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OR414   (LFR11)

database refs UniProt: Unknown_5499 (Unknown_5499)
status NMR structure
structures 2MQ8 (NMR)  
properties length: 104 aa   |   mass: 12.03 kD   |   pI: 5.06   |   ext: 6990   |   organism: Other
classes Community Outreach, PSI-Biology
projects ferredoxin fold     D Baker projects     Design projects     Community Outreach    
best PDB match calculating ...
informatics Zn binding sites: Analysis not performed.
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic


OR414

       PDB seq homology



Constructs and Most Advanced Status
   
OR414-21.*



most recent record
3 years, 15 days ago --     NMR structure: 2mq8

2013-02-19    target: OR414
2013-02-20    construct: OR414-21.1
2013-03-06    expression [ 5, 4 ] : OR414-21.1-ss
2013-03-06    expression [ 5, 3 ] : OR414-21.1-ss2
2013-03-11    expression [ -1, -1 ] : OR414-21.1-NC5a
2013-03-20    purification [1.0 mg]: OR414-21.1-NC5a-GF
2013-03-20     OR414.001 @ Rutgers; 0.1 ml at 10 mg/mL
2013-04-12    HSQC [good]: OR414.001.600MHz.20C
2013-03-27    expression [ 5, 5 ] : OR414-21.1-SeMa
2013-04-22    purification [51.8 mg]: OR414-21.1-SeMa-GF
2013-05-07     aggregation screening [monomer,13.92 kD,97.2%]
2013-04-25     OR414.002 @ HWI; 0.45 ml at 8.9 mg/mL
2013-04-25     OR414.003 @ Rutgers; 2 ml at 8.9 mg/mL
2013-04-25     OR414.004 @ Rutgers; 3 ml at 8.9 mg/mL
2013-05-08    expression [ 5, 5 ] : OR414-21.1-NC-R1
2013-05-13    purification [84.8 mg]: OR414-21.1-NC-R1-Ni
2013-05-13    purification [67.1 mg]: OR414-21.1-NC-R1-GF
2013-05-15     OR414.005 @ Rutgers; 0.3 ml at 0.885 mM
2013-05-15     OR414.006 @ Rutgers; 4.7 ml at 0.885 mM
2014-06-12     NMR structure: 2mq8 (PDB)
2013-10-16    expression [ 3, 5 ] : OR414-21.1-NC5b
2013-10-31    purification [70.8 mg]: OR414-21.1-NC5b-Ni
2013-11-05    purification [70.0 mg]: OR414-21.1-NC5b-GF
2013-11-08     OR414.007 @ Rutgers; 0.05 ml at 1 mM
2014-04-28    HSQC [not scored]: OR414.007.600MHz.27C
2014-03-11    HSQC [not scored]: OR414.007.600MHz.20C
2013-11-08     OR414.008 @ Rutgers; 2.5 ml at 1 mM
2013-12-18     OR414.009 @ UGA; 1 ml at 1 mM
2014-01-08     OR414.010 @ Rutgers; 0.5 ml at 1 mM


Available samples:

SeMet samples
OR414.003 @ Rutgers OR414.002 @ HWI OR414.004 @ Rutgers


NC samples
OR414.006 @ Rutgers OR414.005 @ Rutgers


NC5 samples
OR414.010 @ Rutgers OR414.001 @ Rutgers OR414.009 @ UGA OR414.008 @ Rutgers OR414.007 @ Rutgers


E-mail communications associated with this target:

  2013-05-08
  2013-05-08
  2013-05-09
  2013-05-09
  2013-12-19
  2013-12-19