR4694F) and all other associated targets (HR4694, HR4694E, HR4694C, HR4694A, HR4694D, HR4694B).


Domain and UniProt Features
    RBD. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:RBD
    UniProt ZN_FING:Phorbol-ester/DAG-type
    Zinc finger - Phorbol-ester/DAG-type domain_number-1
    Zinc 1. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-1
    Zinc 2. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-2
    Zinc 1. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-3
    Zinc 2. {ECO:0000250} domain_number-4
    UniProt METAL:Zinc 2 (By similarity)
    UniProt METAL:Zinc 1 (By similarity)
    Zinc 1. {ECO:0000250} domain_number-4
    Breakpoint for translocation to form domain_number-1
    Breakpoint for translocation to form domain_number-2
    UniProt SITE:Breakpoint for translocation to form KIAA1549-BRAF fusion protein
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    PFAM PF07714
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000255|PROSITE- domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000255|PROSITE- domain_number-1
    CATH 1.10.510.10
    UniProt ACT_SITE:Proton acceptor (By similarity)
    Proton acceptor. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR4694A
    HR4694F
    HR4694C
    HR4694B
    HR4694E
    HR4694D

HR4694

       PDB seq homology



Constructs and Most Advanced Status
   
HR4694F-144-237-14.*

   
HR4694F-149-232-14.*

   
HR4694F-149-232-21.*

   
HR4694F-151-232-TEV.*

   
HR4694B-15.*

   
HR4694B-14.*

   
HR4694F-153-237-14.*

   
HR4694F-158-232-14.*

   
HR4694E-199-294-14.*

   
HR4694E-204-287-14.*

   
HR4694D-214-311-14.*

   
HR4694D-219-306-14.*



most recent record
1 year, 7 months, 0 days ago --     purification [39.2 mg]: HR4694F-149-232-14.5-Na-Ni; purification [14.4 mg]: HR4694F-149-232-14.5-Na-GF

