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HR6470A   (NKX3-1)

1 targets assoicated with this target.
HR6470parentselected

database refs UniProt: Q99801 (NKX31_HUMAN)   |   HUGO: NKX3-1   |   NCBI gene: 4824   |   refSeq: NP_006158.2
status NMR structure
structures 2L9R (NMR)  
properties length: 92 aa   |   mass: 10.73 kD   |   pI: 10.48   |   ext: 9970   |   organism: Homo sapiens
classes PSI-2, Biomedical: Cancer BioNet, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesDNA_BIND:Homeobox details   1.10.10.60 details   46689 details   PF00046 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR6470A).
Display constructs associated with this target (HR6470A) and all other associated targets (HR6470).


Domain and UniProt Features
    CATH 1.10.10.60
    Homeobox. {ECO:0000255|PROSITE- domain_number-1
    UniProt DNA_BIND:Homeobox
    PFAM PF00046


NESG targets
    HR6470A

HR6470

       PDB seq homology



Constructs and Most Advanced Status
   
HR6470A-97-163-15.*

   
HR6470A-97-195-15.*

   
HR6470A-97-168-15.*

   
HR6470A-97-189-15.*

   
HR6470A-127-195-15.*

   
HR6470A-132-189-15.*



most recent record
6 years, 10 days ago --     HSQC [poor]

