Spine target record

HR5546A target record
[nucleotide sequence] [protein sequence] [ncbi blast] [add construct]

alternative target forms:

	HR5546		full length		[ selected ]
	HR5546B		(299-393)		[ aggregation screening ]

Target status

Status: X-Ray structure
Member of: PSI-2, Biomedical: Cancer BioNet T4 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical projects.
Deposited PDB structures: 2KPW 3JT0
Structure gallery entries: 2KPW 3JT0

Target searches

Target annotation

Target comments: no comments
Annotations: Uniprot: Lamin-B1

CATH: 2.60.40.1260 -no CATH description-

Competition (top hits reported)

calculating... please wait

Target statistics

Target length: 133 aa
pI: 4.76
MW: 14.57 kD
Source organism:
Swiss-prot/trEMBL: LMNB1_HUMAN

Target bioinformatics

Pxs: 0.169
disorder prediction: ( report )
prof predictions: ( report )
Zn binding prediction (full length): ( report )

Domain families

show subsequence

classification type: MEGA
classification id: 2.60.40.1260
assigned to: not assigned to a SG group
sequence range: 9-128
competition:
calculating... please wait

Target history

most recent record
9 months, 12 days ago -- NMR structure: 2KPW

2007-05-24

target: HR5546A

2007-12-14

construct: HR5546A-15.1

2008-01-24

expression [4, 5]: HR5546A-15.1-ss

2008-01-23

expression [3, 5]: HR5546A-15.1-NC5a

2008-01-28

purification [0.5 mg]: HR5546A-15.1-NC5a-NI

2008-02-25

HSQC [promising]: HR5546A.001.600MHz.20C

2008-05-06

expression [4, 5]: HR5546A-15.1-NC5b

2008-06-05

purification [5.0 mg]: HR5546A-15.1-NC5b-Ni

2008-07-08

HSQC [promising]: HR5546A.004.600MHz.20C

2008-05-06

expression [4, 5]: HR5546A-15.1-LBa

2008-05-27

purification [1.0 mg]: HR5546A-15.1-LBa-GF

2008-03-27

construct: HR5546A-14.13

2008-03-27

construct: HR5546A-14.16

2008-08-28

construct: HR5546A-14.20

2008-09-26

expression [3, 4]: HR5546A-14.20-ss

2008-08-28

construct: HR5546A-15.2

2008-07-24

expression [3, 5]: HR5546A-15.2-LBa

2008-07-29

purification [2.2 mg]: HR5546A-15.2-LBa-Ni

2008-09-26

expression [3, 2]: HR5546A-15.2-ss

2009-03-25

construct: HR5546A-14-124-14.33

2009-04-07

expression [5, 4]: HR5546A-14-124-14.33-ss

2009-04-15

expression [4, 5]: HR5546A-14-124-14.33-SeMa

2009-04-20

purification [32.0 mg]: HR5546A-14-124-14.33-SeMa-GF

2009-09-11

Xray structure: 3JT0 (NESG gallery) (PDB)

2009-04-15

expression [4, 5]: HR5546A-14-124-14.33-NC5a

2009-04-22

purification [3.3 mg]: HR5546A-14-124-14.33-NC5a-GF

2009-05-28

aggregation screening [monomer,15.51 kD,95.6%]

2009-06-03

HSQC [good]: HR5546A.014.600MHz.20C

2009-06-02

expression [4, 5]: HR5546A-14-124-14.33-NC5b

2009-06-10

purification [116.5 mg]: HR5546A-14-124-14.33-NC5b-Ni

2009-06-11

purification [71.5 mg]: HR5546A-14-124-14.33-NC5b-GF

2009-06-09

expression [5, 5]: HR5546A-14-124-14.33-NCa

2009-06-10

purification [142.4 mg]: HR5546A-14-124-14.33-NCa-Ni

2009-06-11

purification [68.3 mg]: HR5546A-14-124-14.33-NCa-GF

2009-03-24

construct: HR5546A-14-114-14.22

2009-04-07

expression [5, 5]: HR5546A-14-114-14.22-ss

2009-03-24

construct: HR5546A-14-124-14.24

2009-04-07

expression [0, 0]: HR5546A-14-124-14.24-ss

2009-03-23

construct: HR5546A-14-114-14.28

2009-04-07

expression [5, 5]: HR5546A-14-114-14.28-ss

2009-04-15

expression [4, 5]: HR5546A-14-114-14.28-NC5a

2009-04-22

purification [3.2 mg]: HR5546A-14-114-14.28-NC5a-GF

2009-09-07

HSQC [good]: HR5546A.016.600MHz.20C

2009-10-21

NMR structure: 2KPW (NESG gallery) (PDB)

2009-04-21

expression [5, 5]: HR5546A-14-114-14.28-SeMa

2009-04-28

purification [65.5 mg]: HR5546A-14-114-14.28-SeMa-GF

2009-05-05

aggregation screening [monomer,12.96 kD,85.5%]

15N 13C samples		15N 13C(5%) samples		SeMet samples
HR5546A.019 hr5546a.018		HR5546A.022 hr5546a.020 HR5546A.005 HR5546A.014 HR5546A.023 HR5546A.015 HR5546A.017 HR5546A.001 HR5546A.004 HR5546A.021 HR5546A.016		HR5546A.013 HR5546A.010 HR5546A.008 HR5546A.012 HR5546A.011 HR5546A.009