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HR8574A   (GAS7)

2 targets assoicated with this target.
HR8574parentselected
HR8574Bsibling(200-421)soluble construct with good ES score

database refs UniProt: O60861 (GAS7_HUMAN)   |   HUGO: GAS7   |   NCBI gene: 8522
status NMR structure
structures 2LX7 (NMR)  
properties length: 73 aa   |   mass: 8.06 kD   |   pI: 5.05   |   ext: 19480   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF00018 details   50044 details   DOMAIN:SH3 details   2.30.30.40 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR8574A).
Display constructs associated with this target (HR8574A) and all other associated targets (HR8574, HR8574B).


Domain and UniProt Features
    SH3. {ECO:0000255|PROSITE- domain_number-1
    CATH 2.30.30.40
    UniProt DOMAIN:SH3
    WW. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:WW
    F-BAR. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:FCH
    SuperFamily 103657
    PFAM PF00611


NESG targets
    HR8574A
    HR8574B

HR8574

       PDB seq homology



Constructs and Most Advanced Status
   
HR8574A-1-60-Av6HT.*

   
HR8574A-1-73-Av6HT.*

   
HR8574B-192-421-Av6HT.*

   
HR8574B-192-467-Av6HT.*

   
HR8574B-199-467-Av6HT.*

   
HR8574B-199-437-Av6HT.*

   
HR8574B-199-421-Av6HT.*



most recent record
5 years, 15 days ago --     NMR structure: 2lx7

2011-11-01    target: HR8574A
2011-11-16    construct: HR8574A-1-73-Av6HT.13
2011-12-09    expression [ 4, 5, not tested ]: HR8574A-1-73-Av6HT.13-ss
2012-01-04    expression [ 5, 4, not tested ]: HR8574A-1-73-Av6HT.13-SeMa
2012-01-17    purification [10.4 mg]: HR8574A-1-73-Av6HT.13-SeMa-GF
2012-01-25     aggregation screening [monomer,9.98 kD,84.8%|tetramer,42.45 kD,7.6%]
2012-01-23     HR8574A.003 @ HWI; 0.45 ml at 7.7 mg/mL
    HR8574A.004 @ Rutgers; 2.5 ml at 0.4 mg/mL
2012-01-11    expression [ 4, 5, not tested ]: HR8574A-1-73-Av6HT.13-NC5a
2012-03-05    purification [11.4 mg]: HR8574A-1-73-Av6HT.13-NC5a-Ni
2012-03-07    purification [6.3 mg]: HR8574A-1-73-Av6HT.13-NC5a-TV
2012-03-28     HR8574A.007 @ Rutgers; 0.04 ml at 0.695 mM
2012-04-13    HSQC [good]: HR8574A.007.600MHz.25C
2012-03-29     HR8574A.008 @ Rutgers; 0.4 ml at 1.12 mM
2012-05-21     HR8574A.010 @ Rutgers; 0.05 ml at 5.76 mg/mL
2012-05-22    expression [ 5, 4, not tested ]: HR8574A-1-73-Av6HT.13-NCa
2012-05-30    expression [ 4, 1, not tested ]: HR8574A-1-73-Av6HT.13-NC5b
2011-11-16    construct: HR8574A-1-60-Av6HT.2
2011-12-09    expression [ 5, 4, not tested ]: HR8574A-1-60-Av6HT.2-ss
2011-12-20    expression [ 5, 3, not tested ]: HR8574A-1-60-Av6HT.2-NC5a
2011-12-30    purification [2.1 mg]: HR8574A-1-60-Av6HT.2-NC5a-Ni
2011-12-31    purification [2.0 mg]: HR8574A-1-60-Av6HT.2-NC5a-GF
2012-01-27     aggregation screening [monomer,11.36 kD,75.4%|aggregated,142 kD,14.2%]
2012-01-02     HR8574A.001 @ Rutgers; 0.04 ml at 0.503 mM
2012-01-24    HSQC [good]: HR8574A.001.600MHz.20C
2012-01-04     HR8574A.002 @ Rutgers; 0.3 ml at 0.502871 mM
2011-12-20    expression [ 5, 3, not tested ]: HR8574A-1-60-Av6HT.2-SeMa
2012-02-01    expression [ 5, 3, not tested ]: HR8574A-1-60-AV6HT.2-NCa
2012-02-07    purification [10.1 mg]: HR8574A-1-60-AV6HT.2-NCa-Ni
2012-02-07    purification [2.1 mg]: HR8574A-1-60-AV6HT.2-NCa-GF
2012-05-03     HR8574A.005 @ Miami; 0.3 ml at 0.26232 mM
2012-08-15     NMR structure: 2lx7 (PDB)
2012-02-07    purification [9.0 mg]: HR8574A-1-60-Av6HT.2-NCa-TV
2012-02-01    expression [ 5, 3, not tested ]: HR8574A-1-60-AV6HT.2-NC5b
2012-02-07    purification [9.7 mg]: HR8574A-1-60-AV6HT.2-NC5b-Ni
2012-02-07    purification [2.4 mg]: HR8574A-1-60-AV6HT.2-NC5b-GF
2012-05-03     HR8574A.006 @ Miami; 0.5 ml at 0.452002 mM
2012-02-07    purification [9.6 mg]: HR8574A-1-60-Av6HT.2-NC5b-TV
2012-03-06    expression [ 4, 3, not tested ]: HR8574A-1-60-Av6HT.2-NCb
2012-03-20    purification [1.0 mg]: HR8574A-1-60-Av6HT.2-NCb-Ni
2012-03-20    purification [2.2 mg]: HR8574A-1-60-Av6HT.2-NCb-TV
2012-04-10     HR8574A.009 @ Buffalo; 0.3 ml at 0.33 mM
2012-03-29    HSQC [good]: HR8574A.009.600MHz.20C
2012-03-06    expression [ 4, 3, not tested ]: HR8574A-1-60-Av6HT.2-NCc
2012-03-20    purification [1.0 mg]: HR8574A-1-60-Av6HT.2-NCc-Ni
2012-03-20    purification [2.2 mg]: HR8574A-1-60-Av6HT.2-NCc-TV
2011-11-16    construct: HR8574A-1-73-Av6HT.4
2011-12-09    expression [ 0, 0, not tested ]: HR8574A-1-73-Av6HT.4-ss
2011-11-16    construct: HR8574A-1-60-Av6HT.1
2011-12-09    expression [ 5, 4, not tested ]: HR8574A-1-60-Av6HT.1-ss


Available samples:

SeMet samples
HR8574A.003 @ HWI HR8574A.004 @ Rutgers


NC samples
HR8574A.005 @ Miami HR8574A.009 @ Buffalo


NC5 samples
HR8574A.007 @ Rutgers
HR8574A.008 @ Rutgers HR8574A.006 @ Miami HR8574A.002 @ Rutgers HR8574A.010 @ Rutgers HR8574A.001 @ Rutgers


E-mail communications associated with this target:

  2012-04-26