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OR277   (design_13_8)

database refs UniProt: Unknown_5360 (Unknown_5360)
status X-Ray structure
structures 4NEY (Xray)  
properties length: 165 aa   |   mass: 18.94 kD   |   pI: 5.52   |   ext: 16960   |   organism: Other
classes Community Outreach, D. Baker, PSI-Biology
projects ferredoxin fold     Community Outreach     D Baker projects     Design projects    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic


OR277

       PDB seq homology



Constructs and Most Advanced Status
   
OR277-21.*



most recent record
4 years, 9 days ago --     aggregation screening [dimer,39.48 kD,98.5%]

2012-05-31    target: OR277
2012-05-31    construct: OR277-21.1
2012-06-13    expression [ 5, 3 ] : OR277-21.1-NC5a
2012-09-05    purification [81.2 mg]: OR277-21.1-NC5a-Ni
2012-09-05    purification [92.6 mg]: OR277-21.1-NC5a-GF
2012-11-12     OR277.001 @ Rutgers; 0.04 ml at 0.527 mM
2012-12-10    HSQC [promising]: OR277.001.600MHz.20C
2012-11-12     OR277.002 @ Rutgers; 4.46 ml at 0.527 mM
2012-06-13    expression [ 5, 0 ] : OR277-21.1-SeM-R1
2013-02-19    expression [ 5, 5 ] : OR277-21.1-SeM-R2
2013-03-11    purification [74.8 mg]: OR277-21.1-SeM-R2-GF
2013-03-19     aggregation screening [dimer,39.48 kD,98.5%]
2013-03-15     OR277.003 @ HWI; 0.45 ml at 9.12 mg/mL
2013-03-15     OR277.004 @ Rutgers; 2 ml at 9.12 mg/mL
    X-Ray structure: 4ney (PDB)
2013-03-15     OR277.005 @ Rutgers; 4.5 ml at 9.12 mg/mL


Available samples:

SeMet samples
OR277.005 @ Rutgers OR277.003 @ HWI OR277.004 @ Rutgers


NC5 samples
OR277.001 @ Rutgers OR277.002 @ Rutgers


E-mail communications associated with this target:

  2013-03-20
  2013-03-20
  2013-03-26