JavaScript Menu Courtesy of Milonic.com
[nucleotide sequence]     [protein sequence]     [ncbi BLAST]     [add construct]     [add ligand annotation]

HR9129A   (ARHGAP10)

1 targets assoicated with this target.
HR9129parentexpressed

database refs UniProt: A1A4S6 (RHG10_HUMAN)   |   HUGO: ARHGAP10   |   NCBI gene: 79658
status NMR structure
structures 2MIO (NMR)  
properties length: 56 aa   |   mass: 6.31 kD   |   pI: 5.73   |   ext: 8480   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, PSI-Biology
projects HCPIN    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF00018 details   50044 details   2.30.30.40 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR9129A).
Display constructs associated with this target (HR9129A) and all other associated targets (HR9129).


Domain and UniProt Features
    SuperFamily 103657
    BAR domain_number-1
    UniProt DOMAIN:BAR
    PH. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:PH
    Rho-GAP. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Rho-GAP
    CATH 1.10.555.10
    PFAM PF00620
    SH3. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:SH3


NESG targets
    HR9129A

HR9129

       PDB seq homology



Constructs and Most Advanced Status
   
HR9129-1-577-15.*

   
HR9129-1-591-15.*

   
HR9129A-728-786-15.*



most recent record
3 years, 3 days ago --     aggregation screening [monomer,7.95 kD,86.0%]

2012-01-12    target: HR9129A
2012-04-09    construct: HR9129A-728-786-15.1
2012-06-29    expression [ 4, 2 ] : HR9129A-728-786-15.1-ss
2012-04-09    construct: HR9129A-728-786-15.2
2012-06-29    expression [ 4, 4 ] : HR9129A-728-786-15.2-ss
2013-02-26    expression [ 4, 5 ] : HR9129A-728-786-15.2-SeMa
2013-03-20    purification [27.0 mg]: HR9129A-728-786-15.2-SeMa-GF
2013-04-02     aggregation screening [#VALUE!,. kD,%]
2013-03-22     HR9129A.001 @ HWI; 0.45 ml at 5.1 mg/mL
2013-03-22     HR9129A.002 @ Rutgers; 2 ml at 5.1 mg/mL
2013-02-26    expression [ 3, 5 ] : HR9129A-728-786-15.2-NC5a
2013-06-05    purification [43.6 mg]: HR9129A-728-786-15.2-NC5a-Ni
2013-06-10    purification [24.6 mg]: HR9129A-728-786-15.2-NC5a-GF
2013-06-12     HR9129A.003 @ Rutgers; 0.04 ml at 0.63 mM
2013-07-12    HSQC [good]: HR9129A.003.600MHz.20C
2013-06-12     HR9129A.004 @ Rutgers; 2.46 ml at 0.63 mM
2013-10-08     HR9129A.007 @ Buffalo; 0.5 ml at 0.63 mM
2013-08-07    expression [ 5, 5 ] : HR9129A-728-786-15.2-NCa
2013-08-14    purification [46.2 mg]: HR9129A-728-786-15.2-NCa-Ni
2013-08-14    purification [30.6 mg]: HR9129A-728-786-15.2-NCa-GF
2013-08-19     HR9129A.005 @ Buffalo; 0.3 ml at 0.72 mM
2013-08-19     HR9129A.006 @ Rutgers; 3.7 ml at 0.72 mM
2014-01-15     NMR structure: 2mio (PDB)
2013-08-07    expression [ 5, 5 ] : HR9129A-728-786-15.2-SeMb
2014-02-18    purification [12.0 mg]: HR9129A-728-786-15.2-SeMb-Ni
2014-02-19    purification [3.2 mg]: HR9129A-728-786-15.2-SeMb-GF
2014-02-24     aggregation screening [monomer,7.95 kD,86.0%]
2014-02-20     HR9129A.008 @ HWI; 0.5 ml at 4.43 mg/mL
2014-02-20     HR9129A.009 @ Rutgers; 2.5 ml at 4.43 mg/mL


Available samples:

SeMet samples
HR9129A.001 @ HWI HR9129A.008 @ HWI HR9129A.002 @ Rutgers HR9129A.009 @ Rutgers


NC samples
HR9129A.006 @ Rutgers
HR9129A.005 @ Buffalo


NC5 samples
HR9129A.007 @ Buffalo HR9129A.003 @ Rutgers HR9129A.004 @ Rutgers


E-mail communications associated with this target:

  2013-04-08
  2013-04-08
  2013-04-11
  2013-04-11
  2013-10-09
  2014-03-05
  2014-03-07