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SlR115   (yhhM)

database refs UniProt: Q5PJL7 (Q5PJL7_SALPA)   |   NCBI gene: 3175530   |   refSeq: YP_152548.1
status NMR structure and crystal hits
structures 2L0C (NMR)  
properties length: 123 aa   |   mass: 13.87 kD   |   pI: 10.38   |   ext: 4470   |   organism: Salmonella paratyphi
classes PSI-2, META Genomic, PSI-Biology, Hendrickson Multiple Crystal SAD
projects Hendrickson Multiple Crystal SAD    
best PDB match calculating ...
informatics Prof PHD data   |   DISOPRED2 data   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)   |   TM helices (details)
domain signaturesPF10694 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    PFAM PF10694

SlR115

       PDB seq homology



Constructs and Most Advanced Status
   
SlR115-36-117-21.*

   
SlR115-36-123-21.*



most recent record
4 years, 6 days ago --     aggregation screening [Aggregated,350.7 kD,50.0%]

2009-11-04    target: SlR115
2010-01-14    construct: SlR115-36-117-21.6
2010-01-28    expression [ 4, 5 ] : SlR115-36-117-21.6-ss
2010-01-14    construct: SlR115-36-123-21.9
2010-01-28    expression [ 4, 5 ] : SlR115-36-123-21.9-ss
2010-01-14    construct: SlR115-36-117-21.2
2010-01-28    expression [ 4, 5 ] : SlR115-36-117-21.2-ss
2010-02-02    expression [ 4, 5 ] : SlR115-36-117-21.2-NC5a
2010-02-02    expression [ 4, 5 ] : SlR115-36-117-21.2-SeMa
2010-02-18    purification [102.0 mg]: SlR115-36-117-21.2-SeMa-GF
2010-02-23     aggregation screening [Aggregated,0 kD,0%]
2010-02-22     slr115.001 @ Rutgers; 2 ml at 2.6 mg/mL
2010-02-22     SlR115.002 @ Rutgers; 0.45 ml at 2.6 mg/mL
2010-02-22     SlR115.003 @ Rutgers; 6.5 ml at 2.6 mg/mL
2010-01-14    construct: SlR115-36-123-21.3
2010-01-28    expression [ 4, 5 ] : SlR115-36-123-21.3-ss
2010-02-03    expression [ 4, 5 ] : SlR115-36-123-21.3-NC5a
2010-03-02    purification [47.4 mg]: SlR115-36-123-21.3-NC5a-GF
2010-03-23     aggregation screening [monomer,14.6 kD,97.4%]
2010-03-05     slr115.007 @ Rutgers; 2.95 ml at 0.711 mM
2010-03-05     SlR115.006 @ Rutgers; 0.04 ml at 0.711 mM
2010-06-07    HSQC [good]: SlR115.006.600MHz.20C
2010-02-09    expression [ 4, 5 ] : SlR115-36-123-21.3-SeMa
2010-03-01    purification [61.2 mg]: SlR115-36-123-21.3-SeMa-GF
2010-03-10     aggregation screening [monomer,16.3 kD,98.7%]
2010-03-05     SlR115.004 @ Columbia; 2 ml at 6.5 mg/mL
2010-03-05     SlR115.005 @ HWI; 0.45 ml at 6.5 mg/mL
2010-03-31    expression [ 5, 5 ] : SlR115-36-123-21.3-NCa
2010-04-05    purification [58.3 mg]: SlR115-36-123-21.3-NCa-GF
2010-04-07     SlR115.008 @ Rutgers; 0.3 ml at 0.49 mM
2010-06-07    HSQC [good]: SlR115.008.600MHz.20C
2010-04-07     slr115.009 @ Rutgers; 4.7 ml at 0.49 mM
2010-04-15     SlR115.012 @ PNNL; 0.3 ml at 0.49 mM
2010-06-30     NMR structure: 2l0c (PDB)
2010-04-06    expression [ 5, 5 ] : SlR115-36-123-21.3-NC5b
2010-04-12    purification [46.7 mg]: SlR115-36-123-21.3-NC5b-GF
2010-04-14     SlR115.010 @ PNNL; 0.5 ml at 0.5 mM
2010-06-07    HSQC [good]: SlR115.010.600MHz.25C
2010-04-14     slr115.011 @ Rutgers; 3.5 ml at 0.5 mM
2010-04-21     SlR115.013 @ UGA; 0.5 ml at 0.5 mM
2013-07-02    expression [ 4, 5 ] : SlR115-36-123-21.3-SeM-R1
2013-07-08    purification [22.3 mg]: SlR115-36-123-21.3-SeM-R1-GF
2013-07-16     aggregation screening [Aggregated,419.9 kD,29.4%]
2013-07-10     SlR115.014 @ Rutgers; 2 ml at 1.9 mg/mL
2013-07-10     SlR115.015 @ Rutgers; 0.5 ml at 1.9 mg/mL
2013-08-07    expression [ 5, 5 ] : SlR115-36-123-21.3-LBa
2013-08-12    purification [183.6 mg]: SlR115-36-123-21.3-LBa-GF
2013-08-20     aggregation screening [Aggregated,350.7 kD,50.0%]
2013-08-15     SlR115.016 @ HWI; 0.45 ml at 10.12 mg/mL
2013-08-15     SlR115.017 @ Columbia; 0.2 ml at 10.12 mg/mL


Available samples:

LB samples
SlR115.017 @ Columbia SlR115.016 @ HWI


SeMet samples
SlR115.002 @ Rutgers slr115.001 @ Rutgers SlR115.014 @ Rutgers
SlR115.005 @ HWI SlR115.015 @ Rutgers SlR115.004 @ Columbia SlR115.003 @ Rutgers


NC samples
slr115.009 @ Rutgers
SlR115.012 @ PNNL SlR115.008 @ Rutgers


NC5 samples
slr115.007 @ Rutgers slr115.011 @ Rutgers SlR115.010 @ PNNL
SlR115.013 @ UGA SlR115.006 @ Rutgers


E-mail communications associated with this target:

  2013-10-23
  2013-10-24
  2013-10-25