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HR6430A   (FUS)

1 targets assoicated with this target.
HR6430parentselected

database refs UniProt: P35637 (FUS_HUMAN)   |   HUGO: FUS   |   NCBI gene: 2521   |   refSeq: NP_001164105.1; NP_004951.1
status NMR structure
structures 2LA6 (NMR)  
properties length: 132 aa   |   mass: 14.29 kD   |   pI: 8.97   |   ext: 8480   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet, PSI-Biology
projects EPI list B     PSI:Biology Partner - EpiG     HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesSITE:Breakpoint for translocation to form FUS/TLS-CHOP oncogene details   54928 details   DOMAIN:RRM details   PF00076 details   3.30.70.330 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR6430A).
Display constructs associated with this target (HR6430A) and all other associated targets (HR6430).


Domain and UniProt Features
    UniProt SITE:Breakpoint for translocation to form chimeric FUS/ATF1 protein
    Breakpoint for translocation to form domain_number-1
    SuperFamily 54928
    Breakpoint for translocation to form domain_number-2
    UniProt SITE:Breakpoint for translocation to form FUS/TLS-CHOP oncogene
    CATH 3.30.70.330
    RRM. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:RRM
    Zinc finger - RanBP2-type. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:RanBP2-type
    PFAM PF00641


NESG targets
    HR6430A

HR6430

       PDB seq homology



Constructs and Most Advanced Status
   
HR6430A-261-370-14.*

   
HR6430A-282-370-14.*

   
HR6430A-287-370-14.*



most recent record
5 years, 10 months, 0 days ago --     purification [36.0 mg]: HR6430A-282-370-14.3-IFa-Ni; purification [23.8 mg]: HR6430A-282-370-14.3-IFa-GF

2010-04-22    target: HR6430A
2010-06-22    construct: HR6430A-261-370-14.9
2010-08-11    expression [ 3, 5 ] : HR6430A-261-370-14.9-ss
2010-11-03    expression [ 3, 5 ] : HR6430A-261-370-14.9-SeMa
2010-11-08    purification [9.6 mg]: HR6430A-261-370-14.9-SeMa-GF
2010-11-16     aggregation screening [monomer,15.85 kD,88.6%]
2010-11-16     HR6430A.005 @ Columbia; 0.4 ml at 8.2 mg/mL
2010-11-16     HR6430A.006 @ HWI; 0.45 ml at 8.2 mg/mL
2010-11-03    expression [ 3, 5 ] : HR6430A-261-370-14.9-SeMb
2010-11-08    purification [9.6 mg]: HR6430A-261-370-14.9-SeMb-GF
2010-06-22    construct: HR6430A-287-370-14.1
2010-08-11    expression [ 3, 3 ] : HR6430A-287-370-14.1-ss
2010-06-22    construct: HR6430A-287-370-14.2
2010-08-11    expression [ 3, 3 ] : HR6430A-287-370-14.2-ss
2010-06-22    construct: HR6430A-282-370-14.3
2010-08-11    expression [ 5, 5 ] : HR6430A-282-370-14.3-ss
2010-08-31    expression [ 4, 5 ] : HR6430A-282-370-14.3-SeMa
2010-09-09    purification [15.6 mg]: HR6430A-282-370-14.3-SeMa-GF
2010-09-14     aggregation screening [monomer,12.2 kD,36.6%|monomer,13.6 kD,45.5%|monomer,16.1 kD,8.2%]
2010-09-14     HR6430A.001 @ Columbia; 0.75 ml at 6.46 mg/mL
2010-09-14     HR6430A.002 @ HWI; 0.45 ml at 6.46 mg/mL
2010-09-01    expression [ 3, 5 ] : HR6430A-282-370-14.3-NC5a
2010-09-13    purification [33.3 mg]: HR6430A-282-370-14.3-NC5a-Ni
2010-09-13    purification [14.0 mg]: HR6430A-282-370-14.3-NC5a-GF
2010-10-05     aggregation screening [monomer,10.8 kD,97.8%]
2010-09-23     HR6430A.003 @ Rutgers; 0.04 ml at 1 mM
2010-10-04    HSQC [good]: HR6430A.003.600MHz.20C
2010-09-23     HR6430A.004 @ Rutgers; 0.96 ml at 1 mM
2010-10-12    expression [ 4, 5 ] : HR6430A-282-370-14.3-NCa
2010-11-19    purification [0.0 mg]: HR6430A-282-370-14.3-NCa-GF
2010-10-12    expression [ 4, 5 ] : HR6430A-282-370-14.3-NC5b
2010-11-19    purification [26.7 mg]: HR6430A-282-370-14.3-NC5b-GF
2010-11-23     HR6430A.008 @ Rutgers; 2 ml at 0.794 mM
2010-11-23     HR6430A.007 @ Rutgers; 0.5 ml at 0.794 mM
2011-02-07     HR6430A.011 @ UGA; 0.5 ml at 0.794 mM
2011-02-07     HR6430A.012 @ Rutgers; 0.5 ml at 0.794 mM
2010-12-01    expression [ 3, 5 ] : HR6430A-282-370-14.3-NCb
2010-12-08    purification [41.0 mg]: HR6430A-282-370-14.3-NCb-Ni
2010-12-08    purification [22.2 mg]: HR6430A-282-370-14.3-NCb-GF
2010-12-10     HR6430A.009 @ Rutgers; 0.3 ml at 0.83 mM
2010-12-10     HR6430A.010 @ Rutgers; 1.7 ml at 0.83 mM
2011-03-04     NMR structure: 2la6 (PDB)
2011-12-06    expression [ 3, 5 ] : HR6430A-282-370-14.3-MJ9-IF
2012-01-19    expression [ 2, 5 ] : HR6430A-282-370-14.3-IFa
2012-01-24    purification [36.0 mg]: HR6430A-282-370-14.3-IFa-Ni
2012-01-24    purification [23.8 mg]: HR6430A-282-370-14.3-IFa-GF
2012-02-06     HR6430A.013 @ Rutgers; 0.3 ml at 0.76 mM
2012-02-06     HR6430a.014 @ Rutgers; 2 ml at 0.76 mM
    HR6430A.015 @ Rutgers; 0.04 ml at 0.76 mM
2010-06-22    construct: HR6430A-282-370-14.4
2010-08-11    expression [ 5, 5 ] : HR6430A-282-370-14.4-ss
2010-06-22    construct: HR6430A-261-370-14.6
2010-08-11    expression [ 1, 0 ] : HR6430A-261-370-14.6-ss


Available samples:

SeMet samples
HR6430A.001 @ Columbia HR6430A.006 @ HWI HR6430A.005 @ Columbia HR6430A.002 @ HWI


Labeling not defined samples
HR6430A.015 @ Rutgers
HR6430A.013 @ Rutgers HR6430a.014 @ Rutgers


NC samples
HR6430A.010 @ Rutgers HR6430A.009 @ Rutgers


NC5 samples
HR6430A.012 @ Rutgers
HR6430A.011 @ UGA HR6430A.003 @ Rutgers HR6430A.008 @ Rutgers HR6430A.004 @ Rutgers HR6430A.007 @ Rutgers