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HR5546B   (LMNB1)

3 targets assoicated with this target.
HR5546parentselected
HR5546Csibling(31-387)expressed
HR5546Asibling(426-558)NMR and X-Ray structures

database refs UniProt: P20700 (LMNB1_HUMAN)   |   HUGO: LMNB1   |   NCBI gene: 4001   |   refSeq: NP_005564.1
status HSQC collected and crystal hits    work stopped (2010-06-30; End of PSI-2)
properties length: 95 aa   |   mass: 11.25 kD   |   pI: 4.77   |   ext: 2980   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet T3 (Rutgers), Biomedical: Cancer BioNet T4 (Rutgers), MEGA, Biomedical: Cancer BioNet, BioMedical, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesDISULFID:Interchain details   64593 details   1.20.5.170 details   PF00038 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR5546B).
Display constructs associated with this target (HR5546B) and all other associated targets (HR5546, HR5546A, HR5546C).


Domain and UniProt Features
    PFAM PF00038
    Interchain domain_number-1
    UniProt DISULFID:Interchain
    SuperFamily 74853
    CATH 2.60.40.1260
    Nuclear localization signal domain_number-1
    UniProt MOTIF:Nuclear localization signal (Potential)
    LTD domain_number-1
    Removed in mature form. {ECO:0000305} domain_number-1
    UniProt PROPEP:Removed in mature form (Probable). /FTId=PRO_0000393945


NESG targets
    HR5546C
    HR5546B
    HR5546A

HR5546

       PDB seq homology



Constructs and Most Advanced Status
   
HR5546C-17-380-15.*

   
HR5546C-22-380-15.*

   
HR5546C-70-380-15.*

   
HR5546B-282-378-14.*

   
HR5546B-282-368-14.*

   
HR5546A-15.*

   
HR5546A-14.*

   
HR5546A-14-124-14.*

   
HR5546A-14-114-14.*



most recent record
6 years, 27 days ago --     HSQC [poor]

2009-11-12    target: HR5546B     work stopped (2010-06-30; End of PSI-2)
2010-01-07    construct: HR5546B-282-378-14.1     work stopped (2010-06-30; End of PSI-2)
2010-02-05    expression [ 2, 4 ] : HR5546B-282-378-14.1-ss
2010-01-07    construct: HR5546B-282-378-14.2     work stopped (2010-06-30; End of PSI-2)
2010-02-05    expression [ 1, 5 ] : HR5546B-282-378-14.2-ss
2010-03-09    construct: HR5546B-282-368-14.3     work stopped (2010-06-30; End of PSI-2)
2010-04-26    expression [ 3, 5 ] : HR5546B-282-368-14.3-ss
2010-03-09    construct: HR5546B-282-368-14.4     work stopped (2010-06-30; End of PSI-2)
2010-04-26    expression [ 4, 5 ] : HR5546B-282-368-14.4-ss
2010-06-08    expression [ 5, 5 ] : HR5546B-282-368-14.4-SeMa
2010-07-06    purification [72.8 mg]: HR5546B-282-368-14.4-SeMa-GF
2010-07-12     aggregation screening [dimer,29.6 kD,92.2%]
2010-07-19     HR5546B.001 @ Columbia; 2 ml at 7.82 mg/mL
2010-07-19     HR5546B.002 @ HWI; 0.45 ml at 7.82 mg/mL
2010-07-19     HR5546B.003 @ Rutgers; 3.9 ml at 7.82 mg/mL
2011-08-04     HR5546B.006 @ Columbia; 0.1 ml at 7.82 mg/mL
2010-09-03    expression [ 4, 5 ] : HR5546B-282-368-14.4-NC5a
2010-09-13    purification [45.7 mg]: HR5546B-282-368-14.4-NC5a-Ni
2010-09-14    purification [46.2 mg]: HR5546B-282-368-14.4-NC5a-GF
2010-09-20     HR5546B.004 @ Rutgers; 0.04 ml at 0.636 mM
2010-10-04    HSQC [poor]: HR5546B.004.600MHz.20C
2010-09-20     HR5546B.005 @ Rutgers; 0.4 ml at 0.636 mM


Available samples:

SeMet samples
HR5546B.003 @ Rutgers HR5546B.002 @ HWI HR5546B.006 @ Columbia HR5546B.001 @ Columbia


NC5 samples
HR5546B.004 @ Rutgers
HR5546B.005 @ Rutgers