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HR4796B   (VIM)

2 targets assoicated with this target.
HR4796Asibling(40-92)selected
HR4796parentHSQC collected

database refs UniProt: P08670 (VIME_HUMAN)   |   HUGO: VIM   |   NCBI gene: 7431   |   refSeq: NP_003371.2
status X-Ray structure
structures 3UF1 (Xray)  
properties length: 99 aa   |   mass: 11.94 kD   |   pI: 4.61   |   ext: 1490   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet T1 (Rutgers), Community Outreach, Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects Human Vimentin     HCPIN     Community Outreach    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF00038 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR4796B).
Display constructs associated with this target (HR4796B) and all other associated targets (HR4796, HR4796A).


Domain and UniProt Features
    PFAM PF00038
    [IL]-x-C-x-x-[DE] motif domain_number-1
    SuperFamily 64593
    CATH 1.20.5.170
    UniProt SITE:Stutter
    Stutter domain_number-1
    CATH 1.20.5.170


NESG targets
    HR4796A
    HR4796B

HR4796

       PDB seq homology



Constructs and Most Advanced Status
   
HR4796-1-150-15.*

   
HR4796-62-145-15.*

   
HR4796-62-140-15.*

   
HR4796-67-145-15.*

   
HR4796-67-160-15.*

   
HR4796-101-140-15.*

   
HR4796-101-145-15.*

   
HR4796-101-217-15.*

   
HR4796-101-215-15.*

   
HR4796-101-236-15.*

   
HR4796-121-150-15.*

   
HR4796B-139-256-14.*

   
HR4796B-144-251-14.*

   
HR4796B-147-304-14.*

   
HR4796-223-343-15.*

   
HR4796-223-331-15.*

   
HR4796-223-390-15.*

   
HR4796-236-390-15.*

   
HR4796-236-331-15.*

   
HR4796-236-343-15.*

   
HR4796-240-340-15.*

   
HR4796-302-430-15.*

   
HR4796-302-451-15.*

   
HR4796-302-406-15.*

   
HR4796-305-405-15.*



most recent record
5 years, 29 days ago --     X-Ray structure: 3ol1; X-Ray structure: 3uf1

2009-08-15    target: HR4796B
2009-09-15    construct: HR4796B-139-256-14.1
2009-11-04    expression [ 5, 5 ] : HR4796B-139-256-14.1-ss
2009-12-01    expression [ 5, 5 ] : HR4796B-139-256-14.1-SeMa
2009-12-08    purification [54.6 mg]: HR4796B-139-256-14.1-SeMa-GF
2009-12-15     aggregation screening [tetramer,66.8 kD,86.2%|aggregated,412.3 kD,8.1%]
2009-12-10     HR4796B.001 @ Columbia; 2 ml at 10.26 mg/mL
2009-12-10     HR4796B.002 @ HWI; 0.45 ml at 10.26 mg/mL
2009-12-10     HR4796B.003 @ Rutgers; 4.5 ml at 10.26 mg/mL
2010-02-10    expression [ 5, 5 ] : HR4796B-139-256-14.1-NC5a
2010-05-05    purification [93.1 mg]: HR4796B-139-256-14.1-NC5a-Ni
2010-05-05    purification [36.4 mg]: HR4796B-139-256-14.1-NC5a-GF
2010-06-29     HR4796B.014 @ Rutgers; 0.04 ml at 1.334 mM
2010-07-06    HSQC [poor]: HR4796B.014.600MHz.20C
2010-06-29     HR4796B.015 @ Rutgers; 0.3 ml at 1.334 mM
2009-09-15    construct: HR4796B-139-256-14.2
2009-11-04    expression [ 5, 5 ] : HR4796B-139-256-14.2-ss
2009-09-15    construct: HR4796B-144-251-14.3
2009-11-04    expression [ 5, 5 ] : HR4796B-144-251-14.3-ss
2009-12-08    expression [ 5, 5 ] : HR4796B-144-251-14.3-SeMa
2009-12-15    purification [100.1 mg]: HR4796B-144-251-14.3-SeMa-GF
2009-12-22     aggregation screening [trimer,47.5 kD,87.1%|aggregated,281.7 kD,7.1%]
2009-12-23     HR4796B.004 @ Columbia; 2 ml at 10.64 mg/mL
2011-10-31     X-Ray structure: 3ol1 (PDB)
2011-10-31     X-Ray structure: 3uf1 (PDB)
2009-12-23     HR4796B.005 @ HWI; 0.45 ml at 10.64 mg/mL
2009-12-23     HR4796B.006 @ Rutgers; 10 ml at 10.64 mg/mL
2010-02-10    expression [ 5, 5 ] : HR4796B-144-251-14.3-NC5a
2010-03-04    purification [201.1 mg]: HR4796B-144-251-14.3-NC5a-Ni
2010-03-04    purification [53.3 mg]: HR4796B-144-251-14.3-NC5a-GF
2010-03-19     HR4796B.012 @ Rutgers; 0.04 ml at 0.605 mM
2010-06-07    HSQC [poor]: HR4796B.012.600MHz.20C
2010-03-15     HR4796B.013 @ Rutgers; 2.5 ml at 0.61 mM
2009-09-15    construct: HR4796B-144-251-14.4
2009-11-04    expression [ 5, 5 ] : HR4796B-144-251-14.4-ss
2010-01-07    construct: HR4796B-147-304-14.5
2010-02-05    expression [ 5, 5 ] : HR4796B-147-304-14.5-ss
2010-01-07    construct: HR4796B-147-304-14.6
2010-02-05    expression [ 5, 5 ] : HR4796B-147-304-14.6-ss
2010-02-23    expression [ 5, 5 ] : HR4796B-147-304-14.6-SeMa
2010-03-01    purification [36.0 mg]: HR4796B-147-304-14.6-SeMa-GF
2010-03-16     aggregation screening [dimer,45.17 kD,89.8%|pentamer,103.5 kD,2.1%|octamer,167.5 kD,2.7%]
2010-03-12     HR4796B.009 @ Columbia; 2 ml at 6 mg/mL
2010-03-12     HR4796B.010 @ HWI; 0.45 ml at 6 mg/mL
2010-03-12     HR4796B.011 @ Rutgers; 1.2 ml at 6 mg/mL
2010-02-23    expression [ 5, 5 ] : HR4796B-147-304-14.6-NC5a
2010-03-03    purification [19.1 mg]: HR4796B-147-304-14.6-NC5a-GF
2010-03-12     HR4796B.007 @ Rutgers; 0.04 ml at 0.16 mM
2010-06-07    HSQC [poor]: HR4796B.007.600MHz.20C
2010-03-12     HR4796B.008 @ Rutgers; 0.9 ml at 0.16 mM


Available samples:

SeMet samples
HR4796B.011 @ Rutgers HR4796B.009 @ Columbia HR4796B.003 @ Rutgers HR4796B.005 @ HWI HR4796B.001 @ Columbia
HR4796B.002 @ HWI HR4796B.006 @ Rutgers HR4796B.004 @ Columbia HR4796B.010 @ HWI


NC5 samples
HR4796B.013 @ Rutgers
HR4796B.015 @ Rutgers HR4796B.012 @ Rutgers HR4796B.008 @ Rutgers HR4796B.014 @ Rutgers HR4796B.007 @ Rutgers