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HR7298F   (ZNF423)

6 targets assoicated with this target.
HR7298parentselected
HR7298Asibling(293-345)expression tested
HR7298Esibling(748-803)soluble construct
HR7298Dsibling(630-684)soluble construct with good ES score
HR7298Bsibling(1206-1268)fermentation attempted
HR7298Csibling(133-178)purified

database refs UniProt: Q2M1K9 (ZN423_HUMAN)   |   HUGO: ZNF423   |   NCBI gene: 23090   |   refSeq: NP_055884.2
status NMR structure
structures 2MDG (NMR)  
properties length: 54 aa   |   mass: 6.19 kD   |   pI: 8.89   |   ext: 1490   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:C2H2-type 22 details   PF13465 details   PF13912 details   ZN_FING:C2H2-type 23 details   3.30.160.60 details   57667 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR7298F).
Display constructs associated with this target (HR7298F) and all other associated targets (HR7298, HR7298E, HR7298B, HR7298C, HR7298A, HR7298D).


Domain and UniProt Features
    PFAM PF13912
    UniProt ZN_FING:C2H2-type 1; degenerate
    Zinc finger - C2H2-type 1; degenerate domain_number-1
    SuperFamily 57667
    CATH 3.30.160.60
    UniProt ZN_FING:C2H2-type 2
    Zinc finger - C2H2-type 2. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 57667
    CATH 3.30.160.60
    UniProt ZN_FING:C2H2-type 3
    Zinc finger - C2H2-type 3. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 57667
    CATH 3.30.160.60
    PFAM PF13465
    Zinc finger - C2H2-type 4. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 4
    SuperFamily 57667
    CATH 3.30.160.60
    PFAM PF13465
    UniProt ZN_FING:C2H2-type 5
    Zinc finger - C2H2-type 5. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 6
    Zinc finger - C2H2-type 6. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    Zinc finger - C2H2-type 7. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 7
    Zinc finger - C2H2-type 8. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 8
    Zinc finger - C2H2-type 9; degenerate domain_number-1
    UniProt ZN_FING:C2H2-type 9; degenerate
    UniProt ZN_FING:C2H2-type 10
    Zinc finger - C2H2-type 10. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 57667
    UniProt ZN_FING:C2H2-type 11
    Zinc finger - C2H2-type 11. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    UniProt ZN_FING:C2H2-type 12
    Zinc finger - C2H2-type 12. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 13; atypical
    Zinc finger - C2H2-type 13; atypical domain_number-1
    SuperFamily 57667
    UniProt ZN_FING:C2H2-type 14
    Zinc finger - C2H2-type 14. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    UniProt ZN_FING:C2H2-type 15
    Zinc finger - C2H2-type 15. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13912
    SuperFamily 57667
    PFAM PF13912
    UniProt ZN_FING:C2H2-type 16
    PFAM PF13894
    Zinc finger - C2H2-type 16. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 57667
    Zinc finger - C2H2-type 17. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    UniProt ZN_FING:C2H2-type 17
    SuperFamily 57667
    Zinc finger - C2H2-type 18. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 18
    SuperFamily 57667
    Zinc finger - C2H2-type 19. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    UniProt ZN_FING:C2H2-type 19
    SuperFamily 57667
    UniProt ZN_FING:C2H2-type 20
    Zinc finger - C2H2-type 20. {ECO:0000255|PROSITE- domain_number-1
    Zinc finger - C2H2-type 21; degenerate domain_number-1
    UniProt ZN_FING:C2H2-type 21; degenerate
    SuperFamily 57667
    SuperFamily 57667
    SuperFamily 57667
    SuperFamily 57667
    PFAM PF13912
    PFAM PF13894
    Zinc finger - C2H2-type 22. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 22
    PFAM PF13465
    CATH 3.30.160.60
    PFAM PF13912
    UniProt ZN_FING:C2H2-type 23
    Zinc finger - C2H2-type 23. {ECO:0000255|PROSITE- domain_number-1
    Zinc finger - C2H2-type 24. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 24
    Zinc finger - C2H2-type 25; degenerate domain_number-1
    UniProt ZN_FING:C2H2-type 25; degenerate
    PFAM PF13894
    Zinc finger - C2H2-type 26. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 26
    Zinc finger - C2H2-type 27. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 27
    UniProt ZN_FING:C2H2-type 28
    Zinc finger - C2H2-type 28. {ECO:0000255|PROSITE- domain_number-1
    SuperFamily 57667
    SuperFamily 57667
    Zinc finger - C2H2-type 29. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF13894
    UniProt ZN_FING:C2H2-type 29
    UniProt ZN_FING:C2H2-type 30
    Zinc finger - C2H2-type 30. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR7298C
    HR7298A
    HR7298D
    HR7298E
    HR7298F
    HR7298B

