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OR258   (ploop2x3_50)

database refs UniProt: Unknown_5338 (Unknown_5338)
status X-Ray structure    work stopped (2012-11-30; Requested by Nominator)
structures 4J29 (Xray)  
properties length: 126 aa   |   mass: 14.67 kD   |   pI: 4.96   |   ext: 2980   |   organism: Other
classes Community Outreach, D. Baker, PSI-Biology
projects P-loop NTPase fold     D Baker projects     Design projects     Community Outreach    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures3.40.50.720 details   3.20.20.190 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    CATH 3.20.20.190
    CATH 3.40.50.720

OR258

       PDB seq homology



Constructs and Most Advanced Status
   
OR258-29.*



most recent record
4 years, 23 days ago --     X-Ray structure: 4j29

2012-05-04    target: OR258     work stopped (2012-11-30; Requested by Nominator)
2012-05-04    construct: OR258-29.1     work stopped (2012-11-30; Requested by Nominator)
2012-05-15    expression [ 5, 5 ] : OR258-29.1-NCa
2012-06-26    purification [25.6 mg]: OR258-29.1-NCa-Ni
2012-06-26    purification [12.5 mg]: OR258-29.1-NCa-GF
2012-07-06     OR258.003 @ Rutgers; 0.3 ml at 0.89 mM
2012-07-06     OR258.004 @ Rutgers; 0.7 ml at 0.89 mM
2012-05-15    expression [ 5, 5 ] : OR258-29.1-NC5a
2012-06-26    purification [22.4 mg]: OR258-29.1-NC5a-Ni
2012-06-26    purification [16.0 mg]: OR258-29.1-NC5a-GF
2012-07-06     OR258.005 @ UGA; 0.5 ml at 0.89 mM
2012-07-06     OR258.006 @ Rutgers; 0.5 ml at 0.89 mM
2012-05-15    expression [ 5, 5 ] : OR258-29.1-SeM-R1
2012-05-23    purification [4.4 mg]: OR258-29.1-SeM-R1-GF
2012-05-30     aggregation screening [monomer,14.1 kD,94.2%]
2012-05-29     OR258.001 @ Columbia; 2 ml at 6.3 mg/mL
2013-02-04     X-Ray structure: 4j29 (PDB)
2012-05-29     OR258.002 @ HWI; 0.45 ml at 6.3 mg/mL
2012-10-18    expression [ 4, 4 ] : OR258-29.1-SeM-R2
2012-11-27    purification [26.0 mg]: OR258-29.1-SeM-R2-GF
2012-12-04     aggregation screening [monomer,14.04 kD,94.1%]
2012-11-30     OR258.007 @ Rutgers; 2 ml at 6.2 mg/mL
2012-11-30     OR258.008 @ Rutgers; 0.7 ml at 6.2 mg/mL


Available samples:

SeMet samples
OR258.001 @ Columbia OR258.007 @ Rutgers OR258.008 @ Rutgers OR258.002 @ HWI


NC samples
OR258.003 @ Rutgers
OR258.004 @ Rutgers


NC5 samples
OR258.005 @ UGA OR258.006 @ Rutgers


E-mail communications associated with this target:

  2012-05-30
  2012-05-31
  2012-06-01
  2012-06-04
  2012-12-20
  2012-12-21