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OR43   (RH13-SA-KM)

database refs UniProt: Unknown_5049 (Unknown_5049)
status X-Ray structure    work stopped (2013-07-25; target work stopped by D. Baker)
structures 3VB8 (Xray)  
properties length: 161 aa   |   mass: 19.20 kD   |   pI: 5.17   |   ext: 38390   |   organism: Other
classes Community Outreach, D. Baker, PSI-Biology
projects HEWL binding design     Community Outreach     D Baker projects     Design projects    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF11646 details  
commentsdetermine structure of HEWL-bound complex
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    PFAM PF11646

OR43

       PDB seq homology



Constructs and Most Advanced Status
   
OR43-29.*



most recent record
5 years, 26 days ago --     X-Ray structure: 3VB8

2011-01-07    target: OR43     work stopped (2013-07-25; target work stopped by D. Baker)
2011-01-10    construct: OR43-29.1
2011-01-25    expression [ 2, 5 ] : OR43-29.1-SeMa
2011-02-03    purification [0.0 mg]: OR43-29.1-SeMa-GF
2011-01-25    expression [ 2, 5 ] : OR43-29.1-NC5a
2011-02-02    purification [22.9 mg]: OR43-29.1-NC5a-Ni
2011-02-04    purification [5.1 mg]: OR43-29.1-NC5a-GF
2011-02-22     OR43.002 @ Rutgers; 0.04 ml at 0.094 mM
2011-02-11     OR43.001 @ Rutgers; 0.04 ml at 0.094 mM
2011-02-16    HSQC [poor]: OR43.001.600MHz.20C
2011-02-11    expression [ 2, 5 ] : OR43-29.1-SeM-R1
2011-02-21    purification [8.0 mg]: OR43-29.1-SeM-R1-GF
2011-03-03     aggregation screening [monomer,25.03 kD,99.2%]
2011-03-02     OR43.003 @ HWI; 0.45 ml at 7.82 mg/mL
2011-12-31     X-Ray structure: 3VB8 (PDB)
2011-03-15    expression [ 2, 5 ] : OR43-29.1-SeMc
2011-03-22    purification [0.0 mg]: OR43-29.1-SeMc-GF
2011-03-15    expression [ 2, 5 ] : OR43-29.1-SeMb
2011-03-22    purification [0.0 mg]: OR43-29.1-SeMb-GF
2011-03-23    expression [ 2, 5 ] : OR43-29.1-NC5b
2011-04-04    purification [21.0 mg]: OR43-29.1-NC5b-Ni
2011-04-04    purification [14.1 mg]: OR43-29.1-NC5b-GF
2011-03-23    expression [ 2, 5 ] : OR43-29.1-NC5c
2011-04-04    purification [21.0 mg]: OR43-29.1-NC5c-Ni
2011-04-04    purification [14.1 mg]: OR43-29.1-NC5c-GF
2011-04-08     OR43.007 @ Rutgers; 0.2 ml at 0.56 mM
2011-10-05     OR43.006 @ Rutgers; 0.5 ml at 0.56 mM
2011-10-05     OR43.004 @ Rutgers; Unknown volume and concentration
2011-10-05     OR43.005 @ Rutgers; Unknown volume and concentration
2011-04-01    expression [ -1, -1 ] : OR43-29.1-LB
2011-04-01    purification [0.0 mg]: OR43-29.1-LB-GF
2011-04-06     OR43.008 @ HWI; 0.45 ml at 5 mg/mL
2011-04-06     OR43.009 @ Columbia; 0.55 ml at 5 mg/mL
2011-10-04    expression [ 1, 4 ] : OR43-29.1-SeM-R3
2011-10-10    purification [8.4 mg]: OR43-29.1-SeM-R3-GF
2011-10-25     aggregation screening [monomer,23.18 kD,93.1%]
2011-10-20     OR43.010 @ Rutgers; 0.85 ml at 7.58 mg/mL
2011-10-04    expression [ 1, 4 ] : OR43-29.1-SeM-R2
2011-10-10    purification [8.4 mg]: OR43-29.1-SeM-R2-GF


Available samples:

LB samples
OR43.008 @ HWI OR43.009 @ Columbia


SeMet samples
OR43.010 @ Rutgers OR43.003 @ HWI


NC5 samples
OR43.004 @ Rutgers
OR43.006 @ Rutgers OR43.007 @ Rutgers OR43.002 @ Rutgers OR43.005 @ Rutgers OR43.001 @ Rutgers