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OR15  

database refs UniProt: Unknown_4108 (Unknown_4108)
status NMR structure and crystal hits
structures 2KL8 (NMR)  
properties length: 76 aa   |   mass: 8.85 kD   |   pI: 5.50   |   ext: 2980   |   organism: Other
classes PSI-2, Community Outreach, De Novo Design, D. Baker, PSI-Biology
projects ferredoxin fold     Community Outreach     D Baker projects     Design projects    
best PDB match calculating ...
informatics DISOPRED2 data   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic


OR15

       PDB seq homology



Constructs and Most Advanced Status
   
OR15-29.*



most recent record
7 years, 3 days ago --     aggregation screening [aggregated,307.3 kD,85.92%]

2009-02-20    target: OR15
2009-02-20    construct: OR15-29.1
2009-02-26    expression [ 2, 5 ] : OR15-29.1-NC5a
2009-03-10    purification [19.9 mg]: OR15-29.1-NC5a-Ni
2009-03-12    purification [4.7 mg]: OR15-29.1-NC5a-GF
2009-03-17     OR15.001 @ Rutgers; 0.035 ml at 0.732 mM
2009-09-07    HSQC [promising]: OR15.001.600MHz.20C
2009-03-18     or15.002 @ UGA; 0.45 ml at 0.732 mM
2009-08-19    HSQC [good]: OR15.002.500MHz.20C
2009-05-19    expression [ 3, 5 ] : OR15-29.1-NCa
2009-05-28    purification [30.2 mg]: OR15-29.1-NCa-GF
2009-06-23     aggregation screening [monomer,11.7 kD,94.8%]
2009-06-03     or15.003 @ UGA; 0.3 ml at 0.57 mM
2009-06-30     NMR structure: 2kl8 (PDB)
2009-06-03     or15.004 @ Rutgers; 0.35 ml at 0.98 mM
2009-06-22     OR15.005 @ Rutgers; 0.3 ml at 0.98 mM
2009-05-19    expression [ 3, 5 ] : OR15-29.1-NC5b
2009-06-26    purification [16.7 mg]: OR15-29.1-NC5b-GF
2009-06-30     OR15.006 @ Rutgers; 0.5 ml at 0.87 mM
2009-06-30     or15.007 @ Rutgers; 0.1 ml at 0.87 mM
2009-07-06     OR15.008 @ UGA; 0.5 ml at 0.87 mM
2009-05-19    expression [ 3, 5 ] : OR15-29.1-NCb
2009-05-28    purification [30.2 mg]: OR15-29.1-NCb-GF
2009-05-19    expression [ 3, 5 ] : OR15-29.1-NC5c
2009-06-26    purification [16.7 mg]: OR15-29.1-NC5c-GF
2010-11-03    expression [ 3, 5 ] : OR15-29.1-SeM-R1
2010-11-08    purification [13.2 mg]: OR15-29.1-SeM-R1-GF
2010-11-16     aggregation screening [aggregated,307.3 kD,85.92%]
2010-11-16     or15.009 @ Rutgers; 0.6 ml at 9.4 mg/mL
2010-11-16     OR15.010 @ HWI; 0.45 ml at 9.4 mg/mL


Available samples:

SeMet samples
OR15.010 @ HWI or15.009 @ Rutgers


NC samples
or15.004 @ Rutgers OR15.005 @ Rutgers or15.003 @ UGA


NC5 samples
or15.002 @ UGA OR15.001 @ Rutgers or15.007 @ Rutgers OR15.006 @ Rutgers OR15.008 @ UGA


E-mail communications associated with this target:

  2012-04-25