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HR8998C   (AP4B1)

3 targets assoicated with this target.
HR8998Asibling(12-583)expression tested
HR8998parentexpressed
HR8998Bsibling(7-573)expressed

database refs UniProt: Q9Y6B7 (AP4B1_HUMAN)   |   HUGO: AP4B1   |   NCBI gene: 10717
status NMR structure
structures 2MJ7 (NMR)  
properties length: 120 aa   |   mass: 13.58 kD   |   pI: 5.09   |   ext: 19480   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures3.30.310.10 details   55711 details   PF09066 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR8998C).
Display constructs associated with this target (HR8998C) and all other associated targets (HR8998, HR8998B, HR8998A).


Domain and UniProt Features
    SuperFamily 48371
    PFAM PF01602
    CATH 1.25.10.10
    SuperFamily 48371
    CATH 1.25.10.10


NESG targets
    HR8998B
    HR8998A
    HR8998C

HR8998

       PDB seq homology



Constructs and Most Advanced Status
   
HR8998-1-541-15.*

   
HR8998-1-580-15.*

   
HR8998-1-556-15.*

   
HR8998A-1-583-Av6HT.*

   
HR8998-1-594-15.*

   
HR8998B-1-573-Av6HT.*

   
HR8998B-1-541-Av6HT.*

   
HR8998B-7-573-Av6HT.*

   
HR8998C-595-739-15.*

   
HR8998C-605-739-15.*

   
HR8998C-610-739-15.*

   
HR8998C-620-739-15.*



most recent record
3 years, 2 days ago --     NMR structure: 2mj7

2012-03-06    target: HR8998C
2012-08-08    construct: HR8998C-620-739-15.8
2012-08-28    expression [ 5, 2 ] : HR8998C-620-739-15.8-ss
2012-08-08    construct: HR8998C-610-739-15.6
2012-08-28    expression [ 5, 2 ] : HR8998C-610-739-15.6-ss
2013-01-15    expression [ 5, 3 ] : HR8998C-610-739-15.6-SeMb
2013-01-15    expression [ 5, 2 ] : HR8998C-610-739-15.6-SeMa
2013-02-11    purification [12.6 mg]: HR8998C-610-739-15.6-[SeMa,SeMb]-GF
2013-02-26     aggregation screening [dimer,29.27 kD,62.5%|tetramer,60.78 kD,28.21%]
2013-02-14     HR8998C.004 @ Rutgers; 0.55 ml at 9.55 mg/mL
2013-02-14     HR8998C.003 @ HWI; 0.45 ml at 9.55 mg/mL
2013-01-15    expression [ 5, 3 ] : HR8998C-610-739-15.6-NC5a
2013-02-04    purification [19.4 mg]: HR8998C-610-739-15.6-[NC5a,NC5b]-Ni
2013-02-06    purification [6.1 mg]: HR8998C-610-739-15.6-[NC5a,NC5b]-GF
2013-05-20     aggregation screening [monomer,16.36 kD,81.2%]
2013-02-08     HR8998C.002 @ Buffalo; 0.25 ml at 0.76 mM
2013-02-08     HR8998C.001 @ Rutgers; 0.04 ml at 0.76 mM
2013-03-20    HSQC [good]: HR8998C.001.600MHz.20C
2013-01-15    expression [ 5, 4 ] : HR8998C-610-739-15.6-NC5b
2013-02-20    expression [ 5, 2 ] : HR8998C-610-739-15.6-NCa
2013-03-18    purification [21.6 mg]: HR8998C-610-739-15.6-[NCa,NCb]-Ni
2013-03-18    purification [7.3 mg]: HR8998C-610-739-15.6-[NCa,NCb]-GF
2013-03-21     HR8998C.005 @ Buffalo; 0.3 ml at 0.17 mM
2013-12-26     NMR structure: 2mj7 (PDB)
2013-02-26    expression [ 5, 2 ] : HR8998C-610-739-15.6-NCb
2013-06-26    expression [ 5, 0 ] : HR8998C-610-739-15.6-NC5c
2013-07-30    purification [14.9 mg]: HR8998C-610-739-15.6-NC5c-Ni
2013-07-31    purification [0.5 mg]: HR8998C-610-739-15.6-NC5c-GF
2013-08-06     HR8998C.007 @ Rutgers; 0.5 ml at 0.048 mM
2013-06-26    expression [ 5, 0 ] : HR8998C-610-739-15.6-NCc
2013-07-30    purification [8.5 mg]: HR8998C-610-739-15.6-NCc-Ni
2013-07-31    purification [0.2 mg]: HR8998C-610-739-15.6-NCc-GF
2013-08-06     HR8998C.006 @ Rutgers; 0.5 ml at 0.022 mM
2013-07-09    expression [ 5, 2 ] : HR8998C-610-739-15.6-NC5d
2013-08-14    purification [22.4 mg]: HR8998C-610-739-15.6-[NC5d,NC5e]-Ni
2013-08-14    purification [4.4 mg]: HR8998C-610-739-15.6-[NC5d,NC5e]-GF
2013-08-19     HR8998C.009 @ Buffalo; 0.5 ml at 0.367 mM
2013-08-07    expression [ 5, 5 ] : HR8998C-610-739-15.6-NCd
2013-08-14    purification [24.3 mg]: HR8998C-610-739-15.6-[NCd,NCe]-Ni
2013-08-14    purification [3.4 mg]: HR8998C-610-739-15.6-[NCd,NCe]-GF
2013-08-22    purification [3.1 mg]: HR8998C-610-739-15.6-[NCd,NCe]-Ni2
2013-08-19     HR8998C.008 @ Buffalo; 0.3 ml at 0.21 mM
2013-08-07    expression [ 5, 5 ] : HR8998C-610-739-15.6-NCe
2013-08-07    expression [ 5, 5 ] : HR8998C-610-739-15.6-NC5e
2012-08-08    construct: HR8998C-620-739-15.7
2012-08-28    expression [ 5, 2 ] : HR8998C-620-739-15.7-ss
2012-08-08    construct: HR8998C-610-739-15.5
2012-08-28    expression [ 5, 2 ] : HR8998C-610-739-15.5-ss
2012-08-09    construct: HR8998C-595-739-15.1
2012-08-22    expression [ 5, 2 ] : HR8998C-595-739-15.1-ss
2012-08-09    construct: HR8998C-595-739-15.2
2012-08-22    expression [ 5, 2 ] : HR8998C-595-739-15.2-ss
2012-08-09    construct: HR8998C-605-739-15.3
2012-08-22    expression [ 5, 2 ] : HR8998C-605-739-15.3-ss
2012-08-09    construct: HR8998C-605-739-15.4
2012-08-22    expression [ 5, 2 ] : HR8998C-605-739-15.4-ss


Available samples:

SeMet samples
HR8998C.004 @ Rutgers HR8998C.003 @ HWI


NC samples
HR8998C.005 @ Buffalo HR8998C.006 @ Rutgers HR8998C.008 @ Buffalo


NC5 samples
HR8998C.007 @ Rutgers HR8998C.001 @ Rutgers HR8998C.002 @ Buffalo HR8998C.009 @ Buffalo


E-mail communications associated with this target:

  2013-02-27
  2013-03-01
  2013-06-12
  2013-06-12
  2013-08-29
  2013-09-01