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LkR112   (NT07LI_0805)

database refs UniProt: E3YVT8 (E3YVT8_LISIN)
status NMR and X-Ray structures
structures 2KPP (NMR)   3LD7 (Xray)  
properties length: 140 aa   |   mass: 15.60 kD   |   pI: 7.82   |   ext: 8480   |   organism: Listeria innocua
classes PSI-2, META Genomic, META Genomic: Gut, PSI-Biology
projects CS-Rosetta using deuterated samples     Community Outreach    
best PDB match calculating ...
informatics Prof PHD data   |   DISOPRED2 data   |   Pxs: 0.204   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)   |   TM helices (details)
domain signaturesPF07009 details   2.60.320.10 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    PFAM PF07009
    CATH 2.60.320.10

LkR112

       PDB seq homology



Constructs and Most Advanced Status
   
LkR112-36-127-21.*

   
LkR112-36-122-21.*

   
LkR112-36-140-21.*



most recent record
7 years, 29 days ago --     purification [7.1 mg]: LkR112-36-127-21.11-DNC-ILV-GF; purification [10.7 mg]: LkR112-36-127-21.11-DNC-ILV-Ni

2007-08-29    target: LkR112
2009-06-01    construct: LkR112-36-127-21.10
2009-06-17    expression [ 4, 5 ] : LkR112-36-127-21.10-ss
2009-06-01    construct: LkR112-36-127-21.11
2009-06-17    expression [ 4, 5 ] : LkR112-36-127-21.11-ss
2009-06-23    expression [ 5, 5 ] : LkR112-36-127-21.11-NC5a
2009-07-09    purification [64.0 mg]: LkR112-36-127-21.11-NC5a-GF
2009-07-15     LkR112.004 @ Rutgers; 0.035 ml at 1.584 mM
2009-08-30    HSQC [good]: LkR112.004.600MHz.25C
2009-07-15     lkr112.005 @ Rutgers; 2.6 ml at 1.584 mM
2009-07-21    expression [ 5, 5 ] : LkR112-36-127-21.11-SeMa
2009-08-03    purification [48.4 mg]: LkR112-36-127-21.11-SeMa-GF
2009-10-20     aggregation screening [monomer,14.34 kD,76%|heptamer,82.1 kD,6%]
2009-08-06     LkR112.011 @ HWI; 0.45 ml at 11.2 mg/mL
2009-08-06     LkR112.012 @ Rutgers; 2 ml at 11.2 mg/mL
2009-08-06     LkR112.010 @ Columbia; 2 ml at 11.2 mg/mL
2010-01-12     X-Ray structure: 3LD7 (PDB)
2010-06-16    expression [ 4, 5 ] : LkR112-36-127-21.11-DNC-ILV
2010-06-29    purification [7.1 mg]: LkR112-36-127-21.11-DNC-ILV-GF
2010-07-01     LkR112.019 @ Rutgers; 0.1 ml at 1.17 mM
2010-07-01     lkr112.020 @ Rutgers; 0.05 ml at 1.17 mM
2012-11-26     LkR112.022 @ Buffalo; 0.3 ml at 1.17 mM
2014-11-18     LkR112.021 @ Rutgers; 0.05 ml at 1.17 mM
2010-06-29    purification [10.7 mg]: LkR112-36-127-21.11-DNC-ILV-Ni
2009-06-05    construct: LkR112-36-140-21.21
2009-06-17    expression [ 5, 5 ] : LkR112-36-140-21.21-ss
2009-06-23    expression [ 5, 5 ] : LkR112-36-140-21.21-SeMa
2009-07-04    purification [44.0 mg]: LkR112-36-140-21.21-SeMa-GF
2009-07-07     aggregation screening [monomer,12.83 kD,99.2%]
2009-07-01     LkR112.001 @ Columbia; 2 ml at 6.3 mg/mL
2009-07-01     LkR112.002 @ HWI; 0.45 ml at 6.3 mg/mL
2009-07-01     LkR112.003 @ Rutgers; 1.8 ml at 6.3 mg/mL
2009-06-23    expression [ 5, 5 ] : LkR112-36-140-21.21-NC5a
2009-07-09    purification [46.7 mg]: LkR112-36-140-21.21-NC5a-GF
2009-07-15     LkR112.008 @ Rutgers; 0.035 ml at 1.32 mM
2009-08-30    HSQC [good]: LkR112.008.600MHz.25C
2009-07-15     lkr112.009 @ Rutgers; 2.5 ml at 1.32 mM
2009-08-03    expression [ 5, 5 ] : LkR112-36-140-21.21-NCa
2009-08-10    purification [52.4 mg]: LkR112-36-140-21.21-NCa-GF
2009-09-09     aggregation screening [monomer,12.62 kD,92.8%]
2009-08-11     LkR112.013 @ Rutgers; 0.3 ml at 0.851 mM
2009-08-11     lkr112.014 @ Rutgers; 3.7 ml at 0.851 mM
2010-03-29     LkR112.018 @ UGA; 0.5 ml at 0.851 mM
2009-10-18     NMR structure: 2kpp (PDB)
2009-08-03    expression [ 5, 5 ] : LkR112-36-140-21.21-NC5b
2009-08-10    purification [30.8 mg]: LkR112-36-140-21.21-NC5b-GF
2009-08-11     LkR112.015 @ Rutgers; 0.5 ml at 0.887 mM
2009-09-02     LkR112.017 @ UGA; 0.5 ml at 0.887 mM
2009-08-11     lkr112.016 @ Rutgers; 2.5 ml at 0.887 mM
2009-06-05    construct: LkR112-36-140-21.22
2009-06-17    expression [ 5, 5 ] : LkR112-36-140-21.22-ss
2009-06-01    construct: LkR112-36-122-21.5
2009-06-17    expression [ 2, 5 ] : LkR112-36-122-21.5-ss
2009-06-01    construct: LkR112-36-122-21.6
2009-06-17    expression [ 4, 5 ] : LkR112-36-122-21.6-ss
2009-06-23    expression [ 5, 5 ] : LkR112-36-122-21.6-NC5a
2009-07-09    purification [21.4 mg]: LkR112-36-122-21.6-NC5a-GF
2009-07-15     LkR112.006 @ Rutgers; 0.035 ml at 1.408 mM
2009-08-30    HSQC [good]: LkR112.006.600MHz.25C
2009-07-15     lkr112.007 @ Rutgers; 1.1 ml at 1.408 mM


Available samples:

SeMet samples
LkR112.003 @ Rutgers LkR112.001 @ Columbia LkR112.011 @ HWI LkR112.012 @ Rutgers LkR112.002 @ HWI
LkR112.010 @ Columbia


Labeling not defined samples
LkR112.021 @ Rutgers lkr112.020 @ Rutgers LkR112.022 @ Buffalo LkR112.019 @ Rutgers


NC samples
LkR112.013 @ Rutgers LkR112.018 @ UGA lkr112.014 @ Rutgers


NC5 samples
LkR112.004 @ Rutgers LkR112.017 @ UGA
lkr112.009 @ Rutgers lkr112.007 @ Rutgers LkR112.006 @ Rutgers lkr112.016 @ Rutgers lkr112.005 @ Rutgers
LkR112.015 @ Rutgers LkR112.008 @ Rutgers


E-mail communications associated with this target:

  2012-11-27
  2012-11-28