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HR7992A   (IKZF4)

4 targets assoicated with this target.
HR7992parentselected
HR7992Bsibling(528-578)expressed
HR7992Csibling(159-272)soluble construct with good ES score
HR7992Dsibling(210-260)soluble construct with excellent ES score

database refs UniProt: Q9H2S9 (IKZF4_HUMAN)   |   HUGO: IKZF4   |   NCBI gene: 64375   |   refSeq: NP_071910.3
status NMR structure
structures 2MA7 (NMR)  
properties length: 62 aa   |   mass: 6.89 kD   |   pI: 9.61   |   ext: 0   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, Biomedical: Transcription Factor, PSI-Biology
projects HCPIN     Transcription Factor    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: CH type found (data) CHDE type found (data)
domain signaturesZN_FING:C2H2-type 1 details   PF13465 details   3.30.160.60 details   ZN_FING:C2H2-type 2 details   57667 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR7992A).
Display constructs associated with this target (HR7992A) and all other associated targets (HR7992, HR7992D, HR7992C, HR7992B).


Domain and UniProt Features
    SuperFamily 57667
    Zinc finger - C2H2-type 1. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 1
    CATH 3.30.160.60
    PFAM PF13465
    CATH 3.30.160.60
    SuperFamily 57667
    Zinc finger - C2H2-type 2. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 2
    PFAM PF13465
    CATH 3.30.160.60
    SuperFamily 57667
    Zinc finger - C2H2-type 3. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 3
    CATH 3.30.160.60
    UniProt ZN_FING:C2H2-type 4
    Zinc finger - C2H2-type 4. {ECO:0000255|PROSITE- domain_number-1
    PFAM PF00096
    CTBP-binding motif PEDLA domain_number-1
    UniProt MOTIF:CTBP-binding motif PEDLA
    SuperFamily 57667
    SuperFamily 57667
    UniProt ZN_FING:C2H2-type 5
    Zinc finger - C2H2-type 5. {ECO:0000255|PROSITE- domain_number-1
    Zinc finger - C2H2-type 6. {ECO:0000255|PROSITE- domain_number-1
    UniProt ZN_FING:C2H2-type 6


