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HR7614B   (INSM1)

2 targets assoicated with this target.
HR7614parentselected
HR7614Asibling(265-317)good HSQC collected

database refs UniProt: Q01101 (INSM1_HUMAN)   |   HUGO: INSM1   |   NCBI gene: 3642   |   refSeq: NP_002187.1
status NMR structure
structures 2LV2 (NMR)  
properties length: 54 aa   |   mass: 6.04 kD   |   pI: 8.66   |   ext: 2980   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:C2H2-type 5 details   ZN_FING:C2H2-type 4 details   PF13894 details   PF00096 details   57667 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR7614B).
Display constructs associated with this target (HR7614B) and all other associated targets (HR7614, HR7614A).


Domain and UniProt Features
    SuperFamily 57667
    UniProt ZN_FING:C2H2-type 1; atypical
    Zinc finger - C2H2-type 1; atypical domain_number-1
    CATH 3.30.160.60
    Zinc finger - C2H2-type 2. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF00096
    UniProt ZN_FING:C2H2-type 2
    Zinc finger - C2H2-type 3. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 3
    PFAM PF13894
    SuperFamily 57667
    SuperFamily 57667
    PFAM PF00096
    UniProt ZN_FING:C2H2-type 4
    Zinc finger - C2H2-type 4. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 5
    PFAM PF13894
    Zinc finger - C2H2-type 5. {ECO:0000255|PROSITE- domain_number-1