HR7298

       PDB seq homology



Constructs and Most Advanced Status
   
HR7298C-136-178-Av6HT.*

   
HR7298A-293-344-NHT.*

   
HR7298D-627-684-Av6HT.*

   
HR7298E-750-803-Av6HT.*

   
HR7298F-923-981-Av6HT.*

   
HR7298F-928-981-15.*

   
HR7298F-928-981-Av6HT.*

   
HR7298B-1204-1284-Av6HT.*



most recent record
4 years, 14 days ago --     HSQC [not scored]

2011-08-17    target: HR7298F
2011-09-13    construct: HR7298F-923-981-Av6HT.5
2011-09-29    expression [ 5, 4, not tested ]: HR7298F-923-981-Av6HT.5-ss
2011-10-04    expression [ 5, 3, not tested ]: HR7298F-923-981-Av6HT.5-SeMa
2011-10-26    purification [0.0 mg]: HR7298F-923-981-Av6HT.5-SeMa-GF
2011-10-11    expression [ 5, 4, not tested ]: HR7298F-923-981-Av6HT.5-NC5a
2011-12-06    purification [4.4 mg]: HR7298F-923-981-Av6HT.5-NC5a-Ni
2011-12-07    purification [1.3 mg]: HR7298F-923-981-Av6HT.5-NC5a-GF
2012-01-04     HR7298F.004 @ Rutgers; 0.4 ml at 0.0755896 mM
2011-10-11    expression [ 5, 3, not tested ]: HR7298F-923-981-Av6HT.5-NC5b
2011-09-12    construct: HR7298F-928-981-Av6HT.3
2011-09-29    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-ss
2011-10-18    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-SeMb
2011-10-26    purification [0.0 mg]: HR7298F-928-981-Av6HT.3-SeMb-GF
2011-10-18    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-NC5b
2011-10-25    purification [41.4 mg]: HR7298F-928-981-Av6HT.3-NC5b-Ni
2011-10-26    purification [25.1 mg]: HR7298F-928-981-Av6HT.3-NC5b-GF
2011-12-07     aggregation screening [monomer,8.76 kD,52.9%|aggregated,166.5 kD,18.2%]
2011-11-14     HR7298F.001 @ Rutgers; 0.04 ml at 0.255 mM
2011-11-21    HSQC [good]: HR7298F.001.600MHz.20C
2011-11-14     HR7298F.002 @ Rutgers; 0.1 ml at 0.255 mM
2011-10-18    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-SeMa
2011-10-26    purification [0.0 mg]: HR7298F-928-981-Av6HT.3-SeMa-GF
2011-10-18    expression [ 5, 2, not tested ]: HR7298F-928-981-Av6HT.3-NC5c
2011-10-25    purification [41.4 mg]: HR7298F-928-981-Av6HT.3-NC5c-Ni
2011-10-26    purification [25.1 mg]: HR7298F-928-981-Av6HT.3-NC5c-GF
2011-10-18    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-NC5a
2011-10-25    purification [41.4 mg]: HR7298F-928-981-Av6HT.3-NC5a-Ni
2011-10-26    purification [25.1 mg]: HR7298F-928-981-Av6HT.3-NC5a-GF
2011-12-21    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-NCb
2012-01-04    purification [12.9 mg]: HR7298F-928-981-Av6HT.3-NCb-Ni
2012-01-04    purification [3.9 mg]: HR7298F-928-981-Av6HT.3-NCb-GF
2012-01-12    purification [3.2 mg]: HR7298F-928-981-Av6HT.3-NCb-TV
2011-12-21    expression [ 5, 4, not tested ]: HR7298F-928-981-Av6HT.3-NC5e
2012-01-04    purification [11.2 mg]: HR7298F-928-981-Av6HT.3-NC5e-Ni
2012-01-04    purification [4.5 mg]: HR7298F-928-981-Av6HT.3-NC5e-GF
2012-01-12    purification [1.3 mg]: HR7298F-928-981-Av6HT.3-NC5e-TV
2011-12-21    expression [ 5, 2, not tested ]: HR7298F-928-981-Av6HT.3-NCa
2012-01-04    purification [12.9 mg]: HR7298F-928-981-Av6HT.3-NCa-Ni
2012-01-04    purification [3.9 mg]: HR7298F-928-981-Av6HT.3-NCa-GF
2012-01-12    purification [3.2 mg]: HR7298F-928-981-Av6HT.3-NCa-TV
