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HR9151A   (MAST1)

2 targets assoicated with this target.
HR9151parentselected
HR9151Bsibling(227-693)fermented with good ES score

database refs UniProt: Q9Y2H9 (MAST1_HUMAN)   |   HUGO: MAST1   |   NCBI gene: 22983
status NMR structure
structures 2M9X (NMR)  
properties length: 101 aa   |   mass: 11.65 kD   |   pI: 5.36   |   ext: 5960   |   organism: Homo sapiens
classes Biomedical: Cancer BioNet, PSI-Biology
projects HCPIN     Human brain proteins    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures140482 details   PF08926 details   1.20.1480.20 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (HR9151A).
Display constructs associated with this target (HR9151A) and all other associated targets (HR9151, HR9151B).


Domain and UniProt Features
    PFAM PF08926
    SuperFamily 56112
    Protein kinase. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:Protein kinase
    UniProt NP_BIND:ATP (By similarity)
    ATP. {ECO:0000250|UniProtKB:Q9BXM7, domain_number-1
    UniProt BINDING:ATP (By similarity)
    Binding - ATP. {ECO:0000250|UniProtKB:Q9BXM7, domain_number-1
    CATH 1.10.510.10
    Proton acceptor domain_number-1
    UniProt ACT_SITE:Proton acceptor (By similarity)
    UniProt DOMAIN:AGC-kinase C-terminal
    AGC-kinase C-terminal domain_number-1
    PDZ. {ECO:0000255|PROSITE- domain_number-1
    UniProt DOMAIN:PDZ


NESG targets
    HR9151A
    HR9151B

HR9151

       PDB seq homology



Constructs and Most Advanced Status
   
HR9151A-175-287-15.*

   
HR9151A-175-328-15.*

   
HR9151A-187-287-15.*

   
HR9151A-187-328-15.*

   
HR9151B-214-670-15.*

   
HR9151B-214-678-15.*

   
HR9151B-214-688-15.*



most recent record
3 years, 26 days ago --     HSQC [not scored]; HSQC [not scored]

2012-01-12    target: HR9151A
2012-04-17    construct: HR9151A-175-287-15.1
2012-05-15    expression [ 4, 0 ] : HR9151A-175-287-15.1-ss
2012-04-18    construct: HR9151A-187-287-15.9
2012-05-15    expression [ 0, 0 ] : HR9151A-187-287-15.9-ss
2012-04-17    construct: HR9151A-175-287-15.2
2012-05-15    expression [ 4, 0 ] : HR9151A-175-287-15.2-ss
2012-04-17    construct: HR9151A-187-328-15.7
2012-05-15    expression [ 5, 4 ] : HR9151A-187-328-15.7-ss
2012-05-23    expression [ 5, 2 ] : HR9151A-187-328-15.7-SeMa
2012-05-23    expression [ 5, 2 ] : HR9151A-187-328-15.7-NC5a
2012-07-13    purification [14.8 mg]: HR9151A-187-328-15.7-NC5a-GF
2012-07-23     HR9151A.005 @ Rutgers; 0.04 ml at 0.359 mM
2012-09-05    HSQC [poor]: HR9151A.005.600MHz.20C
2012-07-23     HR9151A.006 @ Rutgers; 0.3 ml at 0.359 mM
2012-04-17    construct: HR9151A-175-328-15.4
2012-05-15    expression [ 3, 2 ] : HR9151A-175-328-15.4-ss
2012-04-17    construct: HR9151A-187-328-15.8
2012-05-15    expression [ 5, 4 ] : HR9151A-187-328-15.8-ss
2012-04-17    construct: HR9151A-187-287-15.5
2012-05-15    expression [ 5, 4 ] : HR9151A-187-287-15.5-ss
2012-05-23    expression [ 5, 4 ] : HR9151A-187-287-15.5-SeMa
2012-07-10    purification [19.0 mg]: HR9151A-187-287-15.5-SeMa-GF
2012-07-17     aggregation screening [monomer,13.47 kD,59.0%|monomer,13.48 kD,32.4%]
2012-07-18     HR9151A.003 @ HWI; 0.45 ml at 8.04 mg/mL
2012-07-18     HR9151A.004 @ HWI; 0.45 ml at 10.09 mg/mL
2012-07-09    purification [89.4 mg]: HR9151A-187-287-15.5-SeMa-Ni
2012-05-23    expression [ 5, 4 ] : HR9151A-187-287-15.5-NC5a
2012-06-26    purification [46.5 mg]: HR9151A-187-287-15.5-NC5a-Ni
2012-06-26    purification [13.8 mg]: HR9151A-187-287-15.5-NC5a-GF
2012-07-24     HR9151A.001 @ Rutgers; 0.04 ml at 0.8 mM
2012-09-05    HSQC [good]: HR9151A.001.600MHz.20C
2012-07-06     HR9151A.002 @ Rutgers; 2.96 ml at 0.8 mM
2012-10-03    expression [ 4, 5 ] : HR9151A-187-287-15.5-NCa
2012-10-11    purification [34.6 mg]: HR9151A-187-287-15.5-NCa-Ni
2012-10-14    purification [19.3 mg]: HR9151A-187-287-15.5-NCa-GF
2012-11-13     HR9151A.007 @ Buffalo; 0.3 ml at 0.507886 mM
2014-04-28    HSQC [not scored]: HR9151A.007.600MHz.20C
2012-11-21     HR9151A.008 @ Rutgers; 0.6 ml at 0.508 mM
2012-11-26     HR9151A.011 @ Buffalo; 0.3 ml at 0.508 mM
2013-06-20     NMR structure: 2m9x (PDB)
2012-10-03    expression [ 4, 5 ] : HR9151A-187-287-15.5-NC5b
2012-10-11    purification [34.4 mg]: HR9151A-187-287-15.5-NC5b-Ni
2012-10-14    purification [18.5 mg]: HR9151A-187-287-15.5-NC5b-GF
2012-11-13     HR9151A.009 @ Buffalo; 0.55 ml at 0.387584 mM
2014-04-28    HSQC [not scored]: HR9151A.009.600MHz.20C
2012-11-21     HR9151A.010 @ Buffalo; 0.3 ml at 0.388 mM
2012-04-17    construct: HR9151A-175-328-15.3
2012-05-15    expression [ 3, 2 ] : HR9151A-175-328-15.3-ss


Available samples:

SeMet samples
HR9151A.004 @ HWI HR9151A.003 @ HWI


NC samples
HR9151A.011 @ Buffalo HR9151A.008 @ Rutgers HR9151A.007 @ Buffalo


NC5 samples
HR9151A.006 @ Rutgers HR9151A.009 @ Buffalo HR9151A.001 @ Rutgers HR9151A.010 @ Buffalo HR9151A.005 @ Rutgers
HR9151A.002 @ Rutgers


E-mail communications associated with this target:

  2012-07-19
  2012-07-19
  2012-11-13
  2012-11-27
  2012-11-28
  2013-02-07