NESG targets
    HR7614A
    HR7614B

HR7614

       PDB seq homology



Constructs and Most Advanced Status
   
HR7614A-263-300-15.*

   
HR7614A-263-319-15.*

   
HR7614B-424-497-15.*

   
HR7614B-429-493-15.*

   
HR7614B-432-497-15.*



most recent record
5 years, 29 days ago --     NMR structure: 2lv2

2011-03-16    target: HR7614B
2011-05-05    construct: HR7614B-424-497-15.9
2011-05-25    expression [ 4, 5 ] : HR7614B-424-497-15.9-ss
2011-05-05    construct: HR7614B-424-497-15.10
2013-10-03     HR7614B.027 @ Rutgers; construct sample
2011-05-25    expression [ 4, 5 ] : HR7614B-424-497-15.10-ss
2011-06-14    expression [ 5, 5 ] : HR7614B-424-497-15.10-SeMa
2011-07-20    purification [22.4 mg]: HR7614B-424-497-15.10-SeMa-GF
2011-08-12     aggregation screening [monomer,7.86 kD,88.2%]
2011-07-26     HR7614B.010 @ Rutgers; 2 ml at 5.5 mg/mL
2011-07-26     HR7614B.011 @ HWI; 0.45 ml at 5.5 mg/mL
2011-06-15    expression [ 5, 5 ] : HR7614B-424-497-15.10-NC5a
2011-07-13    purification [86.7 mg]: HR7614B-424-497-15.10-NC5a-Ni
2011-07-13    purification [70.4 mg]: HR7614B-424-497-15.10-NC5a-GF
2011-08-11     aggregation screening [monomer,10.6 kD,70.2%]
2011-07-24     HR7614B.003 @ Rutgers; 0.04 ml at 1.645 mM
2011-08-11    HSQC [good]: HR7614B.003.600MHz.20C
2011-09-29     HR7614B.004 @ Rutgers; 0.44 ml at 1.645 mM
2011-09-29     HR7614B.012 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-09-29     HR7614B.013 @ RAN - Toronto; 1 ml at 1 mg/mL
2011-09-29     HR7614B.014 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2012-03-14     Entered Ab Pipeline: 2012-03-14; 167 days after shipment
2013-04-22     Exited Ab Pipeline: 2013-04-22; 404 days after entering
2011-09-29     Passing antibodies: 1
2011-09-29     HR7614B.015 @ Rutgers; 1 ml at 1 mg/mL
2012-07-19     HR7614B.026 @ UGA; 0.5 ml at 1.645 mM
2011-08-17    expression [ 4, 5 ] : HR7614B-424-497-15.10-NC5b
2011-09-06    purification [129.5 mg]: HR7614B-424-497-15.10-NC5b-Ni
2011-09-06    purification [28.9 mg]: HR7614B-424-497-15.10-NC5b-GF
2011-10-05     HR7614B.023 @ Miami; 0.5 ml at 1.25 mM
2011-09-15    HSQC [good]: HR7614B.023.600MHz.25C
2011-10-05     HR7614B.024 @ Rutgers; 1 ml at 1.25 mM
2011-08-24    expression [ 5, 5 ] : HR7614B-424-497-15.10-NCa
2011-09-06    purification [134.6 mg]: HR7614B-424-497-15.10-NCa-Ni
2011-09-06    purification [34.4 mg]: HR7614B-424-497-15.10-NCa-GF
2011-10-05     HR7614B.021 @ Miami; 0.3 ml at 0.69 mM
2011-09-15    HSQC [good]: HR7614B.021.600MHz.25C
2011-10-05     HR7614B.022 @ Miami; 1.1 ml at 0.69 mM
2012-06-27     NMR structure: 2lv2 (PDB)
2011-05-09    construct: HR7614B-429-493-15.21
2011-05-25    expression [ 4, 5 ] : HR7614B-429-493-15.21-ss
2011-05-09    construct: HR7614B-429-493-15.22
2011-05-25    expression [ 4, 5 ] : HR7614B-429-493-15.22-ss
2011-06-14    expression [ 5, 4 ] : HR7614B-429-493-15.22-SeMa
2011-07-20    purification [3.2 mg]: HR7614B-429-493-15.22-SeMa-GF
2011-08-12     aggregation screening [#VALUE!,. kD,0%]
2011-07-26     HR7614B.009 @ Rutgers; 0.45 ml at 1.3 mg/mL
2011-06-15    expression [ 4, 3 ] : HR7614B-429-493-15.22-NC5a
2011-07-13    purification [126.4 mg]: HR7614B-429-493-15.22-NC5a-Ni
2011-07-13    purification [33.3 mg]: HR7614B-429-493-15.22-NC5a-GF
2011-07-24     HR7614B.007 @ Rutgers; 0.04 ml at 0.897 mM
2011-08-11    HSQC [good]: HR7614B.007.600MHz.20C
2011-09-29     HR7614B.008 @ Rutgers; 1 ml at 1 mg/mL
2011-09-29     HR7614B.019 @ Rutgers; 1 ml at 1 mg/mL
2011-09-29     HR7614B.020 @ Rutgers; 1 ml at 1 mg/mL
2011-05-09    construct: HR7614B-432-497-15.29
2011-05-25    expression [ 4, 5 ] : HR7614B-432-497-15.29-ss
2011-05-09    construct: HR7614B-432-497-15.30
2011-05-25    expression [ 4, 5 ] : HR7614B-432-497-15.30-ss
2011-06-14    expression [ 5, 4 ] : HR7614B-432-497-15.30-SeMa
2011-06-20    purification [29.0 mg]: HR7614B-432-497-15.30-SeMa-GF
2011-06-28     aggregation screening [dimer,15.47 kD,80.8%]
2011-06-24     HR7614B.001 @ HWI; 0.45 ml at 5.9 mg/mL
2011-06-24     HR7614B.002 @ Rutgers; 0.2 ml at 5.9 mg/mL
2011-06-15    expression [ 4, 5 ] : HR7614B-432-497-15.30-NC5a
2011-07-13    purification [146.8 mg]: HR7614B-432-497-15.30-NC5a-Ni
2011-07-13    purification [63.4 mg]: HR7614B-432-497-15.30-NC5a-GF
2011-07-24     HR7614B.005 @ Rutgers; 0.04 ml at 0.794 mM
2011-08-11    HSQC [good]: HR7614B.005.600MHz.20C
2011-09-29     HR7614B.006 @ Rutgers; 0.07 ml at 0.794 mM
2011-09-29     HR7614B.016 @ Rutgers; 1 ml at 1 mg/mL
2011-09-29     HR7614B.017 @ Rutgers; 1 ml at 1 mg/mL
2011-09-29     HR7614B.018 @ Rutgers; 1 ml at 1 mg/mL


Available samples:

SeMet samples
HR7614B.010 @ Rutgers HR7614B.009 @ Rutgers HR7614B.001 @ HWI HR7614B.011 @ HWI HR7614B.002 @ Rutgers


NC samples
HR7614B.021 @ Miami HR7614B.022 @ Miami


NC5 samples
HR7614B.003 @ Rutgers HR7614B.024 @ Rutgers HR7614B.008 @ Rutgers
HR7614B.005 @ Rutgers HR7614B.007 @ Rutgers HR7614B.026 @ UGA HR7614B.023 @ Miami HR7614B.017 @ Rutgers
HR7614B.006 @ Rutgers HR7614B.020 @ Rutgers HR7614B.013 @ RAN - Toronto HR7614B.018 @ Rutgers HR7614B.014 @ CDI Mayaguez, PR
HR7614B.012 @ JHU/CDI Baltimore, MD HR7614B.016 @ Rutgers HR7614B.015 @ Rutgers HR7614B.004 @ Rutgers HR7614B.019 @ Rutgers


E-mail communications associated with this target:

  2012-07-20
  2012-07-31
  2013-10-10
  2013-11-20