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OR301   (OSH55.4_H1.2)

database refs UniProt: Unknown_5369 (Unknown_5369)
status X-Ray structure
structures 4J4Z (Xray)  
properties length: 159 aa   |   mass: 16.83 kD   |   pI: 7.88   |   ext: 15470   |   organism: Other
classes Community Outreach, D. Baker, PSI-Biology
projects serine hydrolase design     Community Outreach     D Baker projects     Design projects    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures52949 details   PF01661 details   3.40.220.10 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    CATH 3.40.220.10
    SuperFamily 52949
    PFAM PF01661

OR301

       PDB seq homology



Constructs and Most Advanced Status
   
OR301-29.*



most recent record
4 years, 20 days ago --     X-Ray structure: 4j4z

2012-07-06    target: OR301
2012-07-09    construct: OR301-29.1
2012-07-19    expression [ 0, 0 ] : OR301-29.1-SeM-R1
2012-07-19    expression [ 0, 0 ] : OR301-29.1-NC5a
2012-08-01    expression [ 0, 0 ] : OR301-29.1-SeM-R2
2012-08-01    expression [ 3, 5 ] : OR301-29.1-NC5b
2012-09-05    purification [33.8 mg]: OR301-29.1-NC5b-Ni
2012-09-05    purification [26.9 mg]: OR301-29.1-NC5b-GF
2012-11-12     OR301.001 @ Rutgers; 0.04 ml at 0.224 mM
2012-12-10    HSQC [good]: OR301.001.600MHz.20C
2012-11-12     OR301.002 @ Rutgers; 3.96 ml at 0.224 mM
2012-08-30    expression [ 5, 5 ] : OR301-29.1-SeM-R3
2012-09-25    purification [29.2 mg]: OR301-29.1-SeM-R3-Ni
2012-09-26    purification [19.6 mg]: OR301-29.1-SeM-R3-GF
2012-10-03     aggregation screening [monomer,19.6 kD,80.6%]
2012-10-02     OR301.003 @ HWI; 0.45 ml at 8.79 mg/mL
2012-10-02     OR301.004 @ Columbia; 1.15 ml at 8.79 mg/mL
2013-02-07     X-Ray structure: 4j4z (PDB)
2012-10-02    expression [ -1, -1 ] : OR301-29.1-LBa
2012-10-02    purification [10.8 mg]: OR301-29.1-LBa-NI
2012-10-25     aggregation screening [#VALUE!,. kD,0%]
2012-10-08     OR301.005 @ Rutgers; 0.05 ml at 12 mg/mL
2012-10-08     OR301.006 @ HWI; 0.45 ml at 12 mg/mL
2012-10-08     OR301.007 @ Columbia; 0.4 ml at 12 mg/mL


Available samples:

LB samples
OR301.005 @ Rutgers OR301.007 @ Columbia OR301.006 @ HWI


SeMet samples
OR301.003 @ HWI OR301.004 @ Columbia


NC5 samples
OR301.002 @ Rutgers OR301.001 @ Rutgers


E-mail communications associated with this target:

  2012-10-04
  2012-10-10
  2012-11-07
  2012-11-08
  2012-11-08
  2012-11-13