2009-09-15    target: HR4694F
2014-08-10    construct: HR4694F-149-232-21.1
2009-11-20    construct: HR4694F-144-237-14.1
2009-12-18    expression [ 5, 5 ] : HR4694F-144-237-14.1-ss
2009-11-20    construct: HR4694F-144-237-14.2
2009-12-18    expression [ 5, 5 ] : HR4694F-144-237-14.2-ss
2010-01-05    expression [ 5, 5 ] : HR4694F-144-237-14.2-SeMa
2010-01-12    purification [30.8 mg]: HR4694F-144-237-14.2-SeMa-GF
2010-01-20     aggregation screening [monomer,14.28 kD,78.7%|monomer,18.67 kD,12.3%]
2010-01-15     HR4694F.001 @ Rutgers; 2 ml at 8.9 mg/mL
2010-01-15     HR4694F.002 @ HWI; 0.45 ml at 8.9 mg/mL
2010-01-15     HR4694F.003 @ Rutgers; 0.7 ml at 8.9 mg/mL
2010-01-26    expression [ 5, 5 ] : HR4694F-144-237-14.2-NC5a
2010-02-25    purification [4.3 mg]: HR4694F-144-237-14.2-NC5a-Ni
2010-03-01    purification [7.0 mg]: HR4694F-144-237-14.2-NC5a-GF
2010-03-15     HR4694F.012 @ Rutgers; 0.4 ml at 0.75 mM
2010-03-19     HR4694F.011 @ Rutgers; 0.04 ml at 0.748 mM
2010-05-10    HSQC [promising]: HR4694F.011.600MHz.20C
2009-11-20    construct: HR4694F-153-237-14.3
2009-12-18    expression [ 5, 5 ] : HR4694F-153-237-14.3-ss
2009-11-20    construct: HR4694F-153-237-14.4
2009-12-18    expression [ 5, 5 ] : HR4694F-153-237-14.4-ss
2010-01-26    expression [ 4, 5 ] : HR4694F-153-237-14.4-SeMa
2010-02-08    purification [41.8 mg]: HR4694F-153-237-14.4-SeMa-GF
2010-02-22     aggregation screening []
2010-02-12     HR4694F.019 @ Rutgers; 0.8 ml at 5.2 mg/mL
2010-02-12     HR4694F.006 @ Columbia; 2 ml at 5.2 mg/mL
2010-07-14     X-Ray structure: 3ny5 (PDB)
2010-02-12     HR4694F.007 @ HWI; 0.45 ml at 5.2 mg/mL
2010-01-26    expression [ 5, 5 ] : HR4694F-153-237-14.4-NC5a
2010-02-25    purification [4.3 mg]: HR4694F-153-237-14.4-NC5a-Ni
2010-03-01    purification [9.2 mg]: HR4694F-153-237-14.4-NC5a-GF
2010-03-15     HR4694F.014 @ Rutgers; 0.1 ml at 0.84 mM
2010-03-19     HR4694F.013 @ Rutgers; 0.04 ml at 0.842 mM
2010-05-10    HSQC [good]: HR4694F.013.600MHz.20C
2009-11-20    construct: HR4694F-149-232-14.5
2009-12-18    expression [ 5, 5 ] : HR4694F-149-232-14.5-ss
2010-01-12    expression [ 4, 5 ] : HR4694F-149-232-14.5-SeMa
2010-01-12    purification [31.2 mg]: HR4694F-149-232-14.5-SeMa-GF
2010-01-27     aggregation screening [dimer,23.74 kD,87.0%]
2010-01-26     HR4694F.004 @ Columbia; 2 ml at 6.5 mg/mL
2010-01-26     HR4694F.005 @ HWI; 0.45 ml at 6.5 mg/mL
2010-03-31    expression [ 5, 5 ] : HR4694F-149-232-14.5-NCa
2010-04-05    purification [44.7 mg]: HR4694F-149-232-14.5-NCa-GF
2014-02-26     aggregation screening [monomer,12.2 kD,98.4%]
2010-04-07     HR4694F.016 @ Rutgers; 0.3 ml at 0.84 mM
2010-04-07     HR4694F.015 @ Rutgers; 2 ml at 0.46 mM
2014-03-31     HR4694F.025 @ Rutgers; 0.05 ml at 0.84 mM
2014-03-31     HR4694F.026 @ Rutgers; 0.5 ml at 1 mM
2014-03-31     HR4694F.027 @ Rutgers; 0.05 ml at 0.48 mM
2014-04-01     HR4694F.028 @ Rutgers; 0.75 ml at 0.56 mM
2010-06-30     NMR structure: 2l05 (PDB)
2010-04-06    expression [ 4, 5 ] : HR4694F-149-232-14.5-NC5b
2010-04-12    purification [25.7 mg]: HR4694F-149-232-14.5-NC5b-GF
2010-03-19     HR4694F.008 @ Rutgers; 0.04 ml at 0.689 mM
2010-05-10    HSQC [good]: HR4694F.008.600MHz.20C
2010-04-14     HR4694F.020 @ Rutgers; 1.5 ml at 1.14 mM
2010-04-21     HR4694F.021 @ UGA; 0.5 ml at 1.19 mM
2014-07-28     HR4694F.029 @ Rutgers; 0.17 ml at 12.4 mg/mL
2010-05-05    expression [ 4, 5 ] : HR4694F-149-232-14.5-NC5a
2010-03-08    purification [41.3 mg]: HR4694F-149-232-14.5-NC5a-GF
2010-03-23     aggregation screening [monomer,11.29 kD,98.3%]
2010-03-19     HR4694F.018 @ Rutgers; 3.46 ml at 0.689 mM
2010-06-17     HR4694F.023 @ Rutgers; 0.04 ml at 0.81 mM
2010-07-06    HSQC [promising]: HR4694F.023.600MHz.20C
2010-06-29     HR4694F.024 @ Rutgers; 1.75 ml at 0.81 mM
2016-02-23    expression [ 4, 5 ] : HR4694F-149-232-14.5-Na
2016-02-25    purification [39.2 mg]: HR4694F-149-232-14.5-Na-Ni
2016-02-25    purification [14.4 mg]: HR4694F-149-232-14.5-Na-GF
2016-02-26     HR4694F.030 @ Rutgers; 16 ml at 0.69 mg/mL
2009-11-20    construct: HR4694F-149-232-14.6
2009-12-18    expression [ 5, 5 ] : HR4694F-149-232-14.6-ss
2009-11-20    construct: HR4694F-158-232-14.7
2009-12-18    expression [ 5, 5 ] : HR4694F-158-232-14.7-ss
2010-02-02    expression [ 4, 5 ] : HR4694F-158-232-14.7-SeMa
2010-02-08    purification [30.0 mg]: HR4694F-158-232-14.7-SeMa-GF
2010-02-23     aggregation screening []
2010-02-19     HR4694F.009 @ Columbia; 2 ml at 4.4 mg/mL
2010-02-19     HR4694F.010 @ HWI; 0.45 ml at 4.4 mg/mL
2010-05-05    expression [ 4, 5 ] : HR4694F-158-232-14.7-NC5a
2010-06-02    purification [46.4 mg]: HR4694F-158-232-14.7-NC5a-GF
2010-06-04     HR4694F.017 @ Rutgers; 0.04 ml at 0.53 mM
2010-07-06    HSQC [good]: HR4694F.017.600MHz.20C
2010-06-04     HR4694F.022 @ Rutgers; 4.46 ml at 0.53 mM
2009-11-20    construct: HR4694F-158-232-14.8
2009-12-18    expression [ 5, 5 ] : HR4694F-158-232-14.8-ss
2014-08-10    construct: HR4694F-151-232-TEV.1
2014-08-12    expression [ 5, 5, not tested ]: HR4694F-151-232-TEV.1-Na
2014-08-14    purification [36.0 mg]: HR4694F-151-232-TEV.1-Na-Ni
2014-10-10    expression [ 4, 4, not tested ]: HR4694F-151-232-TEV.1-N14a
2014-10-10    purification [0.0 mg]: HR4694F-151-232-TEV.1-[N14a,Nb]-Ni
2014-10-10    expression [ 4, 4, not tested ]: HR4694F-151-232-TEV.1-Nb


Available samples:

SeMet samples
HR4694F.002 @ HWI HR4694F.004 @ Columbia HR4694F.001 @ Rutgers HR4694F.010 @ HWI HR4694F.009 @ Columbia
HR4694F.006 @ Columbia HR4694F.003 @ Rutgers HR4694F.019 @ Rutgers HR4694F.005 @ HWI HR4694F.007 @ HWI


Labeling not defined samples
HR4694F.030 @ Rutgers


NC samples
HR4694F.028 @ Rutgers HR4694F.026 @ Rutgers HR4694F.027 @ Rutgers HR4694F.025 @ Rutgers
HR4694F.015 @ Rutgers HR4694F.016 @ Rutgers


NC5 samples
HR4694F.014 @ Rutgers HR4694F.021 @ UGA HR4694F.023 @ Rutgers
HR4694F.022 @ Rutgers HR4694F.029 @ Rutgers HR4694F.020 @ Rutgers HR4694F.024 @ Rutgers HR4694F.017 @ Rutgers
HR4694F.013 @ Rutgers HR4694F.018 @ Rutgers HR4694F.011 @ Rutgers HR4694F.008 @ Rutgers HR4694F.012 @ Rutgers