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HR5546A   (LMNB1)

3 targets assoicated with this target.
HR5546parentselected
HR5546Csibling(31-387)expressed
HR5546Bsibling(299-393)HSQC collected and crystal hits

database refs UniProt: P20700 (LMNB1_HUMAN)   |   HUGO: LMNB1   |   NCBI gene: 4001   |   refSeq: NP_005564.1
status NMR and X-Ray structures
structures 2KPW (NMR)   3JT0 (Xray)  
properties length: 133 aa   |   mass: 14.57 kD   |   pI: 4.76   |   ext: 26470   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T4 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Prof PHD data   |   Pxs: 0.169   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures2.60.40.1260 details   74853 details   PF00932 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR5546A).
Display constructs associated with this target (HR5546A) and all other associated targets (HR5546, HR5546B, HR5546C).


Domain and UniProt Features
    PFAM PF00038
    Interchain domain_number-1
    UniProt DISULFID:Interchain
    SuperFamily 74853
    CATH 2.60.40.1260
    Nuclear localization signal domain_number-1
    UniProt MOTIF:Nuclear localization signal (Potential)
    LTD domain_number-1
    Removed in mature form. {ECO:0000305} domain_number-1
    UniProt PROPEP:Removed in mature form (Probable). /FTId=PRO_0000393945


NESG targets
    HR5546C
    HR5546B
    HR5546A

HR5546

       PDB seq homology



Constructs and Most Advanced Status
   
HR5546C-17-380-15.*

   
HR5546C-22-380-15.*

   
HR5546C-70-380-15.*

   
HR5546B-282-378-14.*

   
HR5546B-282-368-14.*

   
HR5546A-15.*

   
HR5546A-14.*

   
HR5546A-14-124-14.*

   
HR5546A-14-114-14.*



most recent record
6 years, 5 days ago --     aggregation screening [monomer,11.59 kD,92.9%]

