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OR165   (1trg_4)

database refs UniProt: Unknown_5233 (Unknown_5233)
status X-Ray structure
structures 4F2V (Xray)  
properties length: 264 aa   |   mass: 30.14 kD   |   pI: 5.70   |   ext: 37930   |   organism: Other
classes Community Outreach, PSI-Biology
projects serine hydrolase design     Community Outreach     D Baker projects     Design projects    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF00303 details   3.30.572.10 details   55831 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    SuperFamily 55831
    CATH 3.30.572.10
    PFAM PF00303

OR165

       PDB seq homology



Constructs and Most Advanced Status
   
OR165-29.*



most recent record
5 years, 2 days ago --     HSQC [promising]; HSQC [promising]; HSQC [promising]; HSQC [poor]

2012-01-30    target: OR165
2012-02-06    construct: OR165-29.1
2012-02-29    expression [ 5, 4 ] : OR165-29.1-NC5a
2012-04-09    purification [53.2 mg]: OR165-29.1-NC5a-GF
2012-04-17     OR165.004 @ Rutgers; 0.04 ml at 1.51 mM
2012-06-06    HSQC [promising]: OR165.004.600MHz.20C
2012-04-17     OR165.005 @ Rutgers; 0.01 ml at 1.51 mM
2012-07-10     OR165.007 @ Rutgers; 0.04 ml at 0.742 mM
2012-08-23    HSQC [promising]: OR165.007.600MHz.20C
2012-07-10     OR165.008 @ Rutgers; 0.04 ml at 0.628 mM
2012-08-23    HSQC [promising]: OR165.008.600MHz.20C
2012-07-10     OR165.009 @ Rutgers; 0.04 ml at 0.677 mM
2012-08-23    HSQC [promising]: OR165.009.600MHz.20C
2012-07-10     OR165.006 @ Rutgers; 0.04 ml at 0.805 mM
2012-08-23    HSQC [poor]: OR165.006.600MHz.20C
2012-02-29    expression [ 5, 3 ] : OR165-29.1-SeM-R1
2012-03-05    purification [35.4 mg]: OR165-29.1-SeM-R1-Ni
2012-03-06    purification [46.4 mg]: OR165-29.1-SeM-R1-GF
2012-03-13     aggregation screening [dimer,62.32 kD,98.1%]
2012-04-05     OR165.001 @ HWI; 0.45 ml at 8.55 mg/mL
2012-05-07     OR165.003 @ Rutgers; 1.5 ml at 8.55 mg/mL
2012-04-05     OR165.002 @ Columbia; 2 ml at 8.55 mg/mL
2012-05-08     X-Ray structure: 4f2v (PDB)


Available samples:

SeMet samples
OR165.002 @ Columbia OR165.001 @ HWI OR165.003 @ Rutgers


NC5 samples
OR165.007 @ Rutgers OR165.009 @ Rutgers
OR165.005 @ Rutgers OR165.006 @ Rutgers OR165.008 @ Rutgers OR165.004 @ Rutgers