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OR150   (HaloTag2)

database refs UniProt: Unknown_5202 (Unknown_5202)
status X-Ray structure
structures 4KAA (Xray)   4KAC (Xray)   4KYV (Xray)  
properties length: 296 aa   |   mass: 33.33 kD   |   pI: 4.84   |   ext: 59930   |   organism: Other
classes Community Outreach, PSI-Biology
projects HaloTag     Community Outreach    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures53474 details   PF12697 details   3.40.50.1820 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    CATH 3.40.50.1820
    SuperFamily 53474
    PFAM PF12697

OR150

       PDB seq homology



Constructs and Most Advanced Status
   
OR150-21.*

   
OR150-15.*



most recent record
3 years, 28 days ago --     HSQC [not scored]

2011-11-11    target: OR150
2012-02-09    construct: OR150-15.4
2012-03-02    expression [ 5, 5 ] : OR150-15.4-ss
2012-04-11    expression [ 5, 4 ] : OR150-15.4-SeM-R1
2012-05-01    purification [54.0 mg]: OR150-15.4-SeM-R1-Ni
2012-05-02    purification [72.6 mg]: OR150-15.4-SeM-R1-GF
    OR150.001 @ HWI; 0.45 ml at 11.25 mg/mL
    OR150.002 @ Columbia; 2 ml at 11.25 mg/mL
2013-04-22     X-Ray structure: 4kaa (PDB)
    OR150.003 @ Rutgers; 1.75 ml at 11.25 mg/mL
    OR150.004 @ HWI; 0.45 ml at 10.9 mg/mL
    OR150.005 @ Columbia; 2 ml at 10.9 mg/mL
2013-04-22     X-Ray structure: 4kac (PDB)
2013-05-29     X-Ray structure: 4kyv (PDB)
    OR150.006 @ Rutgers; Unknown volume and concentration
2012-04-11    expression [ 5, 3 ] : OR150-15.4-NC5a
2012-05-10    purification [63.1 mg]: OR150-15.4-NC5a-GF
2012-05-25     OR150.007 @ Rutgers; 0.04 ml at 1.3 mM
2012-06-06    HSQC [good]: OR150.007.600MHz.20C
2012-05-31     OR150.008 @ Rutgers; 0.04 ml at 1.3 mM
2012-06-25    HSQC [good]: OR150.008.600MHz.20C
2012-07-10     OR150.013 @ Rutgers; 0.04 ml at 0.814 mM
2012-08-23    HSQC [good]: OR150.013.600MHz.20C
2012-07-10     OR150.012 @ Rutgers; 0.04 ml at 0.876 mM
2012-08-23    HSQC [good]: OR150.012.600MHz.20C
2012-07-10     OR150.010 @ Rutgers; 0.04 ml at 0.921 mM
2012-08-23    HSQC [good]: OR150.010.600MHz.20C
2012-07-10     OR150.011 @ Rutgers; 0.04 ml at 0.321 mM
2012-08-23    HSQC [poor]: OR150.011.600MHz.20C
2012-11-27    expression [ 5, 4 ] : OR150-15.4-NC-R1
2012-12-05    purification [43.6 mg]: OR150-15.4-NC-R1-GF
2012-12-20     OR150.014 @ Rutgers; 0.7 ml at 1.098 mM
2012-08-23    HSQC [good]: OR150.014.600MHz.20C
2014-04-29    HSQC [not scored]: OR150.014.600MHz.25C
2012-12-20     OR150.009 @ Rutgers; 0.3 ml at 1.1 mM
2012-12-05    purification [18.3 mg]: OR150-15.4-NC-R1-Ni
2013-04-05    expression [ 5, 4 ] : OR150-15.4-DNC-ILVa
2012-02-09    construct: OR150-21.2
2012-03-02    expression [ 5, 5 ] : OR150-21.2-ss
2012-02-09    construct: OR150-15.3
2012-03-02    expression [ 5, 5 ] : OR150-15.3-ss
2012-02-09    construct: OR150-21.1
2012-03-02    expression [ 4, 5 ] : OR150-21.1-ss


Available samples:

SeMet samples
OR150.005 @ Columbia OR150.003 @ Rutgers OR150.004 @ HWI OR150.006 @ Rutgers OR150.002 @ Columbia
OR150.001 @ HWI


NC samples
OR150.014 @ Rutgers OR150.009 @ Rutgers


NC5 samples
OR150.008 @ Rutgers OR150.007 @ Rutgers
OR150.011 @ Rutgers OR150.012 @ Rutgers OR150.010 @ Rutgers OR150.013 @ Rutgers


E-mail communications associated with this target:

  2012-05-09
  2012-05-10
  2012-05-10
  2012-05-16