2007-05-23    target: HR5546A
PLIMS data     Construct creation records
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE
PLIMS data     Construct small scale protein expression and soluble fraction SDS PAGE analytical scores
2007-12-14    construct: HR5546A-15.1
2008-01-24    expression [ 4, 5 ] : HR5546A-15.1-ss
2008-01-23    expression [ 3, 5 ] : HR5546A-15.1-NC5a
2008-01-28    purification [0.5 mg]: HR5546A-15.1-NC5a-NI
2008-02-04     HR5546A.001 @ Rutgers; 0.012 ml at 3.554 mM
2008-02-25    HSQC [promising]: HR5546A.001.600MHz.20C
2008-05-06    expression [ 4, 5 ] : HR5546A-15.1-NC5b
2008-06-05    purification [5.0 mg]: HR5546A-15.1-NC5b-Ni
2008-06-10     HR5546A.004 @ Rutgers; 0.012 ml at 0.176 mM
2008-07-08    HSQC [promising]: HR5546A.004.600MHz.20C
2008-07-18     HR5546A.005 @ Rutgers; 1 ml at 0.156 mM
2008-05-06    expression [ 4, 5 ] : HR5546A-15.1-LBa
2008-05-27    purification [1.0 mg]: HR5546A-15.1-LBa-GF
2008-06-02     HR5546A.002 @ Rutgers; 0.3 ml at 0.14 mM
2008-06-02     HR5546A.003 @ Rutgers; 0.08 ml at 0.14 mM
2008-03-27    construct: HR5546A-14.13
2008-03-27    construct: HR5546A-14.16
2008-08-28    construct: HR5546A-14.20
2008-09-26    expression [ 3, 4 ] : HR5546A-14.20-ss
2008-08-28    construct: HR5546A-15.2
2008-07-24    expression [ 3, 5 ] : HR5546A-15.2-LBa
2008-07-29    purification [2.2 mg]: HR5546A-15.2-LBa-Ni
2008-08-11     HR5546A.006 @ Rutgers; 1 ml at 1 mg/mL
2008-08-11     HR5546A.007 @ Rutgers; 1 ml at 1.5 mg/mL
2008-09-26    expression [ 3, 2 ] : HR5546A-15.2-ss
2009-03-25    construct: HR5546A-14-124-14.33
2009-04-07    expression [ 5, 4 ] : HR5546A-14-124-14.33-ss
2009-04-15    expression [ 4, 5 ] : HR5546A-14-124-14.33-SeMa
2009-04-20    purification [32.0 mg]: HR5546A-14-124-14.33-SeMa-GF
2010-11-23     aggregation screening [monomer,11.59 kD,92.9%]
2009-04-27     HR5546A.009 @ HWI; 0.45 ml at 10.7 mg/mL
2009-04-27     HR5546A.008 @ Columbia; 2 ml at 10.7 mg/mL
2009-09-11     X-Ray structure: 3jt0 (PDB)
2009-04-27     HR5546A.010 @ Rutgers; 1.2 ml at 10.7 mg/mL
2009-04-15    expression [ 4, 5 ] : HR5546A-14-124-14.33-NC5a
2009-04-22    purification [3.3 mg]: HR5546A-14-124-14.33-NC5a-GF
2009-05-28     aggregation screening [monomer,15.51 kD,95.6%]
2009-05-04     HR5546A.015 @ Rutgers; 0.35 ml at 0.676 mM
2009-05-04     HR5546A.014 @ Rutgers; 0.035 ml at 0.676 mM
2009-06-03    HSQC [good]: HR5546A.014.600MHz.20C
2009-06-02    expression [ 4, 5 ] : HR5546A-14-124-14.33-NC5b
2009-06-10    purification [116.5 mg]: HR5546A-14-124-14.33-NC5b-Ni
2009-06-11    purification [71.5 mg]: HR5546A-14-124-14.33-NC5b-GF
2009-06-16     hr5546a.020 @ Rutgers; 0.5 ml at 1.136 mM
2009-06-18     HR5546A.022 @ Rutgers; 2.5 ml at 1.136 mM
2009-10-21     HR5546A.023 @ Rutgers; 0.04 ml at 1.136 mM
2009-06-18     HR5546A.021 @ UGA; 0.5 ml at 1.136 mM
2009-06-09    expression [ 5, 5 ] : HR5546A-14-124-14.33-NCa
2009-06-10    purification [142.4 mg]: HR5546A-14-124-14.33-NCa-Ni
2009-06-11    purification [68.3 mg]: HR5546A-14-124-14.33-NCa-GF
2009-06-16     hr5546a.018 @ Rutgers; 0.3 ml at 1.219 mM
2009-06-18     HR5546A.019 @ Rutgers; 2.75 ml at 1.219 mM
2009-10-21     NMR structure: 2kpw (PDB)
2009-03-24    construct: HR5546A-14-114-14.22
2009-04-07    expression [ 5, 5 ] : HR5546A-14-114-14.22-ss
2009-03-24    construct: HR5546A-14-124-14.24
2009-04-07    expression [ 0, 0 ] : HR5546A-14-124-14.24-ss
2009-03-23    construct: HR5546A-14-114-14.28
2009-04-07    expression [ 5, 5 ] : HR5546A-14-114-14.28-ss
2009-04-15    expression [ 4, 5 ] : HR5546A-14-114-14.28-NC5a
2009-04-22    purification [3.2 mg]: HR5546A-14-114-14.28-NC5a-GF
2009-05-04     HR5546A.016 @ Rutgers; 0.035 ml at 0.453 mM
2009-09-07    HSQC [good]: HR5546A.016.600MHz.20C
2009-05-04     HR5546A.017 @ Rutgers; 0.35 ml at 0.453 mM
2009-04-21    expression [ 5, 5 ] : HR5546A-14-114-14.28-SeMa
2009-04-28    purification [65.5 mg]: HR5546A-14-114-14.28-SeMa-GF
2009-05-05     aggregation screening [monomer,12.96 kD,85.5%]
2009-04-30     HR5546A.011 @ Columbia; 2 ml at 10.64 mg/mL
2009-04-30     HR5546A.013 @ Rutgers; 5 ml at 10.64 mg/mL
2009-04-30     HR5546A.012 @ HWI; 0.45 ml at 10.64 mg/mL


Available samples:

LB samples
HR5546A.007 @ Rutgers HR5546A.002 @ Rutgers HR5546A.003 @ Rutgers HR5546A.006 @ Rutgers


SeMet samples
HR5546A.013 @ Rutgers
HR5546A.009 @ HWI HR5546A.012 @ HWI HR5546A.011 @ Columbia HR5546A.010 @ Rutgers HR5546A.008 @ Columbia


NC samples
HR5546A.019 @ Rutgers hr5546a.018 @ Rutgers


NC5 samples
HR5546A.014 @ Rutgers HR5546A.021 @ UGA HR5546A.022 @ Rutgers
HR5546A.016 @ Rutgers HR5546A.017 @ Rutgers HR5546A.015 @ Rutgers hr5546a.020 @ Rutgers HR5546A.005 @ Rutgers
HR5546A.004 @ Rutgers HR5546A.023 @ Rutgers HR5546A.001 @ Rutgers