2012-01-23     HR7298F.005 @ UGA; 0.3 ml at 0.17 mM
2012-01-26    HSQC [good]: HR7298F.005.600MHz.20C
2012-01-23     HR7298F.006 @ Rutgers; Unknown volume and concentration
2013-09-10     NMR structure: 2mdg (PDB)
2011-12-21    expression [ 5, 1, not tested ]: HR7298F-928-981-Av6HT.3-NC5d
2012-01-04    purification [11.2 mg]: HR7298F-928-981-Av6HT.3-NC5d-Ni
2012-01-04    purification [4.5 mg]: HR7298F-928-981-Av6HT.3-NC5d-GF
2012-01-12    purification [1.3 mg]: HR7298F-928-981-Av6HT.3-NC5d-TV
2012-01-23     HR7298F.007 @ Rutgers; 0.5 ml at 0.03 mM
2012-01-23     HR7298F.008 @ Rutgers; 0.05 ml at 0.03 mM
2012-07-19    expression [ 0, 0, not tested ]: HR7298F-928-981-Av6HT.3-NC5f
2012-07-19    expression [ 0, 0, not tested ]: HR7298F-928-981-Av6HT.3-NC5g
2012-08-01    expression [ 5, 4, not tested ]: HR7298F-928-981-Av6HT.3-NC5h
2012-09-12    purification [3.7 mg]: HR7298F-928-981-Av6HT.3-[NC5h,NC5i]-Ni
2012-09-12    purification [3.3 mg]: HR7298F-928-981-Av6HT.3-[NC5h,NC5i]-TV
2012-09-13    purification [1.4 mg]: HR7298F-928-981-Av6HT.3-[NC5h,NC5i]-GF
2012-09-21     HR7298F.009 @ UGA; 0.4 ml at 0.513 mM
2013-09-11    HSQC [not scored]: HR7298F.009.600MHz.20C
2012-08-01    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.3-NC5i
2012-08-16    expression [ 5, 4, not tested ]: HR7298F-928-981-Av6HT.3-NCc
2012-08-28    purification [7.8 mg]: HR7298F-928-981-Av6HT.3-NCc-Ni
2012-10-09     HR7298F.003 @ Rutgers; 5 ml at 0.09 mg/mL
2012-01-09    HSQC [good]: HR7298F.003.600MHz.20C
2012-09-12    purification [0.7 mg]: HR7298F-928-981-Av6HT.3-[NCc,NCd]-TV
2012-09-13    purification [0.3 mg]: HR7298F-928-981-Av6HT.3-[NCc,NCd]-GF
2012-08-16    expression [ 5, 4, not tested ]: HR7298F-928-981-Av6HT.3-NCd
2012-08-28    purification [7.8 mg]: HR7298F-928-981-Av6HT.3-NCd-Ni
2012-09-27    expression [ 5, 4, not tested ]: HR7298F-928-981-Av6HT.3-NCf
2012-09-27    expression [ 4, 4, not tested ]: HR7298F-928-981-Av6HT.3-NCg
2012-09-27    expression [ 5, 5, not tested ]: HR7298F-928-981-Av6HT.3-NCe
2012-10-11    purification [7.6 mg]: HR7298F-928-981-Av6HT.3-[NCe,NCf,NCg]-Ni
2012-10-11    purification [4.6 mg]: HR7298F-928-981-Av6HT.3-[NCe,NCf,NCg]-TV
2012-10-14    purification [1.0 mg]: HR7298F-928-981-Av6HT.3-[NCe,NCf,NCg]-GF
2012-11-21     HR7298F.012 @ Rutgers; 0.3 ml at 0.512081 mM
2013-05-02    expression [ 4, 0, not tested ]: HR7298F-928-981-Av6HT.3-LBa
2011-09-13    construct: HR7298F-923-981-Av6HT.6
2011-09-29    expression [ 5, 4, not tested ]: HR7298F-923-981-Av6HT.6-ss
2011-09-12    construct: HR7298F-928-981-Av6HT.4
2011-09-29    expression [ 5, 3, not tested ]: HR7298F-928-981-Av6HT.4-ss
2013-04-01    construct: HR7298F-928-981-15.7
2013-05-07    expression [ 0, 0 ] : HR7298F-928-981-15.7-ss
2013-04-01    construct: HR7298F-928-981-15.8
2013-05-07    expression [ 0, 0 ] : HR7298F-928-981-15.8-ss


Available samples:

NC samples
HR7298F.005 @ UGA HR7298F.006 @ Rutgers HR7298F.012 @ Rutgers HR7298F.003 @ Rutgers


NC5 samples
HR7298F.008 @ Rutgers
HR7298F.004 @ Rutgers HR7298F.009 @ UGA HR7298F.001 @ Rutgers HR7298F.007 @ Rutgers HR7298F.002 @ Rutgers


E-mail communications associated with this target:

  2012-09-20
  2012-09-26