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SiR5  

database refs UniProt: Q5LST8 (Q5LST8_SILPO)   |   NCBI gene: 3192768   |   refSeq: YP_166917.1
status NMR structure
structures 2OA4 (NMR)  
properties length: 93 aa   |   mass: 10.40 kD   |   pI: 9.99   |   ext: 15470   |   organism: Silicibacter pomeroyi
classes PSI-2, BIG: Rutgers, NESG-BIG-STRUCTURE, PSI-Biology
best PDB match calculating ...
informatics Prof PHD data   |   DISOPRED2 data   |   Pxs: 0.274   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures1.10.10.10 details   PF06627 details   48295 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    SuperFamily 48295
    PFAM PF06627
    CATH 1.10.10.10

SiR5

       PDB seq homology



Constructs and Most Advanced Status
   
SiR5-21.*



most recent record
6 years, 11 days ago --     purification [24.2 mg]: SiR5-21.2-Na-GF; purification [6.0 mg]: SiR5-21.2-LBa-GF

2006-03-16    target: SiR5
2006-06-01    construct: SiR5-21.1
2006-06-09    expression [ 2, 2 ] : SiR5-21.1-ss
2006-06-01    construct: SiR5-21.2
2006-06-09    expression [ 3, 4 ] : SiR5-21.2-ss
2006-06-22    expression [ 5, 5 ] : SiR5-21.2-SeMa
2006-06-27    purification [0.0 mg]: SiR5-21.2-SeMa-GF
2006-06-22    expression [ 4, 5 ] : SiR5-21.2-NC5a
2006-06-27    purification [12.5 mg]: SiR5-21.2-NC5a-GF
2006-07-05     SiR5.001 @ UGA; 0.5 ml at 1.151 mM
2006-07-13    HSQC [good]: SiR5-21.2-NC5a-GF_SiR5.001_20
2006-07-19    expression [ 3, 5 ] : SiR5-21.2-NCa
2006-07-24    purification [9.1 mg]: SiR5-21.2-NCa-GF
2006-07-27     sir5.002 @ Individual; 0.7 ml at 1.04 mM
2006-12-14     NMR structure: 2OA4 (PDB)
2006-09-20    expression [ 3, 5 ] : SiR5-21.2-SeMb
2006-10-16    purification [19.6 mg]: SiR5-21.2-SeMb-Ni
2006-10-18    purification [16.0 mg]: SiR5-21.2-SeMb-GF
2006-11-16     aggregation screening [dimer,23.94 kD,96%]
2006-10-25     SiR5.003 @ Columbia; 1.2 ml at 6.72 mg/mL
2006-10-25     SiR5.004 @ HWI; 0.45 ml at 6.72 mg/mL
2006-09-20    expression [ 3, 5 ] : SiR5-21.2-SeMc
2006-10-16    purification [19.6 mg]: SiR5-21.2-SeMc-Ni
2006-10-18    purification [16.0 mg]: SiR5-21.2-SeMc-GF
2010-12-08    expression [ 3, 5 ] : SiR5-21.2-Na
2011-02-16    purification [24.2 mg]: SiR5-21.2-Na-GF
    SiR5.005 @ Powers; Unknown volume and concentration
2010-12-08    expression [ 3, 5 ] : SiR5-21.2-LBa
2011-02-16    purification [6.0 mg]: SiR5-21.2-LBa-GF
    SiR5.006 @ Powers; Unknown volume and concentration
2006-06-09     5-1-B-10-6.A3 @ Rutgers; Unknown volume and concentration
2006-06-09     5-1-B-10-6.B3 @ Rutgers; Unknown volume and concentration
2006-06-09     5-4-B-10-6.A3 @ Rutgers; Unknown volume and concentration
2006-06-09     5-4-B-10-6.B3 @ Rutgers; Unknown volume and concentration


Available samples:

LB samples
SiR5.006 @ Powers


SeMet samples
SiR5.004 @ HWI SiR5.003 @ Columbia


Labeling not defined samples
SiR5.005 @ Powers


NC samples
sir5.002 @ Individual


NC5 samples
SiR5.001 @ UGA