2010-06-10    target: HR6470A
2010-07-26    construct: HR6470A-97-195-15.1
2010-09-01    expression [ 1, 0 ] : HR6470A-97-195-15.1-ss
2010-07-26    construct: HR6470A-97-195-15.2
2010-09-01    expression [ 1, 1 ] : HR6470A-97-195-15.2-ss
2010-07-26    construct: HR6470A-97-189-15.3
2010-09-01    expression [ 0, 0 ] : HR6470A-97-189-15.3-ss
2010-07-26    construct: HR6470A-97-189-15.4
2010-09-01    expression [ 0, 0 ] : HR6470A-97-189-15.4-ss
2010-07-26    construct: HR6470A-97-168-15.5
2010-09-01    expression [ 4, 2 ] : HR6470A-97-168-15.5-ss
2010-07-26    construct: HR6470A-97-168-15.6
2010-09-01    expression [ 3, 5 ] : HR6470A-97-168-15.6-ss
2010-09-14    expression [ 5, 3 ] : HR6470A-97-168-15.6-SeMa
2010-09-20    purification [0.0 mg]: HR6470A-97-168-15.6-SeMa-GF
2010-09-24     HR6470A.001 @ Rutgers; 1.5 ml at 0.76 mg/mL
2010-09-21    purification [0.0 mg]: HR6470A-97-168-15.6-SeMa-Ni
2010-09-14    expression [ 5, 3 ] : HR6470A-97-168-15.6-SeMb
2010-09-20    purification [0.0 mg]: HR6470A-97-168-15.6-SeMb-GF
2010-09-21    purification [0.0 mg]: HR6470A-97-168-15.6-SeMb-Ni
2011-05-24    expression [ 5, 2 ] : HR6470A-97-168-15.6-NC5a
2011-06-01    purification [18.4 mg]: HR6470A-97-168-15.6-NC5a-Ni
2011-06-02    purification [18.8 mg]: HR6470A-97-168-15.6-NC5a-GF
2011-06-17     HR6470A.014 @ Rutgers; 0.04 ml at 0.538 mM
2011-07-22    HSQC [poor]: HR6470A.014.600MHz.20C
2011-06-17     HR6470A.015 @ Rutgers; 0.15 ml at 0.538 mM
2011-05-24    expression [ 4, 3 ] : HR6470A-97-168-15.6-NC5b
2011-06-01    purification [18.4 mg]: HR6470A-97-168-15.6-NC5b-Ni
2011-06-02    purification [18.8 mg]: HR6470A-97-168-15.6-NC5b-GF
2010-07-26    construct: HR6470A-127-195-15.7
2010-09-01    expression [ 3, 2 ] : HR6470A-127-195-15.7-ss
2010-07-26    construct: HR6470A-127-195-15.8
2010-09-01    expression [ 2, 5 ] : HR6470A-127-195-15.8-ss
2010-07-26    construct: HR6470A-97-163-15.9
2010-09-01    expression [ 4, 4 ] : HR6470A-97-163-15.9-ss
2010-09-21    expression [ 5, 3 ] : HR6470A-97-163-15.9-SeMa
2010-09-29    purification [29.4 mg]: HR6470A-97-163-15.9-SeMa-Ni
2010-10-12     HR6470A.002 @ Rutgers; 6 ml at 4.89 mg/mL
2010-09-21    expression [ 5, 2 ] : HR6470A-97-163-15.9-SeMb
2010-09-29    purification [29.4 mg]: HR6470A-97-163-15.9-SeMb-Ni
2010-10-27    expression [ 4, 5 ] : HR6470A-97-163-15.9-NC5a
2010-11-23    purification [50.0 mg]: HR6470A-97-163-15.9-NC5a-GF
2010-12-03     HR6470A.008 @ Rutgers; 0.23 ml at 0.466 mM
2010-12-01     HR6470A.007 @ Rutgers; 0.04 ml at 0.466 mM
2010-12-13    HSQC [poor]: HR6470A.007.600MHz.20C
2010-10-27    expression [ 4, 5 ] : HR6470A-97-163-15.9-NC5b
2010-07-26    construct: HR6470A-97-163-15.10
2010-09-01    expression [ 5, 1 ] : HR6470A-97-163-15.10-ss
2010-07-26    construct: HR6470A-132-189-15.11
2010-09-01    expression [ 4, 3 ] : HR6470A-132-189-15.11-ss
2010-07-26    construct: HR6470A-132-189-15.12
2013-10-03     HR6470A.019 @ Rutgers; construct sample
2010-09-01    expression [ 4, 4 ] : HR6470A-132-189-15.12-ss
2010-10-12    expression [ 3, 5 ] : HR6470A-132-189-15.12-SeMa
2010-10-20    purification [14.0 mg]: HR6470A-132-189-15.12-SeMa-GF
2010-11-03     aggregation screening [monomer,7.3 kD,92.2%]
2010-10-25     hr6470a.003 @ Rutgers; 0.758 ml at 5.24 mg/mL
2010-10-25     HR6470A.004 @ HWI; 0.45 ml at 5.24 mg/mL
2011-10-28     HR6470A.016 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-11-21     HR6470A.017 @ RAN - Toronto; 1 ml at 1 mg/mL
2012-01-30     HR6470A.018 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2012-03-14     Entered Ab Pipeline: 2012-03-14; 44 days after shipment
2013-08-15     Exited Ab Pipeline: 2013-08-15; 519 days after entering
2012-01-30     Passing antibodies: 1
2010-10-12    expression [ 3, 5 ] : HR6470A-132-189-15.12-SeMb
2010-10-20    purification [14.0 mg]: HR6470A-132-189-15.12-SeMb-GF
2010-10-27    expression [ 3, 5 ] : HR6470A-132-189-15.12-NC5a
2010-11-10    purification [44.4 mg]: HR6470A-132-189-15.12-NC5a-Ni
2010-11-16    purification [31.9 mg]: HR6470A-132-189-15.12-NC5a-GF
2011-02-01     HR6470A.011 @ Rutgers; 0.3 ml at 0.648 mM
2010-12-01     HR6470A.005 @ Rutgers; 0.04 ml at 0.648 mM
2010-12-13    HSQC [good]: HR6470A.005.600MHz.20C
2010-12-01     HR6470A.006 @ Rutgers; 2.2 ml at 0.648 mM
2011-02-07     HR6470A.012 @ UGA; 0.5 ml at 0.648 mM
2011-02-07     HR6470a.013 @ Rutgers; 0.5 ml at 0.648 mM
2010-10-27    expression [ 3, 5 ] : HR6470A-132-189-15.12-NC5b
2010-11-10    purification [44.4 mg]: HR6470A-132-189-15.12-NC5b-Ni
2010-11-16    purification [31.9 mg]: HR6470A-132-189-15.12-NC5b-GF
2011-01-05    expression [ 3, 5 ] : HR6470A-132-189-15.12-NCb
2011-01-12    purification [45.9 mg]: HR6470A-132-189-15.12-NCb-Ni
2011-01-13    purification [9.5 mg]: HR6470A-132-189-15.12-NCb-GF
2011-01-18     HR6470A.009 @ Rutgers; 0.04 ml at 0.69 mM
2011-01-18     HR6470A.010 @ Rutgers; 0.6 ml at 0.69 mM
2011-02-22     NMR structure: 2l9r (PDB)
2011-01-05    expression [ 2, 5 ] : HR6470A-132-189-15.12-NC5c
2011-01-05    expression [ 3, 5 ] : HR6470A-132-189-15.12-NCa
2011-01-12    purification [45.9 mg]: HR6470A-132-189-15.12-NCa-Ni
2011-01-13    purification [9.5 mg]: HR6470A-132-189-15.12-NCa-GF
2011-01-05    expression [ 2, 5 ] : HR6470A-132-189-15.12-NC5d


Available samples:

SeMet samples
HR6470A.017 @ RAN - Toronto HR6470A.016 @ JHU/CDI Baltimore, MD HR6470A.004 @ HWI HR6470A.018 @ CDI Mayaguez, PR hr6470a.003 @ Rutgers
HR6470A.002 @ Rutgers HR6470A.001 @ Rutgers


NC samples
HR6470A.010 @ Rutgers HR6470A.009 @ Rutgers


NC5 samples
HR6470A.006 @ Rutgers
HR6470A.005 @ Rutgers HR6470A.015 @ Rutgers HR6470A.011 @ Rutgers HR6470A.012 @ UGA HR6470a.013 @ Rutgers
HR6470A.014 @ Rutgers HR6470A.008 @ Rutgers HR6470A.007 @ Rutgers


E-mail communications associated with this target:

  2013-10-10
  2013-11-20