JavaScript Menu Courtesy of Milonic.com
[nucleotide sequence]     [protein sequence]     [ncbi BLAST]     [add construct]     [add ligand annotation]

MiR12   (calU16)

database refs UniProt: Q8KNE9 (Q8KNE9_MICEC)
status NMR and X-Ray structures
structures 2LUZ (NMR)   4FPW (Xray)  
properties length: 182 aa   |   mass: 20.11 kD   |   pI: 4.53   |   ext: 30480   |   organism: Micromonospora echinospora
classes Community Outreach, Community Outreach: Enzyme Natural Product Discovery, PSI-Biology
projects Enz of Nat. Prod. Biosyn.     Community Outreach    
best PDB match calculating ...
informatics Dismeta report   |   Prof PHD data   |   DISOPRED2 data   |   Pxs: 0.282   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures55961 details   3.30.530.20 details   PF08327 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    SuperFamily 55961
    CATH 3.30.530.20
    PFAM PF08327

MiR12

       PDB seq homology



Constructs and Most Advanced Status
   
MiR12-1-158-15.*

   
MiR12-15.*

   
MiR12-1-164-15.*

   
MiR12-6-181-15.*

   
MiR12-6-164-15.*

   
MiR12-11-158-15.*

   
MiR12-11-181-15.*



most recent record
5 years, 5 months, 0 days ago --     X-Ray structure: 4FPW; NMR structure: 2luz

