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OR54   (ECH14)

database refs UniProt: Unknown_5060 (Unknown_5060)
status X-Ray structure
structures 3UAK (Xray)  
properties length: 398 aa   |   mass: 43.78 kD   |   pI: 5.26   |   ext: 42860   |   organism: Other
classes Community Outreach, D. Baker, PSI-Biology
projects cysteine esterases     Community Outreach     D Baker projects     Design projects    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures3.90.1150.10 details   PF00155 details   3.40.640.10 details   53383 details  
commentsapo, + tyrosine substrate, + coumarin ester structures requested
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    SuperFamily 53383
    PFAM PF00155
    CATH 3.40.640.10
    CATH 3.90.1150.10

OR54

       PDB seq homology



Constructs and Most Advanced Status
   
OR54-29.*



most recent record
5 years, 7 days ago --     X-Ray structure: 3uak

2011-01-07    target: OR54
2011-01-10    construct: OR54-29.1
2011-02-09    expression [ 5, 5 ] : OR54-29.1-SeM-R1
2011-02-14    purification [118.8 mg]: OR54-29.1-SeM-R1-GF
2011-02-23     aggregation screening [trimer,152.2 kD,53.4%|aggregated,1094 kD,45.07%]
2011-09-20     or54.006 @ Columbia; 2 ml at 9.9 mg/mL
2011-10-21     X-Ray structure: 3uak (PDB)
2011-02-22     OR54.001 @ Columbia; 2 ml at 9.9 mg/mL
2011-02-22     OR54.002 @ HWI; 0.45 ml at 9.9 mg/mL
2011-02-22     OR54.003 @ Rutgers; 8 ml at 9.9 mg/mL
2011-02-09    expression [ 5, 5 ] : OR54-29.1-NC5a
2011-02-14    purification [191.5 mg]: OR54-29.1-NC5a-Ni
2011-02-15    purification [130.6 mg]: OR54-29.1-NC5a-GF
2011-02-22     OR54.005 @ Rutgers; 1.5 ml at 1.267 mM
2011-02-22     OR54.004 @ Rutgers; 0.04 ml at 1.267 mM
2011-03-03    HSQC [poor]: OR54.004.600MHz.20C


Available samples:

SeMet samples
OR54.003 @ Rutgers or54.006 @ Columbia OR54.002 @ HWI OR54.001 @ Columbia


NC5 samples
OR54.005 @ Rutgers
OR54.004 @ Rutgers