NESG targets
    HR7992A
    HR7992C
    HR7992D
    HR7992B

HR7992

       PDB seq homology



Constructs and Most Advanced Status
   
HR7992A-150-221-15.*

   
HR7992A-150-232-15.*

   
HR7992A-150-246-15.*

   
HR7992A-155-216-TEV.*

   
HR7992A-155-239-15.*

   
HR7992A-155-216-15.*

   
HR7992C-155-272-Av6HT.*

   
HR7992A-155-216-15.7-15TEV.*

   
HR7992C-159-283-Av6HT.*

   
HR7992C-159-272-Av6HT.*

   
HR7992D-197-260-Av6HT.*

   
HR7992D-208-283-Av6HT.*

   
HR7992D-210-260-Av6HT.*

   
HR7992B-513-585-15.*

   
HR7992B-518-585-15.*

   
HR7992B-523-585-15.*

   
HR7992B-528-585-15.*



most recent record
3 years, 22 days ago --     NMR structure: 2ma7

2011-03-16    target: HR7992A
2011-05-25    construct: HR7992A-150-232-15.3
2011-06-13    expression [ 0, 0 ] : HR7992A-150-232-15.3-ss
2011-05-25    construct: HR7992A-150-232-15.4
2011-06-13    expression [ 0, 0 ] : HR7992A-150-232-15.4-ss
2011-05-25    construct: HR7992A-150-246-15.5
2011-06-13    expression [ 0, 0 ] : HR7992A-150-246-15.5-ss
2011-05-25    construct: HR7992A-155-216-15.7
2013-10-03     HR7992A.016 @ Rutgers; construct sample
2011-06-13    expression [ 3, 5 ] : HR7992A-155-216-15.7-ss
2011-06-29    expression [ 4, 5 ] : HR7992A-155-216-15.7-SeMa
2011-07-14    purification [0.0 mg]: HR7992A-155-216-15.7-SeMa-GF
2011-07-26     aggregation screening [Aggregated,597.5 kD,13.9%]
2011-08-03     HR7992A.003 @ Rutgers; 0.35 ml at 10 mg/mL
2011-08-03     HR7992A.002 @ Columbia; 2 ml at 10 mg/mL
2011-08-03     HR7992A.001 @ HWI; 0.45 ml at 10 mg/mL
2011-06-29    expression [ 3, 5 ] : HR7992A-155-216-15.7-NC5c
2011-07-13    purification [33.5 mg]: HR7992A-155-216-15.7-NC5c-Ni
2011-07-13    purification [27.7 mg]: HR7992A-155-216-15.7-NC5c-GF
2011-07-24     HR7992A.004 @ Rutgers; 0.04 ml at 1.5 mM
2011-08-11    HSQC [good]: HR7992A.004.600MHz.20C
2011-09-29     HR7992A.006 @ JHU/CDI Baltimore, MD; 1 ml at 1 mg/mL
2011-09-29     HR7992A.007 @ RAN - Toronto; 1 ml at 1 mg/mL
2011-09-29     HR7992A.008 @ CDI Mayaguez, PR; 1 ml at 1 mg/mL
2011-09-29     HR7992A.009 @ Rutgers; 1 ml at 1 mg/mL
2011-06-29    expression [ 4, 5 ] : HR7992A-155-216-15.7-SeMb
2011-07-14    purification [0.0 mg]: HR7992A-155-216-15.7-SeMb-GF
2011-06-29    expression [ 3, 5 ] : HR7992A-155-216-15.7-NC5a
2011-07-13    purification [33.5 mg]: HR7992A-155-216-15.7-NC5a-Ni
2011-07-13    purification [27.7 mg]: HR7992A-155-216-15.7-NC5a-GF
2011-06-29    expression [ 3, 5 ] : HR7992A-155-216-15.7-NC5b
2011-07-13    purification [33.5 mg]: HR7992A-155-216-15.7-NC5b-Ni
2011-07-13    purification [27.7 mg]: HR7992A-155-216-15.7-NC5b-GF
2011-08-17    expression [ 3, 5 ] : HR7992A-155-216-15.7-NCa
2011-08-24    purification [45.8 mg]: HR7992A-155-216-15.7-NCa-Ni
2011-08-25    purification [4.6 mg]: HR7992A-155-216-15.7-NCa-GF
2011-09-01     HR7992A.010 @ Rutgers; 0.3 ml at 1 mM
2011-09-15    HSQC [good]: HR7992A.010.600MHz.25C
2014-07-01     NMR structure: 2ma7 (PDB)
2011-08-17    expression [ 3, 5 ] : HR7992A-155-216-15.7-NC5d
2011-08-24    purification [43.6 mg]: HR7992A-155-216-15.7-NC5d-Ni
2011-08-25    purification [4.4 mg]: HR7992A-155-216-15.7-NC5d-GF
2011-09-01     HR7992A.011 @ Buffalo; 0.5 ml at 1 mM
2011-11-21    HSQC [promising]: HR7992A.011.600MHz.20C
2011-09-01     HR7992A.012 @ Rutgers; 0.2 ml at 1 mM
2011-09-15    HSQC [good]: HR7992A.012.600MHz.25C
2011-12-14    expression [ 3, 5 ] : HR7992A-155-216-15.7-NC5e
2011-12-15    purification [30.0 mg]: HR7992A-155-216-15.7-NC5e-Ni
2011-12-15    purification [18.3 mg]: HR7992A-155-216-15.7-NC5e-GF
2011-12-20     HR7992A.013 @ Buffalo; 0.5 ml at 0.5 mM
2011-12-20     HR7992A.014 @ Rutgers; 0.5 ml at 0.5 mM
2011-12-20     HR7992A.005 @ UGA; 1 ml at 0.5 mM
2011-12-14    expression [ 3, 5 ] : HR7992A-155-216-15.7-NC5f
2011-12-15    purification [30.0 mg]: HR7992A-155-216-15.7-NC5f-Ni
2011-12-15    purification [18.3 mg]: HR7992A-155-216-15.7-NC5f-GF
2013-04-04    expression [ 3, 1 ] : HR7992A-155-216-15.7-LBa
2011-05-25    construct: HR7992A-155-216-15.8
2011-06-13    expression [ 3, 4 ] : HR7992A-155-216-15.8-ss
2011-05-25    construct: HR7992A-155-239-15.9
2011-06-13    expression [ 0, 0 ] : HR7992A-155-239-15.9-ss
2011-05-25    construct: HR7992A-155-239-15.10
2011-06-13    expression [ 0, 0 ] : HR7992A-155-239-15.10-ss
2011-05-25    construct: HR7992A-150-221-15.18
2011-06-13    expression [ 0, 0 ] : HR7992A-150-221-15.18-ss
2011-05-25    construct: HR7992A-150-221-15.12
2011-06-13    expression [ 0, 0 ] : HR7992A-150-221-15.12-ss
2011-05-25    construct: HR7992A-150-246-15.16
2011-06-13    expression [ 0, 0 ] : HR7992A-150-246-15.16-ss
2013-09-10    construct: HR7992A-155-216-TEV.21
2013-09-23    expression [ -1, -1 ] : HR7992A-155-216-TEV.21-ss
2013-09-10    construct: HR7992A-155-216-TEV.22
2013-09-23    expression [ 5, 5 ] : HR7992A-155-216-TEV.22-ss
2012-08-23    construct: HR7992A-155-216-15.7-15TEV.1
2012-09-05    expression [ 5, 4 ] : HR7992A-155-216-15.7-15TEV.1-NC5a
2012-08-30    expression [ 5, 5 ] : HR7992A-155-216-15.7-15TEV.1-NCa
2012-09-25    purification [8.7 mg]: HR7992A-155-216-15.7-15TEV.1-NCa-Ni
2012-09-25    purification [4.8 mg]: HR7992A-155-216-15.7-15TEV.1-NCa-TV
2012-09-26    purification [1.0 mg]: HR7992A-155-216-15.7-15TEV.1-NCa-GF
2012-10-10     HR7992A.015 @ Buffalo; 0.3 ml at 0.3 mM
2012-08-23    construct: HR7992A-155-216-15.7-15TEV.2
2012-09-05    expression [ 5, 4 ] : HR7992A-155-216-15.7-15TEV.2-NC5a
2012-08-30    expression [ 5, 5 ] : HR7992A-155-216-15.7-15TEV.2-NCa


Available samples:

SeMet samples
HR7992A.001 @ HWI HR7992A.002 @ Columbia HR7992A.003 @ Rutgers


NC samples
HR7992A.010 @ Rutgers HR7992A.015 @ Buffalo


NC5 samples
HR7992A.014 @ Rutgers HR7992A.006 @ JHU/CDI Baltimore, MD HR7992A.004 @ Rutgers HR7992A.013 @ Buffalo HR7992A.005 @ UGA
HR7992A.008 @ CDI Mayaguez, PR HR7992A.012 @ Rutgers HR7992A.009 @ Rutgers HR7992A.007 @ RAN - Toronto HR7992A.011 @ Buffalo


E-mail communications associated with this target:

  2012-10-18
  2012-10-19
  2013-10-10
  2013-11-20