2011-01-10    target: MiR12
2011-03-23    construct: MiR12-15.1
2011-04-26    expression [ 5, 5 ] : MiR12-15.1-ss
2011-07-19    expression [ 5, 5 ] : MiR12-15.1-SeM-R1
2011-07-27    purification [42.0 mg]: MiR12-15.1-SeM-R1-GF
2011-08-01     aggregation screening [monomer,22.1 kD,90.5%]
2011-08-03     MiR12.001 @ Columbia; 2 ml at 6.4 mg/mL
2012-06-22     X-Ray structure: 4FPW (PDB)
2011-08-03     MiR12.002 @ HWI; 0.45 ml at 6.4 mg/mL
2011-08-03     MiR12.003 @ Rutgers; 1 ml at 6.4 mg/mL
2012-05-01     MiR12.013 @ Phillips; 0.5 ml at 6.4 mg/mL
2011-07-19    expression [ 5, 5 ] : MiR12-15.1-NC5a
2011-08-08    purification [66.2 mg]: MiR12-15.1-NC5a-Ni
2011-08-09    purification [36.3 mg]: MiR12-15.1-NC5a-GF
2011-08-15     MiR12.004 @ Rutgers; 0.04 ml at 0.916 mM
2011-09-15    HSQC [good]: MiR12.004.600MHz.25C
2011-08-15     MiR12.005 @ Rutgers; 1.5 ml at 0.916 mM
2011-10-12    expression [ 4, 5 ] : MiR12-15.1-NCa
2011-10-27    purification [32.3 mg]: MiR12-15.1-NCa-Ni
2011-10-27    purification [52.4 mg]: MiR12-15.1-NCa-GF
2011-11-01     MiR12.007 @ Rutgers; 1.3 ml at 0.89 mM
2011-11-01     mir12.006 @ Miami; 0.3 ml at 0.89 mM
2011-11-11    HSQC [good]: MiR12.006.600MHz.25C
2012-02-01     MiR12.011 @ Miami; 0.5 ml at 0.89 mM
2012-03-05     MiR12.012 @ UGA; 0.4 ml at 0.89 mM
2012-06-22     NMR structure: 2luz (PDB)
2011-10-12    expression [ 5, 5 ] : MiR12-15.1-NC5b
2011-10-27    purification [32.3 mg]: MiR12-15.1-NC5b-Ni
2011-10-27    purification [47.4 mg]: MiR12-15.1-NC5b-GF
2011-11-01     mir12.009 @ Rutgers; 0.5 ml at 0.96 mM
2011-11-01     mir12.008 @ Miami; 0.5 ml at 0.96 mM
2011-11-11    HSQC [good]: MiR12.008.600MHz.25C
2011-12-06     MiR12.010 @ UGA; 1 ml at 0.96 mM
2011-11-02    expression [ 5, 5 ] : MiR12-15.1-NC5c
2011-11-02    expression [ 5, 5 ] : MiR12-15.1-NCb
2012-04-17    expression [ 4, 5 ] : MiR12-15.1-LBa
2012-05-21    purification [32.4 mg]: MiR12-15.1-LBa-Ni
2012-05-22    purification [49.4 mg]: MiR12-15.1-LBa-GF
2012-05-30     aggregation screening [monomer,19.63 kD,92.2%]
2012-05-30     MiR12.016 @ Rutgers; 0.45 ml at 10.43 mg/mL
2012-05-30     mir12.017 @ Miami; 2 ml at 10.43 mg/mL
2012-05-30     MiR12.018 @ Rutgers; 2 ml at 10.43 mg/mL
2011-03-23    construct: MiR12-15.2
2011-04-26    expression [ 5, 5 ] : MiR12-15.2-ss
2011-03-23    construct: MiR12-11-181-15.5
2011-04-26    expression [ 3, 5 ] : MiR12-11-181-15.5-ss
2012-04-25    expression [ 4, 4 ] : MiR12-11-181-15.5-SeM-R1
2012-05-21    purification [32.0 mg]: MiR12-11-181-15.5-SeM-R1-Ni
2012-05-22    purification [20.8 mg]: MiR12-11-181-15.5-SeM-R1-GF
2012-05-30     aggregation screening [monomer,24.7 kD,52.8%|monomer,26.89 kD,33.3%]
2012-05-30     MiR12.019 @ HWI; 0.45 ml at 8.8 mg/mL
2012-05-30     MiR12.020 @ Rutgers; 1.1 ml at 8.8 mg/mL
2011-03-23    construct: MiR12-11-181-15.6
2011-04-26    expression [ 3, 5 ] : MiR12-11-181-15.6-ss
2011-03-23    construct: MiR12-1-164-15.7
2011-04-26    expression [ 4, 4 ] : MiR12-1-164-15.7-ss
2011-03-23    construct: MiR12-1-164-15.8
2011-04-26    expression [ 4, 4 ] : MiR12-1-164-15.8-ss
2011-03-23    construct: MiR12-6-164-15.9
2011-04-26    expression [ 5, 5 ] : MiR12-6-164-15.9-ss
2012-04-25    expression [ 4, 4 ] : MiR12-6-164-15.9-SeM-R1
2012-05-21    purification [44.0 mg]: MiR12-6-164-15.9-SeM-R1-Ni
2012-05-22    purification [9.6 mg]: MiR12-6-164-15.9-SeM-R1-GF
2012-05-30     aggregation screening [monomer,25.82 kD,37.3%|monomer,26.33 kD,55.1%]
2012-05-30     MiR12.021 @ HWI; 0.45 ml at 7.57 mg/mL
2012-05-30     MiR12.022 @ Rutgers; 0.4 ml at 7.57 mg/mL
2011-03-23    construct: MiR12-6-164-15.10
2011-04-26    expression [ 5, 5 ] : MiR12-6-164-15.10-ss
2011-03-23    construct: MiR12-1-158-15.11
2011-04-26    expression [ 5, 5 ] : MiR12-1-158-15.11-ss
2012-04-25    expression [ 4, 5 ] : MiR12-1-158-15.11-SeM-R1
2012-05-21    purification [33.6 mg]: MiR12-1-158-15.11-SeM-R1-Ni
2012-05-22    purification [10.2 mg]: MiR12-1-158-15.11-SeM-R1-GF
2012-05-30     aggregation screening [monomer,19.83 kD,97.0%]
2012-05-30     MiR12.023 @ HWI; 0.45 ml at 7.84 mg/mL
2012-05-30     MiR12.024 @ Columbia; 0.35 ml at 7.84 mg/mL
2011-03-23    construct: MiR12-1-158-15.12
2011-04-26    expression [ 5, 5 ] : MiR12-1-158-15.12-ss
2011-04-13    construct: MiR12-6-181-15.15
2011-04-26    expression [ 5, 5 ] : MiR12-6-181-15.15-ss
2011-04-13    construct: MiR12-6-181-15.16
2011-04-26    expression [ 5, 5 ] : MiR12-6-181-15.16-ss
2012-04-03    expression [ 4, 5 ] : MiR12-6-181-15.16-NC5a
2012-04-24    purification [16.1 mg]: MiR12-6-181-15.16-NC5a-GF
2012-05-04     MiR12.014 @ Rutgers; 0.04 ml at 1.141 mM
2012-06-06    HSQC [good]: MiR12.014.600MHz.20C
2012-05-04     MiR12.015 @ Rutgers; 0.7 ml at 1.141 mM
2012-04-10    expression [ 5, 4 ] : MiR12-6-181-15.16-SeMa
2012-05-21    purification [26.4 mg]: MiR12-6-181-15.16-SeMa-Ni
2012-05-22    purification [22.4 mg]: MiR12-6-181-15.16-SeMa-GF
2012-05-30     aggregation screening [monomer,19.6 kD,96.1%]
2012-05-30     MiR12.026 @ HWI; 0.45 ml at 8.02 mg/mL
2012-05-30     MiR12.027 @ Columbia; 1.85 ml at 8.02 mg/mL
2011-04-13    construct: MiR12-11-158-15.19
2011-04-26    expression [ 4, 4 ] : MiR12-11-158-15.19-ss
2012-04-25    expression [ 4, 4 ] : MiR12-11-158-15.19-SeM-R1
2012-05-21    purification [19.8 mg]: MiR12-11-158-15.19-SeM-R1-Ni
2012-05-22    purification [2.0 mg]: MiR12-11-158-15.19-SeM-R1-GF
2012-05-30     aggregation screening [dimer,34.57 kD,94.4%]
2012-05-30     MiR12.025 @ HWI; 0.45 ml at 3.7 mg/mL
2011-04-13    construct: MiR12-11-158-15.20
2011-04-26    expression [ 5, 2 ] : MiR12-11-158-15.20-ss


Available samples:

LB samples
mir12.017 @ Miami MiR12.016 @ Rutgers MiR12.018 @ Rutgers


SeMet samples
MiR12.022 @ Rutgers MiR12.021 @ HWI
MiR12.019 @ HWI MiR12.025 @ HWI MiR12.023 @ HWI MiR12.020 @ Rutgers MiR12.001 @ Columbia
MiR12.013 @ Phillips MiR12.003 @ Rutgers MiR12.024 @ Columbia MiR12.026 @ HWI MiR12.027 @ Columbia
MiR12.002 @ HWI


NC samples
MiR12.011 @ Miami MiR12.012 @ UGA MiR12.007 @ Rutgers mir12.006 @ Miami


NC5 samples
MiR12.005 @ Rutgers mir12.009 @ Rutgers MiR12.004 @ Rutgers MiR12.015 @ Rutgers MiR12.010 @ UGA
MiR12.014 @ Rutgers mir12.008 @ Miami


E-mail communications associated with this target:

  2012-05-09
  2012-05-30
  2012-05-31
  2012-06-01
  2012-06-04
  2012-06-06
  2012-06-06